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The map label for this gene is glcD [H]

Identifier: 138896773

GI number: 138896773

Start: 3237584

End: 3238951

Strand: Reverse

Name: glcD [H]

Synonym: GTNG_3136

Alternate gene names: 138896773

Gene position: 3238951-3237584 (Counterclockwise)

Preceding gene: 138896774

Following gene: 138896772

Centisome position: 91.23

GC content: 55.99

Gene sequence:

>1368_bases
ATGAACGTGTACGAATCACTGCTTGAGTTGATCGGCGACCGTGAACGGGTGAGCAGAAACGAGACGGTGCTTGAACACCA
TAGCAAAGGTATCGCCTACCATGAGCCGCACAAACCGGATGTCGTCGTCTTTCCGAAAACGGCCGAGGAAGTGAGCCGGG
TATTAGCGTTTGCGAACGAACACCGCATCCCCGTGACGCCGTTTGGCGCCGGCACGAGCTTAGAAGGGCATATCATTCCG
GTCGAAGGCGGTATTAGCCTCGATGTGACACGGATGAACAACATCATCGACATCCGCCCCAACGATTTTCTCGCCATTGT
CGAGCCAGGGGTGACGCGCTTGCAGCTTAACCAAGCGCTCAAACCGTACGGACTGTTTTTCCCGGTCGACCCGGGGGCAG
ATGCGACGATCGGCGGCATGGCGGCAACGAATGCAAGCGGAACGAACTGTGTAAAATATGGCGTCATGCGGGAACAAGTG
CTCGGGCTCGAAGTGGTGCTTGCCGACGGTTCAGTCATTCAAACCGGTAGCTTGGCGGTGAAATCATCTGCTGGCTATGA
CTTGACCGGTTTGTTCGTCGGATCCGAAGGGACGCTCGGGATTTTCACCCGCCTCATCGTCCGGCTGCACGGCATTCCGG
AGGCGACGACGGCCATCAAAGTCGGTTTTCCGTCGCTTGAGGCGGCAGCGCAAGCGGCGTTTGCCGTGTTAAAAGCTGGC
GTTGGACCAGGGAAAATTGAACTCGTGGATGAGGCGACGGTCGCAGCTGTCAACGCGTACAAAAAGACCGATTATCCGGT
AAAGCCGACGCTGTTTATCGAATTGAGCGGCAGCCGTTCGAGCGTAGATGACGGGGCGGAACTCGTCAAAGAACTGTGCG
AAGCGGAGGGAGCAGTTTCGTTTGTGATCGAAACCGACTCGCTCGCCCGTGCGCAGCTTTGGGAAGCGCGCCATCACGCG
GCGTTCGCCATTCAAGCCGCGTATCCGGGCAAGGCGATGCTCTCGACTGATGTGTGCGTGCCGCTGTCCGAACTGCCCGG
GGCGATTGCAGATACACGTCGGCTTGTTAATGAACACAACATGACTGCCGCTATTTTTGGCCATGTCGGTGACGGCAATT
ACCATACGGTGCTTGCCTTTGACCCAAATGATGCTGAAGAGATGAAGCGAATCGAGCATGTGCACACCCATATCGTTCGC
TACGCTCTGTCTCGCGGTGGTACGTGTACAGGTGAGCACGGCATCGGTATCGGCAAGCGAGCGTTTTTGCGAGAAGAAAA
GGGCGATGCAGTATTATGGATGAAGCGGCTGAAACAATTGTTTGACCCAAACGGCATTCTCAACCCGGGGAAAATCTTTT
TGCCGTAA

Upstream 100 bases:

>100_bases
GACGTTGATGCGCTACTAATCAAAACATGCCGCACGGCGTGCTCGACAATTGAAAGGGGAATGGTGCGGCTGTTGTATCC
CAGTGATAAGGGGGAGAACG

Downstream 100 bases:

>100_bases
CGCTTCGTCCGTATGGCGGCTCGATTGATTAGTTTGATAGCGATATTTCTCAAAAACATAAGTTGGATGATTAAGAGGAA
GCGGAGACCGTCTTTTATGG

Product: glycolate oxidase subunit GlcD

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNVYESLLELIGDRERVSRNETVLEHHSKGIAYHEPHKPDVVVFPKTAEEVSRVLAFANEHRIPVTPFGAGTSLEGHIIP
VEGGISLDVTRMNNIIDIRPNDFLAIVEPGVTRLQLNQALKPYGLFFPVDPGADATIGGMAATNASGTNCVKYGVMREQV
LGLEVVLADGSVIQTGSLAVKSSAGYDLTGLFVGSEGTLGIFTRLIVRLHGIPEATTAIKVGFPSLEAAAQAAFAVLKAG
VGPGKIELVDEATVAAVNAYKKTDYPVKPTLFIELSGSRSSVDDGAELVKELCEAEGAVSFVIETDSLARAQLWEARHHA
AFAIQAAYPGKAMLSTDVCVPLSELPGAIADTRRLVNEHNMTAAIFGHVGDGNYHTVLAFDPNDAEEMKRIEHVHTHIVR
YALSRGGTCTGEHGIGIGKRAFLREEKGDAVLWMKRLKQLFDPNGILNPGKIFLP

Sequences:

