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The map label for this gene is ppaX [H]

Identifier: 138896666

GI number: 138896666

Start: 3124703

End: 3125347

Strand: Reverse

Name: ppaX [H]

Synonym: GTNG_3029

Alternate gene names: 138896666

Gene position: 3125347-3124703 (Counterclockwise)

Preceding gene: 138896667

Following gene: 138896665

Centisome position: 88.03

GC content: 50.7

Gene sequence:

>645_bases
ATGACGATTCGTACCATTTTATTTGACCTTGACGGAACGTTGATTGATACAAATGAGCTGATCATCCAGTCGTTTTTGCA
TACGCTCGAGAGGTACTATCCGGGCCGCTATGGGCGTGAGGACGTCCTGCCGTTCATCGGCCCGTCGTTGTATGAGACGT
TCGGATCACTCGATCCAGAACGGATGGAAGAAATGGTCAAGACTTACCGCACGTTTAACCATGCCCGTCATGATGAGTTG
ATTCGCGAGTTTGACACGGTGTATGAGACGATCGAAACGCTTCACCGCCATGGCTTCCGCCTTGGCGTGGTGACGACGAA
AATTCATGACACAGCGCTCATGGGCTTGCGGAAAACGCGTTTAGAGCCGTTTTTCTCGTGCGTCATCGGTCTCGATGACG
TTAGTCGTCCAAAACCGGATCCGGAACCAATTTATAAAGCGCTTGATGTGCTTCGGTCGACACCGAACGAAGCGCTCATG
GTCGGCGACAACTACCACGACATCTTAGCCGGCAAAAACGCCGGTGTAAAAACAGCCGGCGTGGCGTGGTCGATTAAAGG
GCGCAAGTATTTGGAACAATATGAACCGGACTTTATGCTGGAAAAAATGAGCGATTTGTTGGCCATTGTCGGCATGAACG
AATGA

Upstream 100 bases:

>100_bases
CGCTGCTTGTGATCCGCCTAGTGACGGCCGTATTGCTGACGATGGCGGTGGCGTTCATTTGGCGCCGCCTGGAACAACCG
AAAAGAAAGGAAGCCGCTTC

Downstream 100 bases:

>100_bases
AAGGAGGAACCGTCCTCCCAGTGAGACGAACGACGAAATACCCCGTGCAAGGGGCGAACTCACTCTGGCAATTGTACCGC
ACCGTATCGTTTTGGAAAGT

Product: pyrophosphatase PpaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTIRTILFDLDGTLIDTNELIIQSFLHTLERYYPGRYGREDVLPFIGPSLYETFGSLDPERMEEMVKTYRTFNHARHDEL
IREFDTVYETIETLHRHGFRLGVVTTKIHDTALMGLRKTRLEPFFSCVIGLDDVSRPKPDPEPIYKALDVLRSTPNEALM
VGDNYHDILAGKNAGVKTAGVAWSIKGRKYLEQYEPDFMLEKMSDLLAIVGMNE

Sequences:

>Translated_214_residues
MTIRTILFDLDGTLIDTNELIIQSFLHTLERYYPGRYGREDVLPFIGPSLYETFGSLDPERMEEMVKTYRTFNHARHDEL
IREFDTVYETIETLHRHGFRLGVVTTKIHDTALMGLRKTRLEPFFSCVIGLDDVSRPKPDPEPIYKALDVLRSTPNEALM
VGDNYHDILAGKNAGVKTAGVAWSIKGRKYLEQYEPDFMLEKMSDLLAIVGMNE
>Mature_213_residues
TIRTILFDLDGTLIDTNELIIQSFLHTLERYYPGRYGREDVLPFIGPSLYETFGSLDPERMEEMVKTYRTFNHARHDELI
REFDTVYETIETLHRHGFRLGVVTTKIHDTALMGLRKTRLEPFFSCVIGLDDVSRPKPDPEPIYKALDVLRSTPNEALMV
GDNYHDILAGKNAGVKTAGVAWSIKGRKYLEQYEPDFMLEKMSDLLAIVGMNE

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=235, Percent_Identity=28.0851063829787, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24621; Mature: 24489

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRTILFDLDGTLIDTNELIIQSFLHTLERYYPGRYGREDVLPFIGPSLYETFGSLDPE
CCCEEEEEECCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHCCCCHH
RMEEMVKTYRTFNHARHDELIREFDTVYETIETLHRHGFRLGVVTTKIHDTALMGLRKTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
LEPFFSCVIGLDDVSRPKPDPEPIYKALDVLRSTPNEALMVGDNYHDILAGKNAGVKTAG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEECC
VAWSIKGRKYLEQYEPDFMLEKMSDLLAIVGMNE
EEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIRTILFDLDGTLIDTNELIIQSFLHTLERYYPGRYGREDVLPFIGPSLYETFGSLDPE
CCEEEEEECCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHCCCCHH
RMEEMVKTYRTFNHARHDELIREFDTVYETIETLHRHGFRLGVVTTKIHDTALMGLRKTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
LEPFFSCVIGLDDVSRPKPDPEPIYKALDVLRSTPNEALMVGDNYHDILAGKNAGVKTAG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCEECC
VAWSIKGRKYLEQYEPDFMLEKMSDLLAIVGMNE
EEEEECCHHHHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA