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The map label for this gene is sleB [H]

Identifier: 138895808

GI number: 138895808

Start: 2295075

End: 2295866

Strand: Reverse

Name: sleB [H]

Synonym: GTNG_2167

Alternate gene names: 138895808

Gene position: 2295866-2295075 (Counterclockwise)

Preceding gene: 138895809

Following gene: 138895807

Centisome position: 64.67

GC content: 54.04

Gene sequence:

>792_bases
ATGGTAAGGAAACTCATTGTGTTCACCTTAATAGGCGTCTTAGTATGCCTGCATCCAGCAGGCGATGCTGCCGCCTTTTC
CCCGCAAGTCATCCAGCGTGGAGCGGTCGGCGATGATGTCATTGAATTGCAGGCGCGACTGCAATATCTCGGTTTTTACA
AGGGAAAAATTGACGGCGTGTTCGGTTGGGGCACGTATTGGGCGCTGCGCAACTTCCAACAAAAATACGGCCTGCCAGTC
GACGGGTTGGCCGGTGCCGAGACGAAACGGAAGTTGGTGAAGGCCTCCAAATATTATGAGCAGTTTGTCAAAGAGCAAAT
TCGCCAAGGAAACGAATTTACCCATTACGGGGGCATCCCGCTCAGCCAGCAAGTGAAAAAGCGCGGCAGCGGGGGAAGAA
CGGCAAAAACGACGGCGTCGAACGTGCCGAAAGGGTTTTCGCAAAACGATATTCAGTTGATGGCGAACGCGGTTTATGGT
GAAGCGCGCGGCGAGCCGTACATCGGCCAGGTAGCCGTCGCCGCCGTCATTTTAAACCGGCTCCAACACCCGTCGTTCCC
GGATACGGTTGCTGGAGTCATTTTTCAACCGGGCGCGTTTACTGCGGTCGCTGACGGACAAATTTGGCTGACGCCAAACG
AAACAGCAAGAAAGGCAGTGCTTGATGCCATTAACGGTTGGGACCCGAGCGGTGGCGCCATTTACTATTTCAACCCGGCA
ACCGCGACGAGCGCTTGGATTTGGAGTCGGCCGCAAATTAAACGGATCGGGCAGCATATTTTCTGCAAATAA

Upstream 100 bases:

>100_bases
ACGGCTTTCCGGACGGCCCCTTCTTCTTTTTTCTGTATAAAGAGGAAAGACCGGAGGAAAATAAGGATAAAAACGGCGAT
GGTCGACGGGAGGTTTGCAT

Downstream 100 bases:

>100_bases
AAGGAGGGAGACGGAATGGTGCGAAACCTATTGCTTGCGGTGCTCGTCGTTGCCGTCGTCGGCACCGGTTATTGGGGCTA
CCGCGAGCACTCGGAGAAAA

Product: sporulation specific N-acetylmuramoyl-L-alanineamidase

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MVRKLIVFTLIGVLVCLHPAGDAAAFSPQVIQRGAVGDDVIELQARLQYLGFYKGKIDGVFGWGTYWALRNFQQKYGLPV
DGLAGAETKRKLVKASKYYEQFVKEQIRQGNEFTHYGGIPLSQQVKKRGSGGRTAKTTASNVPKGFSQNDIQLMANAVYG
EARGEPYIGQVAVAAVILNRLQHPSFPDTVAGVIFQPGAFTAVADGQIWLTPNETARKAVLDAINGWDPSGGAIYYFNPA
TATSAWIWSRPQIKRIGQHIFCK

Sequences:

>Translated_263_residues
MVRKLIVFTLIGVLVCLHPAGDAAAFSPQVIQRGAVGDDVIELQARLQYLGFYKGKIDGVFGWGTYWALRNFQQKYGLPV
DGLAGAETKRKLVKASKYYEQFVKEQIRQGNEFTHYGGIPLSQQVKKRGSGGRTAKTTASNVPKGFSQNDIQLMANAVYG
EARGEPYIGQVAVAAVILNRLQHPSFPDTVAGVIFQPGAFTAVADGQIWLTPNETARKAVLDAINGWDPSGGAIYYFNPA
TATSAWIWSRPQIKRIGQHIFCK
>Mature_263_residues
MVRKLIVFTLIGVLVCLHPAGDAAAFSPQVIQRGAVGDDVIELQARLQYLGFYKGKIDGVFGWGTYWALRNFQQKYGLPV
DGLAGAETKRKLVKASKYYEQFVKEQIRQGNEFTHYGGIPLSQQVKKRGSGGRTAKTTASNVPKGFSQNDIQLMANAVYG
EARGEPYIGQVAVAAVILNRLQHPSFPDTVAGVIFQPGAFTAVADGQIWLTPNETARKAVLDAINGWDPSGGAIYYFNPA
TATSAWIWSRPQIKRIGQHIFCK

Specific function: Required for spore cortex hydrolysis during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 28793; Mature: 28793

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKLIVFTLIGVLVCLHPAGDAAAFSPQVIQRGAVGDDVIELQARLQYLGFYKGKIDGV
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCE
FGWGTYWALRNFQQKYGLPVDGLAGAETKRKLVKASKYYEQFVKEQIRQGNEFTHYGGIP
ECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LSQQVKKRGSGGRTAKTTASNVPKGFSQNDIQLMANAVYGEARGEPYIGQVAVAAVILNR
HHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LQHPSFPDTVAGVIFQPGAFTAVADGQIWLTPNETARKAVLDAINGWDPSGGAIYYFNPA
HCCCCCCHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCC
TATSAWIWSRPQIKRIGQHIFCK
CCCHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVRKLIVFTLIGVLVCLHPAGDAAAFSPQVIQRGAVGDDVIELQARLQYLGFYKGKIDGV
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCE
FGWGTYWALRNFQQKYGLPVDGLAGAETKRKLVKASKYYEQFVKEQIRQGNEFTHYGGIP
ECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LSQQVKKRGSGGRTAKTTASNVPKGFSQNDIQLMANAVYGEARGEPYIGQVAVAAVILNR
HHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LQHPSFPDTVAGVIFQPGAFTAVADGQIWLTPNETARKAVLDAINGWDPSGGAIYYFNPA
HCCCCCCHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCC
TATSAWIWSRPQIKRIGQHIFCK
CCCHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]