>Translated_455_residues
MNVYESLLELIGDRERVSRNETVLEHHSKGIAYHEPHKPDVVVFPKTAEEVSRVLAFANEHRIPVTPFGAGTSLEGHIIP
VEGGISLDVTRMNNIIDIRPNDFLAIVEPGVTRLQLNQALKPYGLFFPVDPGADATIGGMAATNASGTNCVKYGVMREQV
LGLEVVLADGSVIQTGSLAVKSSAGYDLTGLFVGSEGTLGIFTRLIVRLHGIPEATTAIKVGFPSLEAAAQAAFAVLKAG
VGPGKIELVDEATVAAVNAYKKTDYPVKPTLFIELSGSRSSVDDGAELVKELCEAEGAVSFVIETDSLARAQLWEARHHA
AFAIQAAYPGKAMLSTDVCVPLSELPGAIADTRRLVNEHNMTAAIFGHVGDGNYHTVLAFDPNDAEEMKRIEHVHTHIVR
YALSRGGTCTGEHGIGIGKRAFLREEKGDAVLWMKRLKQLFDPNGILNPGKIFLP
>Mature_455_residues
MNVYESLLELIGDRERVSRNETVLEHHSKGIAYHEPHKPDVVVFPKTAEEVSRVLAFANEHRIPVTPFGAGTSLEGHIIP
VEGGISLDVTRMNNIIDIRPNDFLAIVEPGVTRLQLNQALKPYGLFFPVDPGADATIGGMAATNASGTNCVKYGVMREQV
LGLEVVLADGSVIQTGSLAVKSSAGYDLTGLFVGSEGTLGIFTRLIVRLHGIPEATTAIKVGFPSLEAAAQAAFAVLKAG
VGPGKIELVDEATVAAVNAYKKTDYPVKPTLFIELSGSRSSVDDGAELVKELCEAEGAVSFVIETDSLARAQLWEARHHA
AFAIQAAYPGKAMLSTDVCVPLSELPGAIADTRRLVNEHNMTAAIFGHVGDGNYHTVLAFDPNDAEEMKRIEHVHTHIVR
YALSRGGTCTGEHGIGIGKRAFLREEKGDAVLWMKRLKQLFDPNGILNPGKIFLP

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=453, Percent_Identity=46.1368653421634, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI37595754, Length=476, Percent_Identity=43.6974789915966, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI119964728, Length=429, Percent_Identity=31.7016317016317, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4501993, Length=229, Percent_Identity=29.6943231441048, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1789351, Length=455, Percent_Identity=33.4065934065934, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1787977, Length=499, Percent_Identity=28.2565130260521, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994907, Length=483, Percent_Identity=26.5010351966874, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994914, Length=374, Percent_Identity=21.9251336898396, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=452, Percent_Identity=43.3628318584071, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71992381, Length=452, Percent_Identity=42.0353982300885, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI71992392, Length=376, Percent_Identity=44.9468085106383, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17534361, Length=425, Percent_Identity=27.7647058823529, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17556096, Length=232, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320027, Length=468, Percent_Identity=38.034188034188, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6320023, Length=435, Percent_Identity=31.7241379310345, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320764, Length=436, Percent_Identity=28.8990825688073, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI18921117, Length=427, Percent_Identity=30.2107728337237, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24639277, Length=427, Percent_Identity=30.2107728337237, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24639275, Length=427, Percent_Identity=30.2107728337237, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24653753, Length=218, Percent_Identity=28.8990825688073, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 48841; Mature: 48841

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYESLLELIGDRERVSRNETVLEHHSKGIAYHEPHKPDVVVFPKTAEEVSRVLAFANE
CCHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC
HRIPVTPFGAGTSLEGHIIPVEGGISLDVTRMNNIIDIRPNDFLAIVEPGVTRLQLNQAL
CCCCEECCCCCCCCCCEEEEECCCCEEEEEECCCEEEECCCCEEEEECCCCEEEEHHHCC
KPYGLFFPVDPGADATIGGMAATNASGTNCVKYGVMREQVLGLEVVLADGSVIQTGSLAV
CCCEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCEEECCCEEE
KSSAGYDLTGLFVGSEGTLGIFTRLIVRLHGIPEATTAIKVGFPSLEAAAQAAFAVLKAG
ECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
VGPGKIELVDEATVAAVNAYKKTDYPVKPTLFIELSGSRSSVDDGAELVKELCEAEGAVS
CCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
FVIETDSLARAQLWEARHHAAFAIQAAYPGKAMLSTDVCVPLSELPGAIADTRRLVNEHN
EEEECCCHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCHHHCCCHHHHHHHHHHHCC
MTAAIFGHVGDGNYHTVLAFDPNDAEEMKRIEHVHTHIVRYALSRGGTCTGEHGIGIGKR
CEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AFLREEKGDAVLWMKRLKQLFDPNGILNPGKIFLP
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MNVYESLLELIGDRERVSRNETVLEHHSKGIAYHEPHKPDVVVFPKTAEEVSRVLAFANE
CCHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC
HRIPVTPFGAGTSLEGHIIPVEGGISLDVTRMNNIIDIRPNDFLAIVEPGVTRLQLNQAL
CCCCEECCCCCCCCCCEEEEECCCCEEEEEECCCEEEECCCCEEEEECCCCEEEEHHHCC
KPYGLFFPVDPGADATIGGMAATNASGTNCVKYGVMREQVLGLEVVLADGSVIQTGSLAV
CCCEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCEEECCCEEE
KSSAGYDLTGLFVGSEGTLGIFTRLIVRLHGIPEATTAIKVGFPSLEAAAQAAFAVLKAG
ECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
VGPGKIELVDEATVAAVNAYKKTDYPVKPTLFIELSGSRSSVDDGAELVKELCEAEGAVS
CCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
FVIETDSLARAQLWEARHHAAFAIQAAYPGKAMLSTDVCVPLSELPGAIADTRRLVNEHN
EEEECCCHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCHHHCCCHHHHHHHHHHHCC
MTAAIFGHVGDGNYHTVLAFDPNDAEEMKRIEHVHTHIVRYALSRGGTCTGEHGIGIGKR
CEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AFLREEKGDAVLWMKRLKQLFDPNGILNPGKIFLP
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]