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The map label for this gene is trmB

Identifier: 138896365

GI number: 138896365

Start: 2845216

End: 2845866

Strand: Reverse

Name: trmB

Synonym: GTNG_2728

Alternate gene names: 138896365

Gene position: 2845866-2845216 (Counterclockwise)

Preceding gene: 138896367

Following gene: 138896364

Centisome position: 80.16

GC content: 50.84

Gene sequence:

>651_bases
ATGCGTTTGCGCAACAAACCATGGGCGAAAGACAAAATCGCCGCTTACCCTCAGTATGTCATCCCTGACCCGGAAGCGAG
GCGCGGGCGTTGGCATGAGCTGTTTGGCAATGACCGGCCGATCCATATTGAAATCGGCACTGGAAAAGGAAAATTTATTA
CCGAAATGGCGAAGCTGCATCCCGACGTCAATTTTATCGGCATTGAGTTGTATCCGAGCGTGCTCGTGTCGGCGCTGGAT
AAGCTGATCGAGAGCGAGCTGCCAAACGTGCGGTTGCTCAATGCCAACGCGAAAGATCTCACCTCATTTTTTGCTGACGG
AGAAATCGCGCGTATTTACTTGAACTTTTCCGACCCGTGGCCGAAAAAGCGGCATGAGAAACGGCGGCTGACCTATCGGG
ATTTTTTGGCGCTTTATGATCGCATTTTGTCCGCGGACGGGGACATTCATTTAAAAACGGACAATCAGTCGTTTTTTGAG
TATTCGCTTGTCAGCCTGTCACAATATGGGTTTGTGCTCGCGGCGGTCCAATTGGATTTGCATCGGAGCGATATTGTCGG
CAACGTCATGACCGAGTACGAAGAAAAATTTTCAGCAAAAGGCAACCGCATTTACCGCTGCGAGGCGCTTCGTCCGCCAC
AGCAGTCATAA

Upstream 100 bases:

>100_bases
GCCTAACGGTGTTTATGCCTAGGCGTTTGCTTCTTCAAGCGCGGTTATACTAACAAGGTAGCATCAAAACAACAAACGGA
AACGAGACAGAGGTGTCAAT

Downstream 100 bases:

>100_bases
CGGCCCGACCGGTTTGGTCGGGTTTTTTTAGTGCTCTGAACCGGTCTCACCACGTTTCCTCGTGAGCATAAGTGGAGGAT
GCATCCGTGCCCTCACCAAC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLHPDVNFIGIELYPSVLVSALD
KLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPWPKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFE
YSLVSLSQYGFVLAAVQLDLHRSDIVGNVMTEYEEKFSAKGNRIYRCEALRPPQQS

Sequences:

>Translated_216_residues
MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLHPDVNFIGIELYPSVLVSALD
KLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPWPKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFE
YSLVSLSQYGFVLAAVQLDLHRSDIVGNVMTEYEEKFSAKGNRIYRCEALRPPQQS
>Mature_216_residues
MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLHPDVNFIGIELYPSVLVSALD
KLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPWPKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFE
YSLVSLSQYGFVLAAVQLDLHRSDIVGNVMTEYEEKFSAKGNRIYRCEALRPPQQS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=135, Percent_Identity=34.0740740740741, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_GEOTN (A4IRW9)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126818.1
- ProteinModelPortal:   A4IRW9
- SMR:   A4IRW9
- STRING:   A4IRW9
- GeneID:   4967654
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2728
- NMPDR:   fig|420246.5.peg.2632
- eggNOG:   COG0220
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- PhylomeDB:   A4IRW9
- ProtClustDB:   PRK00121
- BioCyc:   GTHE420246:GTNG_2728-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25001; Mature: 25001

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLH
CCCCCCCCCCCHHHCCCCEECCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHC
PDVNFIGIELYPSVLVSALDKLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPW
CCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCC
PKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFEYSLVSLSQYGFVLAAVQLDL
CHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEHHH
HRSDIVGNVMTEYEEKFSAKGNRIYRCEALRPPQQS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLH
CCCCCCCCCCCHHHCCCCEECCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHC
PDVNFIGIELYPSVLVSALDKLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPW
CCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCC
PKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFEYSLVSLSQYGFVLAAVQLDL
CHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEHHH
HRSDIVGNVMTEYEEKFSAKGNRIYRCEALRPPQQS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA