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The map label for this gene is nbaI

Identifier: 138896788

GI number: 138896788

Start: 3252850

End: 3253878

Strand: Reverse

Name: nbaI

Synonym: GTNG_3151

Alternate gene names: 138896788

Gene position: 3253878-3252850 (Counterclockwise)

Preceding gene: 162330293

Following gene: 138896787

Centisome position: 91.65

GC content: 58.41

Gene sequence:

>1029_bases
GTGAGCAGCGAACGCGATATATGGATTACGGAAGTGGCGCTTCGTGACGGGAGCCACGCGATCAACCACCAGTACACGGT
GGAGCAGGTGGTGAGCGTCGCACAAGCGCTGGACGAGGCCAATGTGCCGTACATTGAAGTTTCTCACGGGGATGGTCTAG
CCGGCTCTTCCCTGCAGTATGGACTGTCTCGGACGGACGAGATGGAGCTGATTGAGGCGGCTGTCTCTGTATGCAAAAAG
GCGAAGATCGCTGTCCTGCTACTGCCCGGCATTGGCACGATCCGAGAGTTGGACGCCGCGGCCAAACGGGGAGTCAAGAT
GGTGCGGATTGCTACCCATGTCACCGAAGCGGACATCTCCGCGCAGCATATCACCCGTGCCAAGGAGTTGGGGCTGGAGG
CTGTCGGTTTCTTGATGATGTCCCACATGGCGTCAACGGAGAAGCTGGTGGAGCAGGCTAAGCTAATGGAAAGCTATGGT
GCCGACGTGGTGTACGTGGTGGACTCTGCTGGTGCGCTTTTGCCGCACGAGGTGACGGAGCGCATCCGCGCTTTGAAACA
GAGCCTAGGCATCGAGATCGGATTCCACGGCCACAACAATCTCTCGTTGGCGATGGCCAATACGTTGGCTGCCATTCAGG
AGGGAGCGACACGCATCGATGGTAGCGTCCGTTGTCTGGGAGCCGGAGCGGGTAACACTCAGACGGAGGTGCTGGTGGCT
GTGCTGGACCGCATAGGGCTCAAAACTGGTGTCGATCTGTACAAGATGATGGACCTAGCAGAAGAGATCGTGGCGCCCAT
CCTCCAGGTTCCACAGGAGATCACCCGGGACAGCCTGATCCTCGGCTATGCAGGTGTTTACTCCAGTTTCCGCTTGCACG
CGGAGCGTGCAGCCAAACGGTTCGGTGTCGACTCTCGTGACATCCTTGTTGAGCTAGGCAAACGCAAGGTCGTTGGCGGC
CAGGAGGACATGATCATCGACATTGCGGCTGAAATCGCAAGCAAAAAGACGGCCACCGGGGCACGATGA

Upstream 100 bases:

>100_bases
CACTGCCATCAAGGTGGCGGAGGAATGGGTTCAACACCGTGTGAAAGCTGCTTCGGTTTGATGGCCGCTTCGGCACGACG
TGCGAGAAGGAGGAGAGAGA

Downstream 100 bases:

>100_bases
AGGGGGATTCACATGACCAATGAACTGTACCGACAACTTGCCGAGCGGCTTGCAGTGGCGGAGCGGGAGCATCGGACCGT
GACGAAGCTCACGGCGGAAT

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSSERDIWITEVALRDGSHAINHQYTVEQVVSVAQALDEANVPYIEVSHGDGLAGSSLQYGLSRTDEMELIEAAVSVCKK
AKIAVLLLPGIGTIRELDAAAKRGVKMVRIATHVTEADISAQHITRAKELGLEAVGFLMMSHMASTEKLVEQAKLMESYG
ADVVYVVDSAGALLPHEVTERIRALKQSLGIEIGFHGHNNLSLAMANTLAAIQEGATRIDGSVRCLGAGAGNTQTEVLVA
VLDRIGLKTGVDLYKMMDLAEEIVAPILQVPQEITRDSLILGYAGVYSSFRLHAERAAKRFGVDSRDILVELGKRKVVGG
QEDMIIDIAAEIASKKTATGAR

Sequences:

