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The map label for this gene is rluB [H]

Identifier: 138895852

GI number: 138895852

Start: 2336260

End: 2336991

Strand: Reverse

Name: rluB [H]

Synonym: GTNG_2211

Alternate gene names: 138895852

Gene position: 2336991-2336260 (Counterclockwise)

Preceding gene: 138895853

Following gene: 138895851

Centisome position: 65.82

GC content: 53.83

Gene sequence:

>732_bases
ATGGAACGGTTACAAAAAGTGATTGCCCGCGCCGGTATTGCGTCAAGGCGCAAGGCGGAAGAAATGATTTTGCAAGGGCG
GGTGAAGGTCAATGGCCGCGTCGTCACCGAACTTGGTGTCAAAGTCGGACCGCGTGACGATGTGGAAGTTGACGGCATTC
CGGTCGAGCGCGAGGAGCCGGTCTATTATTTGCTTTACAAGCCGCGCGGCGTCATCTCGAGCGTTAAAGATGAGAAGGGG
CGCAAAGTGGTGACCGACTTTTTTAAAGACATCCACCAGCGCATTTACCCGATCGGACGGCTTGACTACGATACGTCCGG
TCTGCTCTTGTTGACGAATGACGGCGATTTTGCTAATTTGCTGATGCATCCGCGCTATGAGATTGAGAAGGTGTACGTCG
CTAAAGTGAAAGGCATCCCGACACGCGAGCAGCTGAAACAGCCTGAGAAAGGGGTGCTGTTGGAAGACGGCATAACGGCG
CCCGCAAAAGTGAAGCTGCTCTCAGCTGATCGGAAAAAGCGGGCAGCGATTGTCGAGATTAGCATTCATGAAGGACGGAA
TCGGCAAGTGCGCCGCATGTTTGAGGCGATCGGCTGTGAAGTGCTGAAGCTGAAGCGGGAACGGTATGCGTTTCTTGATT
TAAAAGGGCTTCGCCCCGGCGAATATCGTGAACTGTCACCGCATGAAGTGAAGCTGTTGCGGACGATGGCCCAATCCGGG
GGAAAGAAATAG

Upstream 100 bases:

>100_bases
TTGGAAGCGAGGATTCTCAGTTTGGGATGATAAAAATAAAAGCGACATGCTTGTATCTGTCGAACGGAAAGGGTAAGATG
ATGAAAGAGGTGAGAAGGAA

Downstream 100 bases:

>100_bases
TGACAAACTTTGTCACACAACGTTCATATCCTCATAGATAGGGTAAAAATAGACTTTGCTATAATGGAAAATGGAAGATT
ATGAACTGTCACGTAAACCC

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MERLQKVIARAGIASRRKAEEMILQGRVKVNGRVVTELGVKVGPRDDVEVDGIPVEREEPVYYLLYKPRGVISSVKDEKG
RKVVTDFFKDIHQRIYPIGRLDYDTSGLLLLTNDGDFANLLMHPRYEIEKVYVAKVKGIPTREQLKQPEKGVLLEDGITA
PAKVKLLSADRKKRAAIVEISIHEGRNRQVRRMFEAIGCEVLKLKRERYAFLDLKGLRPGEYRELSPHEVKLLRTMAQSG
GKK

Sequences:

>Translated_243_residues
MERLQKVIARAGIASRRKAEEMILQGRVKVNGRVVTELGVKVGPRDDVEVDGIPVEREEPVYYLLYKPRGVISSVKDEKG
RKVVTDFFKDIHQRIYPIGRLDYDTSGLLLLTNDGDFANLLMHPRYEIEKVYVAKVKGIPTREQLKQPEKGVLLEDGITA
PAKVKLLSADRKKRAAIVEISIHEGRNRQVRRMFEAIGCEVLKLKRERYAFLDLKGLRPGEYRELSPHEVKLLRTMAQSG
GKK
>Mature_243_residues
MERLQKVIARAGIASRRKAEEMILQGRVKVNGRVVTELGVKVGPRDDVEVDGIPVEREEPVYYLLYKPRGVISSVKDEKG
RKVVTDFFKDIHQRIYPIGRLDYDTSGLLLLTNDGDFANLLMHPRYEIEKVYVAKVKGIPTREQLKQPEKGVLLEDGITA
PAKVKLLSADRKKRAAIVEISIHEGRNRQVRRMFEAIGCEVLKLKRERYAFLDLKGLRPGEYRELSPHEVKLLRTMAQSG
GKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=39.8373983739837, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=35.3448275862069, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=36.0995850622407, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=36.9318181818182, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27716; Mature: 27716

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIARAGIASRRKAEEMILQGRVKVNGRVVTELGVKVGPRDDVEVDGIPVEREEP
CHHHHHHHHHHCCHHHHHHHHHHHHCEEEECCEEEEEECCCCCCCCCCEECCCCCCCCCC
VYYLLYKPRGVISSVKDEKGRKVVTDFFKDIHQRIYPIGRLDYDTSGLLLLTNDGDFANL
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHH
LMHPRYEIEKVYVAKVKGIPTREQLKQPEKGVLLEDGITAPAKVKLLSADRKKRAAIVEI
EECCCHHHHEEEEEEECCCCCHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCEEEEEE
SIHEGRNRQVRRMFEAIGCEVLKLKRERYAFLDLKGLRPGEYRELSPHEVKLLRTMAQSG
EECCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GKK
CCC
>Mature Secondary Structure
MERLQKVIARAGIASRRKAEEMILQGRVKVNGRVVTELGVKVGPRDDVEVDGIPVEREEP
CHHHHHHHHHHCCHHHHHHHHHHHHCEEEECCEEEEEECCCCCCCCCCEECCCCCCCCCC
VYYLLYKPRGVISSVKDEKGRKVVTDFFKDIHQRIYPIGRLDYDTSGLLLLTNDGDFANL
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHH
LMHPRYEIEKVYVAKVKGIPTREQLKQPEKGVLLEDGITAPAKVKLLSADRKKRAAIVEI
EECCCHHHHEEEEEEECCCCCHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCEEEEEE
SIHEGRNRQVRRMFEAIGCEVLKLKRERYAFLDLKGLRPGEYRELSPHEVKLLRTMAQSG
EECCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]