Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 138896388

GI number: 138896388

Start: 2869538

End: 2871124

Strand: Direct

Name: pckA [H]

Synonym: GTNG_2751

Alternate gene names: 138896388

Gene position: 2869538-2871124 (Clockwise)

Preceding gene: 138896386

Following gene: 138896391

Centisome position: 80.82

GC content: 54.38

Gene sequence:

>1587_bases
ATGGGGATCGCAAAAATGACCAATAAGCTTTCTTTGCTGCTGCAAAAACCGTACGTACATCACCAGTTGTCCGTTGCTGA
GCTCGTCGAAAAAGTGCTGCACCGAAACGAAGGGCGGCTGACGCACACTGGCGCTGTCGCCGTCACGACCGGTAAATATA
CCGGACGGTCACCGAAAGACAAATACATCGTTGAAGAACCGTCGACGAAACAGAACATCGATTGGGGAACGGTCAACCAA
CCAATGGCACCGGAGACATTTCAAAAATTATATGATAAAGTGCTCGATTATTTAATGAAAAAAGATGAACTGTTCGTCTT
TAAAGGCTTCGCCGGCGCTGACCCGAAAGCGCGGCTGCCGATTCAAGTGGTGAACGAATTCGCTTGGCACAACTTGTTTG
TCCATCAGCTGTTCATCCGTCCAAGCGCGGAAGAACTCGCCGACCATGAACCGCAATTCACCGTCATTTGCGCGCCGAAC
TTTAAAGCAGACCCGGCCGTTGACGGGACGCGTTCGGAAGCGTTTATCATCATCTCGTTCGAACAGCGCACCGTCTTGAT
CGGTGGAACGGAATATGCGGGCGAAATGAAAAAATCGATCTTCTCGGTCATGAACTATTTGCTGCCGGAACAAGGCATCT
TGCCGATGCACTGCTCGGCCAACGTCGGTCAGGAAGGCGACGTCGCCCTCTTCTTCGGCCTGTCGGGAACCGGGAAAACA
ACGCTCTCGACCGACCCGAAACGCCGCTTGATCGGCGATGACGAACACGGCTGGTCGAGCCGCGGCATTTTCAACATCGA
AGGCGGCTGCTATGCGAAATGCATCAACTTATCGCGCGAGAAAGAGCCGCAAATTTTTGATGCCATCGGCTTTGGCGCCG
TGCTTGAAAACGTCATTCTCCATGATGCGACACGGGTTCCGGATTACGATGACGGGACATTGACGGAAAACACGCGCGCG
GCCTATCCGCTTCAGGCGATCAAAAACATCGTTGACCCAAGCGTTGCCGGCCATCCGTCGACCATCGTGTTCTTGACGGC
CGATGCGTTTGGCGTCTTGCCGCCGATCAGCAAGCTGACGCGCGAACAGGCGATGTACCATTTCTTAAGCGGCTACACAA
GCAAACTCGCCGGCACCGAACGCGGCGTCACTGAACCGGAAGCGACGTTCTCGACATGCTTTGGCGCGCCGTTTTTGCCG
CGCCCGGCCGTCGAATACGCCGAAATGCTCGGGCAAAAAATCGCTGAGCATAACGTCCGTGTCTTTTTGGTCAACACTGG
TTGGACAGGAGGACCATACGGCGTCGGCAGCCGAATGAAGCTCGCCTACACCCGCGCGATGGTACAAGCCGCCGTCGAAG
GGGAGCTTGACAACGTCGAAACGGTCCAAGACCCGATTTTCGGTCTTGCCATCCCTGCCCACGTCCCGGGCGTGCCAGAT
GACGTCTTGCGTCCGCAAAACACATGGGCAGACAAACAAGCGTACGAACAAAAAGCGAAAGAACTGGCGGAAAAATTCCG
CGCTAACTTCCAAAAATTCGCTCACATCGATCCGTCCATTGAAAAACTCGGTGGACCGCTCGTTTAA

Upstream 100 bases:

>100_bases
AACTAAGTTTAAAAAACCTACACAATTAGTATGGACTATTAATAAAAATGTGTTATACTATTTTCAAGAGGAATTCCAAT
AAAAAAGGCAGGTTGTGAAT

Downstream 100 bases:

>100_bases
CAAACACCCGGCCGGACAGGGAACATCCTGTCCGGCCGTTTTTTCTGTTCTTTCCTCTATTTCGGTCTCCGTGATTCTCT
TTCTCTTTGACAAGCAGGGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MGIAKMTNKLSLLLQKPYVHHQLSVAELVEKVLHRNEGRLTHTGAVAVTTGKYTGRSPKDKYIVEEPSTKQNIDWGTVNQ
PMAPETFQKLYDKVLDYLMKKDELFVFKGFAGADPKARLPIQVVNEFAWHNLFVHQLFIRPSAEELADHEPQFTVICAPN
FKADPAVDGTRSEAFIIISFEQRTVLIGGTEYAGEMKKSIFSVMNYLLPEQGILPMHCSANVGQEGDVALFFGLSGTGKT
TLSTDPKRRLIGDDEHGWSSRGIFNIEGGCYAKCINLSREKEPQIFDAIGFGAVLENVILHDATRVPDYDDGTLTENTRA
AYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLTREQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLP
RPAVEYAEMLGQKIAEHNVRVFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIPAHVPGVPD
DVLRPQNTWADKQAYEQKAKELAEKFRANFQKFAHIDPSIEKLGGPLV

Sequences:

>Translated_528_residues
MGIAKMTNKLSLLLQKPYVHHQLSVAELVEKVLHRNEGRLTHTGAVAVTTGKYTGRSPKDKYIVEEPSTKQNIDWGTVNQ
PMAPETFQKLYDKVLDYLMKKDELFVFKGFAGADPKARLPIQVVNEFAWHNLFVHQLFIRPSAEELADHEPQFTVICAPN
FKADPAVDGTRSEAFIIISFEQRTVLIGGTEYAGEMKKSIFSVMNYLLPEQGILPMHCSANVGQEGDVALFFGLSGTGKT
TLSTDPKRRLIGDDEHGWSSRGIFNIEGGCYAKCINLSREKEPQIFDAIGFGAVLENVILHDATRVPDYDDGTLTENTRA
AYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLTREQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLP
RPAVEYAEMLGQKIAEHNVRVFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIPAHVPGVPD
DVLRPQNTWADKQAYEQKAKELAEKFRANFQKFAHIDPSIEKLGGPLV
>Mature_527_residues
GIAKMTNKLSLLLQKPYVHHQLSVAELVEKVLHRNEGRLTHTGAVAVTTGKYTGRSPKDKYIVEEPSTKQNIDWGTVNQP
MAPETFQKLYDKVLDYLMKKDELFVFKGFAGADPKARLPIQVVNEFAWHNLFVHQLFIRPSAEELADHEPQFTVICAPNF
KADPAVDGTRSEAFIIISFEQRTVLIGGTEYAGEMKKSIFSVMNYLLPEQGILPMHCSANVGQEGDVALFFGLSGTGKTT
LSTDPKRRLIGDDEHGWSSRGIFNIEGGCYAKCINLSREKEPQIFDAIGFGAVLENVILHDATRVPDYDDGTLTENTRAA
YPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLTREQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLPR
PAVEYAEMLGQKIAEHNVRVFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIPAHVPGVPDD
VLRPQNTWADKQAYEQKAKELAEKFRANFQKFAHIDPSIEKLGGPLV

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=499, Percent_Identity=52.5050100200401, Blast_Score=508, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6322950, Length=515, Percent_Identity=50.4854368932039, Blast_Score=502, Evalue=1e-143,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58259; Mature: 58128

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAKMTNKLSLLLQKPYVHHQLSVAELVEKVLHRNEGRLTHTGAVAVTTGKYTGRSPKD
CCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCC
KYIVEEPSTKQNIDWGTVNQPMAPETFQKLYDKVLDYLMKKDELFVFKGFAGADPKARLP
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCH
IQVVNEFAWHNLFVHQLFIRPSAEELADHEPQFTVICAPNFKADPAVDGTRSEAFIIISF
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EQRTVLIGGTEYAGEMKKSIFSVMNYLLPEQGILPMHCSANVGQEGDVALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCC
TLSTDPKRRLIGDDEHGWSSRGIFNIEGGCYAKCINLSREKEPQIFDAIGFGAVLENVIL
CCCCCCHHHCCCCCCCCCCCCCEEEECCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDATRVPDYDDGTLTENTRAAYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLT
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHH
REQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLPRPAVEYAEMLGQKIAEHNVR
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIPAHVPGVPD
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHEEECCCCCCCCCH
DVLRPQNTWADKQAYEQKAKELAEKFRANFQKFAHIDPSIEKLGGPLV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
>Mature Secondary Structure 
GIAKMTNKLSLLLQKPYVHHQLSVAELVEKVLHRNEGRLTHTGAVAVTTGKYTGRSPKD
CHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCC
KYIVEEPSTKQNIDWGTVNQPMAPETFQKLYDKVLDYLMKKDELFVFKGFAGADPKARLP
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCH
IQVVNEFAWHNLFVHQLFIRPSAEELADHEPQFTVICAPNFKADPAVDGTRSEAFIIISF
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EQRTVLIGGTEYAGEMKKSIFSVMNYLLPEQGILPMHCSANVGQEGDVALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCC
TLSTDPKRRLIGDDEHGWSSRGIFNIEGGCYAKCINLSREKEPQIFDAIGFGAVLENVIL
CCCCCCHHHCCCCCCCCCCCCCEEEECCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDATRVPDYDDGTLTENTRAAYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLT
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHH
REQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLPRPAVEYAEMLGQKIAEHNVR
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIPAHVPGVPD
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHEEECCCCCCCCCH
DVLRPQNTWADKQAYEQKAKELAEKFRANFQKFAHIDPSIEKLGGPLV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA