Definition Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession NC_009328
Length 3,550,319

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The map label for this gene is fadB [H]

Identifier: 138896252

GI number: 138896252

Start: 2722373

End: 2723146

Strand: Reverse

Name: fadB [H]

Synonym: GTNG_2615

Alternate gene names: 138896252

Gene position: 2723146-2722373 (Counterclockwise)

Preceding gene: 138896253

Following gene: 138896251

Centisome position: 76.7

GC content: 55.68

Gene sequence:

>774_bases
ATGGAGCATTTTCGCTTGACAAAAGAGGAATTCGTCGCTGTCGTGACGTTTTCGCGGCCGCCGGCGAATGCTCTTTCATC
TGCTGTTTTGAAAGAGCTTTCAAATATCTTGGATGAGCTCGAGGCCGACTCGGACGTGCGTGTCGTGCTGTTGCATGGTG
AAGGCCGGTTTTTCTCGGCTGGCGCGGATATTAAAGAGTTTACGTCCGTCGCTTCTGCCGAGGAAGCGTCCGCTTTGTCG
CGCAACGGCCAGCTGGTTGTAGAGCGGATTGAGCGGTTTTCCAAGCCGGTGATTGCTGCTATTCATGGCGCCGCGTTGGG
TGGAGGACTTGAGTTGGCGATGAGCTACCGTATTCGTTTGGTGACAGAAAATGCGAAGCTTGGCCTGCCAGAGCTGCAGC
TTGGCATCATTCCAGGGTTTGCCGGCACGCAGCGGCTCGTGCGCTACGTTGGGTTCGGCAAGGCGGCGGAAATGATGTGG
ACAGGCGAGCCGATCACCGGCTTAGAGGCTGTGCAGTGGGGGCTGGCCAACAAGGCGGTGCCTGAAGAGCGGCTTCTTGA
TGAAGCGAAACAGTTAGCGCACAAAATCGCTGCAAAAAGCCCGCTTTCCGTTCGCGCCACGATTGAGCTTCTCAATGCGG
CGAAAGAAAAATCGTTCGCTGAGGCGGTGCGCGACGAAGCGGAATGGTTTGGCCGTGTGTTTGTTTCCGAGGATGGGAGA
GAAGGTGTGCAGGCGTTTTTAGAAAAACGTCCCCCCGTCTTTCAAGGGAAATAA

Upstream 100 bases:

>100_bases
AAAAATACGACTTGGCTGCGCTCGCCGAGCCAGTGTACGAATTGCTTGCCAAAGGGTGCGCTGCTGGGCGTTGATGGAAG
GCAGAAAGGGGAGAGGCATC

Downstream 100 bases:

>100_bases
TCGCTATAAATGTCGGAATCTTGGCAACTGAAATAATGTTGGATCAAACTGAGGAGGGGAATGACATGAACATTTTTGTT
TTGATGAAACGCACGTTCGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEHFRLTKEEFVAVVTFSRPPANALSSAVLKELSNILDELEADSDVRVVLLHGEGRFFSAGADIKEFTSVASAEEASALS
RNGQLVVERIERFSKPVIAAIHGAALGGGLELAMSYRIRLVTENAKLGLPELQLGIIPGFAGTQRLVRYVGFGKAAEMMW
TGEPITGLEAVQWGLANKAVPEERLLDEAKQLAHKIAAKSPLSVRATIELLNAAKEKSFAEAVRDEAEWFGRVFVSEDGR
EGVQAFLEKRPPVFQGK

Sequences:

>Translated_257_residues
MEHFRLTKEEFVAVVTFSRPPANALSSAVLKELSNILDELEADSDVRVVLLHGEGRFFSAGADIKEFTSVASAEEASALS
RNGQLVVERIERFSKPVIAAIHGAALGGGLELAMSYRIRLVTENAKLGLPELQLGIIPGFAGTQRLVRYVGFGKAAEMMW
TGEPITGLEAVQWGLANKAVPEERLLDEAKQLAHKIAAKSPLSVRATIELLNAAKEKSFAEAVRDEAEWFGRVFVSEDGR
EGVQAFLEKRPPVFQGK
>Mature_257_residues
MEHFRLTKEEFVAVVTFSRPPANALSSAVLKELSNILDELEADSDVRVVLLHGEGRFFSAGADIKEFTSVASAEEASALS
RNGQLVVERIERFSKPVIAAIHGAALGGGLELAMSYRIRLVTENAKLGLPELQLGIIPGFAGTQRLVRYVGFGKAAEMMW
TGEPITGLEAVQWGLANKAVPEERLLDEAKQLAHKIAAKSPLSVRATIELLNAAKEKSFAEAVRDEAEWFGRVFVSEDGR
EGVQAFLEKRPPVFQGK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=39.3574297188755, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI20127408, Length=196, Percent_Identity=38.265306122449, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=35.7843137254902, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=240, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=29.277566539924, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=35.5648535564854, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=25.5506607929515, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=25.5506607929515, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI62530384, Length=181, Percent_Identity=33.1491712707182, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=26.1306532663317, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI37594471, Length=200, Percent_Identity=28, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=204, Percent_Identity=27.9411764705882, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=22.4409448818898, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=22.4409448818898, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=253, Percent_Identity=22.5296442687747, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=231, Percent_Identity=22.9437229437229, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=231, Percent_Identity=22.9437229437229, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=231, Percent_Identity=22.9437229437229, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI25453481, Length=248, Percent_Identity=23.3870967741935, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538573, Length=248, Percent_Identity=23.3870967741935, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=39.1472868217054, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=34.3511450381679, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=41.3793103448276, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=31.7829457364341, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=35.6756756756757, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=31.4393939393939, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=28.2258064516129, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=40.9836065573771, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=38.5826771653543, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=34.9137931034483, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=29.8039215686275, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=37.0165745856354, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17508951, Length=214, Percent_Identity=35.981308411215, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=214, Percent_Identity=35.981308411215, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=214, Percent_Identity=35.981308411215, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=228, Percent_Identity=30.7017543859649, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=27.4436090225564, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=29.8755186721992, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=31.0526315789474, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=31.0526315789474, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=229, Percent_Identity=26.2008733624454, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=21.484375, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=235, Percent_Identity=25.9574468085106, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=27.319587628866, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=43.0327868852459, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=43.0327868852459, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=35.3174603174603, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=31.2741312741313, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583077, Length=181, Percent_Identity=37.0165745856354, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24583079, Length=181, Percent_Identity=37.0165745856354, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921000, Length=181, Percent_Identity=37.0165745856354, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=30.8300395256917, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650670, Length=245, Percent_Identity=29.7959183673469, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583165, Length=239, Percent_Identity=26.7782426778243, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28000; Mature: 28000

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MEHFRLTKEEFVAVVTFSRPPANALSSAVLKELSNILDELEADSDVRVVLLHGEGRFFSA
CCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEC
GADIKEFTSVASAEEASALSRNGQLVVERIERFSKPVIAAIHGAALGGGLELAMSYRIRL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEEEEEEE
VTENAKLGLPELQLGIIPGFAGTQRLVRYVGFGKAAEMMWTGEPITGLEAVQWGLANKAV
EECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHCCCCC
PEERLLDEAKQLAHKIAAKSPLSVRATIELLNAAKEKSFAEAVRDEAEWFGRVFVSEDGR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
EGVQAFLEKRPPVFQGK
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEHFRLTKEEFVAVVTFSRPPANALSSAVLKELSNILDELEADSDVRVVLLHGEGRFFSA
CCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEC
GADIKEFTSVASAEEASALSRNGQLVVERIERFSKPVIAAIHGAALGGGLELAMSYRIRL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEEEEEEE
VTENAKLGLPELQLGIIPGFAGTQRLVRYVGFGKAAEMMWTGEPITGLEAVQWGLANKAV
EECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHCCCCC
PEERLLDEAKQLAHKIAAKSPLSVRATIELLNAAKEKSFAEAVRDEAEWFGRVFVSEDGR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
EGVQAFLEKRPPVFQGK
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]