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The map label for this gene is spo0A [H]

Identifier: 138895958

GI number: 138895958

Start: 2438986

End: 2439765

Strand: Reverse

Name: spo0A [H]

Synonym: GTNG_2317

Alternate gene names: 138895958

Gene position: 2439765-2438986 (Counterclockwise)

Preceding gene: 138895959

Following gene: 138895955

Centisome position: 68.72

GC content: 46.92

Gene sequence:

>780_bases
TTGAGCATTAAAGTGTGCATTGCCGACGATAACCGTGAATTGGTGACGTTGCTTGATGAATATATTTCGAATCAGTCGGA
CATGGAAGTGATTGGTACAGCATATAACGGCCAAGACTGCTTGCATATGCTTGAAGAGAAGCAGCCGGACATTTTGATTT
TGGATATCATTATGCCACATTTAGACGGGTTGGCCGTGCTTGAGAAAGTTCGTACTAGCTTTGAGCACCAGCCGAACGTT
ATCATGTTGACGGCGTTTGGTCAGGAAGATGTGACGAAAAAAGCGGTCGAACTCGGCGCTTCCTATTTTATTTTAAAACC
GTTTGATATGGAAAATTTAGTTCATCATATCCGACAAATTTACGGAAAAACCGCACCGGCTGCTAAAAAAGTAGCCCCGG
CGTATCAAGCGCGCGACAATAAGCCGAAAAACTTGGATGCGAGCATTACGAGCATCATCCATGAAATCGGCGTCCCTGCC
CATATTAAAGGGTATTTGTATTTGCGCGAGGCGATCGCCATGGTGTATCACGACATCGAATTGCTCGGTTCGATCACCAA
AGTGCTGTATCCTGATATCGCTAAAAAATACAACACGACCGCCAGCCGTGTCGAGCGAGCCATCCGTCATGCGATTGAAG
TTGCATGGAGCCGAGGCAACCTCGAATCGATTTCCTCGCTGTTCGGCTACACTGTCAGCGTCTCGAAAGCGAAGCCGACG
AATTCCGAATTTATCGCGATGGTAGCCGACAAGCTAAGATTGGAACATAAGGCGTCGTAA

Upstream 100 bases:

>100_bases
TTGCTAATTGATGAAAAATAAAAGGATTTTTTGCCACTGTTGTCGAATGTACGTTAACGGAATATTACATAGCAAGAAAA
AATGGGGGAGGACGATTGGT

Downstream 100 bases:

>100_bases
CGATGAGAGAAAATCATGGCATGACGTCAGGTGTCCCATGGATGGGACACCTTTTCCTGTTGATGATGCACAATGCGGTT
CAAGGCCGGCGATTCGCCTA

Product: stage 0 sporulation protein A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSIKVCIADDNRELVTLLDEYISNQSDMEVIGTAYNGQDCLHMLEEKQPDILILDIIMPHLDGLAVLEKVRTSFEHQPNV
IMLTAFGQEDVTKKAVELGASYFILKPFDMENLVHHIRQIYGKTAPAAKKVAPAYQARDNKPKNLDASITSIIHEIGVPA
HIKGYLYLREAIAMVYHDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNLESISSLFGYTVSVSKAKPT
NSEFIAMVADKLRLEHKAS

Sequences:

>Translated_259_residues
MSIKVCIADDNRELVTLLDEYISNQSDMEVIGTAYNGQDCLHMLEEKQPDILILDIIMPHLDGLAVLEKVRTSFEHQPNV
IMLTAFGQEDVTKKAVELGASYFILKPFDMENLVHHIRQIYGKTAPAAKKVAPAYQARDNKPKNLDASITSIIHEIGVPA
HIKGYLYLREAIAMVYHDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNLESISSLFGYTVSVSKAKPT
NSEFIAMVADKLRLEHKAS
>Mature_258_residues
SIKVCIADDNRELVTLLDEYISNQSDMEVIGTAYNGQDCLHMLEEKQPDILILDIIMPHLDGLAVLEKVRTSFEHQPNVI
MLTAFGQEDVTKKAVELGASYFILKPFDMENLVHHIRQIYGKTAPAAKKVAPAYQARDNKPKNLDASITSIIHEIGVPAH
IKGYLYLREAIAMVYHDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNLESISSLFGYTVSVSKAKPTN
SEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=107, Percent_Identity=38.3177570093458, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=80, Percent_Identity=36.25, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788724, Length=144, Percent_Identity=29.8611111111111, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI87082052, Length=120, Percent_Identity=34.1666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=28.6885245901639, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 28994; Mature: 28863

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKVCIADDNRELVTLLDEYISNQSDMEVIGTAYNGQDCLHMLEEKQPDILILDIIMPH
CEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEHHHC
LDGLAVLEKVRTSFEHQPNVIMLTAFGQEDVTKKAVELGASYFILKPFDMENLVHHIRQI
CCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
YGKTAPAAKKVAPAYQARDNKPKNLDASITSIIHEIGVPAHIKGYLYLREAIAMVYHDIE
HCCCCCHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNLESISSLFGYTVSVSKAKPT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCC
NSEFIAMVADKLRLEHKAS
CHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIKVCIADDNRELVTLLDEYISNQSDMEVIGTAYNGQDCLHMLEEKQPDILILDIIMPH
EEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEHHHC
LDGLAVLEKVRTSFEHQPNVIMLTAFGQEDVTKKAVELGASYFILKPFDMENLVHHIRQI
CCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
YGKTAPAAKKVAPAYQARDNKPKNLDASITSIIHEIGVPAHIKGYLYLREAIAMVYHDIE
HCCCCCHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNLESISSLFGYTVSVSKAKPT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCC
NSEFIAMVADKLRLEHKAS
CHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10089466; 7885226; 10556024; 10731426 [H]