BacMap

Definition	Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession	NC_009328
Length	3,550,319

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The map label for this gene is fabG [H]

Identifier: 138896315

GI number: 138896315

Start: 2788774

End: 2789538

Strand: Reverse

Name: fabG [H]

Synonym: GTNG_2678

Alternate gene names: 138896315

Gene position: 2789538-2788774 (Counterclockwise)

Preceding gene: 138896316

Following gene: 138896314

Centisome position: 78.57

GC content: 54.9

Gene sequence:

>765_bases
GTGCGGCACGCCTTAATCACGGCTGGAGCGAAAGGGCTGGGTCGAAAAGTGACGGAGCTGCTGCTGGACAAAGGTTATTC
AGTGACGGTTAACTACCGGAGCGATGAACGGGCAGTGCACTTGCTTCAAGAACGGTATAAGGACGCGAGCGATCGTCTCC
AATTTGTGCGCGGCGATGTGACCAACAAAGACGATTTGGCGGCATTGGTTGACGCGGCCATGGAACGGTTCGGCCGCATT
GATTTCCTCATTAACAACGCGGGACCATACATTTTTGAACGGAAAAAGCTGGCCGATTATACGGAAGATGAATGGTACGA
GATGGTGGAAGGCAACTTGAGCTCGGTGTTCCATTTGGTAAGGCGGACGATTCCGATTATGCGGAAACAACGGTTCGGTC
GCATCATTACGTATGGGTTCCAAGGAGCGGCCGATGCCCCGGGATGGGTGCATCGTTCGGCCTTTGGCGCTGCGAAGGTC
GGGCTTGTGTCATTGACGAAAACGATCGCTCTCGAAGAGGCGGAATACGGCATCACCGCCAACATGGTCTGTCCAGGCAA
CATCGTTGGCGACATGAAAGAAGCGGACATTCAAGATGCCCGCACCAAGCGTGATGAGGAAACGCCCATCGGACGGCCTG
GGACGGGAGAGGATATCGCTCGTGTCATCGCCTTTTTGTGCGAAGACGATTCGGACTTCATCACCGGCGCTGTCATTGAT
GTCACCGGCGGAGCGAACGTCATCTATCGCCACTTTTTCCGGTGA

Upstream 100 bases:

>100_bases
CCTCTCTAAGCGTAAACGTCGGTGGCAGAGCGTTCCAATTCTCTCCTGTTTGTAGTAAAGTAAAAATGAGACAGGCGAAT
AGCGGCAAGGAGGGAGAACA

Downstream 100 bases:

>100_bases
TCGCAATGCCGTCTTCTTTTGCTGTTTGCTTTCCGGTTGAACAGGGAACAATAAAAGAGGAAGTGGCAAGGAAAAAGGAG
GAAAATGAACGGTGAAAATT

Product: 3-ketoacyl-(acyl-carrier-protein) reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRHALITAGAKGLGRKVTELLLDKGYSVTVNYRSDERAVHLLQERYKDASDRLQFVRGDVTNKDDLAALVDAAMERFGRI
DFLINNAGPYIFERKKLADYTEDEWYEMVEGNLSSVFHLVRRTIPIMRKQRFGRIITYGFQGAADAPGWVHRSAFGAAKV
GLVSLTKTIALEEAEYGITANMVCPGNIVGDMKEADIQDARTKRDEETPIGRPGTGEDIARVIAFLCEDDSDFITGAVID
VTGGANVIYRHFFR

Sequences:

>Translated_254_residues
MRHALITAGAKGLGRKVTELLLDKGYSVTVNYRSDERAVHLLQERYKDASDRLQFVRGDVTNKDDLAALVDAAMERFGRI
DFLINNAGPYIFERKKLADYTEDEWYEMVEGNLSSVFHLVRRTIPIMRKQRFGRIITYGFQGAADAPGWVHRSAFGAAKV
GLVSLTKTIALEEAEYGITANMVCPGNIVGDMKEADIQDARTKRDEETPIGRPGTGEDIARVIAFLCEDDSDFITGAVID
VTGGANVIYRHFFR
>Mature_254_residues
MRHALITAGAKGLGRKVTELLLDKGYSVTVNYRSDERAVHLLQERYKDASDRLQFVRGDVTNKDDLAALVDAAMERFGRI
DFLINNAGPYIFERKKLADYTEDEWYEMVEGNLSSVFHLVRRTIPIMRKQRFGRIITYGFQGAADAPGWVHRSAFGAAKV
GLVSLTKTIALEEAEYGITANMVCPGNIVGDMKEADIQDARTKRDEETPIGRPGTGEDIARVIAFLCEDDSDFITGAVID
VTGGANVIYRHFFR