>Translated_342_residues
MSSERDIWITEVALRDGSHAINHQYTVEQVVSVAQALDEANVPYIEVSHGDGLAGSSLQYGLSRTDEMELIEAAVSVCKK
AKIAVLLLPGIGTIRELDAAAKRGVKMVRIATHVTEADISAQHITRAKELGLEAVGFLMMSHMASTEKLVEQAKLMESYG
ADVVYVVDSAGALLPHEVTERIRALKQSLGIEIGFHGHNNLSLAMANTLAAIQEGATRIDGSVRCLGAGAGNTQTEVLVA
VLDRIGLKTGVDLYKMMDLAEEIVAPILQVPQEITRDSLILGYAGVYSSFRLHAERAAKRFGVDSRDILVELGKRKVVGG
QEDMIIDIAAEIASKKTATGAR
>Mature_341_residues
SSERDIWITEVALRDGSHAINHQYTVEQVVSVAQALDEANVPYIEVSHGDGLAGSSLQYGLSRTDEMELIEAAVSVCKKA
KIAVLLLPGIGTIRELDAAAKRGVKMVRIATHVTEADISAQHITRAKELGLEAVGFLMMSHMASTEKLVEQAKLMESYGA
DVVYVVDSAGALLPHEVTERIRALKQSLGIEIGFHGHNNLSLAMANTLAAIQEGATRIDGSVRCLGAGAGNTQTEVLVAV
LDRIGLKTGVDLYKMMDLAEEIVAPILQVPQEITRDSLILGYAGVYSSFRLHAERAAKRFGVDSRDILVELGKRKVVGGQ
EDMIIDIAAEIASKKTATGAR

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=334, Percent_Identity=55.0898203592814, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6320071, Length=314, Percent_Identity=26.1146496815287, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320019, Length=314, Percent_Identity=26.1146496815287, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_GEOTN (A4IT42)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001127241.1
- ProteinModelPortal:   A4IT42
- SMR:   A4IT42
- STRING:   A4IT42
- GeneID:   4968077
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_3151
- NMPDR:   fig|420246.5.peg.3019
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   ELGSDAQ
- ProtClustDB:   PRK08195
- BioCyc:   GTHE420246:GTNG_3151-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36711; Mature: 36580

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 169-169 BINDING 196-196 BINDING 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSERDIWITEVALRDGSHAINHQYTVEQVVSVAQALDEANVPYIEVSHGDGLAGSSLQY
CCCCCCEEEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
GLSRTDEMELIEAAVSVCKKAKIAVLLLPGIGTIRELDAAAKRGVKMVRIATHVTEADIS
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCH
AQHITRAKELGLEAVGFLMMSHMASTEKLVEQAKLMESYGADVVYVVDSAGALLPHEVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
RIRALKQSLGIEIGFHGHNNLSLAMANTLAAIQEGATRIDGSVRCLGAGAGNTQTEVLVA
HHHHHHHHHCEEEEECCCCCEEHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCHHHHHHH
VLDRIGLKTGVDLYKMMDLAEEIVAPILQVPQEITRDSLILGYAGVYSSFRLHAERAAKR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
FGVDSRDILVELGKRKVVGGQEDMIIDIAAEIASKKTATGAR
HCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSERDIWITEVALRDGSHAINHQYTVEQVVSVAQALDEANVPYIEVSHGDGLAGSSLQY
CCCCCEEEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
GLSRTDEMELIEAAVSVCKKAKIAVLLLPGIGTIRELDAAAKRGVKMVRIATHVTEADIS
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEHHHHHHHHHCH
AQHITRAKELGLEAVGFLMMSHMASTEKLVEQAKLMESYGADVVYVVDSAGALLPHEVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
RIRALKQSLGIEIGFHGHNNLSLAMANTLAAIQEGATRIDGSVRCLGAGAGNTQTEVLVA
HHHHHHHHHCEEEEECCCCCEEHHHHHHHHHHHHCHHHCCCCEEEEECCCCCCHHHHHHH
VLDRIGLKTGVDLYKMMDLAEEIVAPILQVPQEITRDSLILGYAGVYSSFRLHAERAAKR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
FGVDSRDILVELGKRKVVGGQEDMIIDIAAEIASKKTATGAR
HCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA