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The map label for this gene is cheY [H]

Identifier: 138896477

GI number: 138896477

Start: 2953510

End: 2953872

Strand: Direct

Name: cheY [H]

Synonym: GTNG_2840

Alternate gene names: 138896477

Gene position: 2953510-2953872 (Clockwise)

Preceding gene: 138896441

Following gene: 138896497

Centisome position: 83.19

GC content: 36.91

Gene sequence:

>363_bases
ATGCCAAAAGCAACAGTCCTTATTGCTGATGATTCCATGTTTATGAGGAACTTAATCAAAGATCTTCTGTCTCGTCATGG
TTATCAGATTATAGCTGAGGCGTCTGATGGTTACAATGCCGTAGCTCTTTACGGAGAAAAACATCCTGACATTGCGATTT
TAGACCTTATTATGCCTAAGATGAATGGCCTGCATGCCCTAAAGCAAATCCTACTAATCGATCCCAACGCTAAAGTGATT
ATGTGTTCCTCAATGGGGCAAAAATATCTTACCATTGAGGCACTTCAAATTGGAGCCAAAGATTTTATTGTTAAACCTTA
TATAAATGAATTAGTTCTCTCACTAAACAAATTATGTAGTTAA

Upstream 100 bases:

>100_bases
TAGTTCCATTTTCCTGTGTTAATTTAACTATTAAATTGCTAATATGAATTTTGTGTATCGACAATATTCGACAATTCGTC
AAAATAGATGGAGGTGTACA

Downstream 100 bases:

>100_bases
TCATGATTATAGATACCCCTCGTCCACTCTGAATTGTCTAGATTTTTCCCCTCTTCCTTTCTTCTATTTTCTCTATGAAT
GTACTTCGCGTACAACAACA

Product: chemotaxis response regulator CheY

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 119

Protein sequence:

>120_residues
MPKATVLIADDSMFMRNLIKDLLSRHGYQIIAEASDGYNAVALYGEKHPDIAILDLIMPKMNGLHALKQILLIDPNAKVI
MCSSMGQKYLTIEALQIGAKDFIVKPYINELVLSLNKLCS

Sequences:

>Translated_120_residues
MPKATVLIADDSMFMRNLIKDLLSRHGYQIIAEASDGYNAVALYGEKHPDIAILDLIMPKMNGLHALKQILLIDPNAKVI
MCSSMGQKYLTIEALQIGAKDFIVKPYINELVLSLNKLCS
>Mature_119_residues
PKATVLIADDSMFMRNLIKDLLSRHGYQIIAEASDGYNAVALYGEKHPDIAILDLIMPKMNGLHALKQILLIDPNAKVIM
CSSMGQKYLTIEALQIGAKDFIVKPYINELVLSLNKLCS

Specific function: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheY seems to regulate the clockwise (CW) rotation [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=123, Percent_Identity=35.7723577235772, Blast_Score=69, Evalue=7e-14,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=30.0884955752212, Blast_Score=69, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 13318; Mature: 13187

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKATVLIADDSMFMRNLIKDLLSRHGYQIIAEASDGYNAVALYGEKHPDIAILDLIMPK
CCCEEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCCEEEEEHHHCC
MNGLHALKQILLIDPNAKVIMCSSMGQKYLTIEALQIGAKDFIVKPYINELVLSLNKLCS
CCHHHHHHHHEEECCCCCEEEECCCCCEEEEEEHEEECCCCEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKATVLIADDSMFMRNLIKDLLSRHGYQIIAEASDGYNAVALYGEKHPDIAILDLIMPK
CCEEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCCEEEEEHHHCC
MNGLHALKQILLIDPNAKVIMCSSMGQKYLTIEALQIGAKDFIVKPYINELVLSLNKLCS
CCHHHHHHHHEEECCCCCEEEECCCCCEEEEEEHEEECCCCEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550470; 10360571; 9521117 [H]