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=266, Percent_Identity=28.9473684210526, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI59889578, Length=201, Percent_Identity=30.8457711442786, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=29.3172690763052, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.2040816326531, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI66933014, Length=192, Percent_Identity=28.6458333333333, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=27.9352226720648, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=28.2352941176471, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4503301, Length=203, Percent_Identity=27.0935960591133, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4504505, Length=193, Percent_Identity=29.5336787564767, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4758504, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=241, Percent_Identity=34.4398340248963, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788459, Length=242, Percent_Identity=29.7520661157025, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=29.1497975708502, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=26.8, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=26.8, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=26.3779527559055, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789208, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=27.843137254902, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=27.34375, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789378, Length=249, Percent_Identity=24.8995983935743, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=259, Percent_Identity=31.6602316602317, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17561402, Length=248, Percent_Identity=29.8387096774194, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=29.3436293436293, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=250, Percent_Identity=29.2, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=29.4339622641509, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17563726, Length=260, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=27.7566539923954, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=262, Percent_Identity=27.8625954198473, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=29.3893129770992, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=26.7489711934156, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=27.2030651340996, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17562908, Length=260, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=266, Percent_Identity=27.8195488721804, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=261, Percent_Identity=29.1187739463602, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=264, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=26.9961977186312, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=262, Percent_Identity=26.3358778625954, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=24.6212121212121, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=256, Percent_Identity=25.78125, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=23.7547892720307, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=26.5384615384615, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=189, Percent_Identity=29.1005291005291, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=200, Percent_Identity=30.5, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=259, Percent_Identity=26.2548262548263, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=243, Percent_Identity=24.6913580246914, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=192, Percent_Identity=32.2916666666667, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=27.4900398406374, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=201, Percent_Identity=24.8756218905473, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322226, Length=194, Percent_Identity=30.4123711340206, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24644339, Length=246, Percent_Identity=26.8292682926829, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355319, Length=245, Percent_Identity=26.530612244898, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357041, Length=246, Percent_Identity=26.4227642276423, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18859837, Length=249, Percent_Identity=29.718875502008, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI23397609, Length=246, Percent_Identity=27.2357723577236, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28571526, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=27.0491803278689, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17737361, Length=251, Percent_Identity=26.2948207171315, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28358; Mature: 28358

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHALITAGAKGLGRKVTELLLDKGYSVTVNYRSDERAVHLLQERYKDASDRLQFVRGDV
CCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCC
TNKDDLAALVDAAMERFGRIDFLINNAGPYIFERKKLADYTEDEWYEMVEGNLSSVFHLV
CCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHCCHHHHHHHH
RRTIPIMRKQRFGRIITYGFQGAADAPGWVHRSAFGAAKVGLVSLTKTIALEEAEYGITA
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEE
NMVCPGNIVGDMKEADIQDARTKRDEETPIGRPGTGEDIARVIAFLCEDDSDFITGAVID
EEECCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEE
VTGGANVIYRHFFR
ECCCHHHHHHHHCC
>Mature Secondary Structure
MRHALITAGAKGLGRKVTELLLDKGYSVTVNYRSDERAVHLLQERYKDASDRLQFVRGDV
CCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCC
TNKDDLAALVDAAMERFGRIDFLINNAGPYIFERKKLADYTEDEWYEMVEGNLSSVFHLV
CCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHCCCCHHHHHHHHHCCHHHHHHHH
RRTIPIMRKQRFGRIITYGFQGAADAPGWVHRSAFGAAKVGLVSLTKTIALEEAEYGITA
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEE
NMVCPGNIVGDMKEADIQDARTKRDEETPIGRPGTGEDIARVIAFLCEDDSDFITGAVID
EEECCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEE
VTGGANVIYRHFFR
ECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]