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The map label for this gene is paaJ [H]

Identifier: 138895567

GI number: 138895567

Start: 2043748

End: 2044959

Strand: Reverse

Name: paaJ [H]

Synonym: GTNG_1920

Alternate gene names: 138895567

Gene position: 2044959-2043748 (Counterclockwise)

Preceding gene: 138895568

Following gene: 138895566

Centisome position: 57.6

GC content: 56.19

Gene sequence:

>1212_bases
ATGGGAAGAGAGGTTGTCATTGTTGATGCTGTGCGCACACCGATCGGCCGCTACAAAGGGGCATTAAAAGAGGTGCGGCC
GGACGATTTGGCCGCCGTCGTTATTCGCGCGCTGATCGAGCGGAATCCGAACTTGCCGGTGGAACAAATTGATGACGTCG
TCTTCGGTAATGCGAATCAAGCCGGTGAAGACAACCGGAACGTTGCCCGCATGGCCGCACTTTTAGCCGGATTGCCGAAG
GAAGTAGCGGGGGTAACGATCAATCGGTTGTGCGGTTCCGGGCTCGATGCTGTTAATTATGCAGCGCGGGCCATTTTCGC
CGATGAGGCGGATATTATGATCGCCGGCGGCGTGGAAAGCATGACGCGCGCTCCGTTTGTGATGGCGAAGCCGTCAAGTG
ATTTTCCACGCGGCAATATCGAAATGTATGATACAACGATCGGCTGGCGCTTTATCAATCCGAAAATGCAGGAAATGTAC
GGCACCGACAGCATGCCACAAACGGCGGAAAACGTAGCGAAGCGGTTTGGCATTACGCGCGAGGCGCAGGACGAGTTTGC
CTATGAGAGCCAAATGAAAGCGAAACGGGCGATGGAAACGAACCGGTTTGCGGATGAATTGGTGCCAGTTGTGTACTATG
ATCGGAAAGGCAACCGGATTGTTGTCGACAAGGATGAGCATCCGCGCCCGGATACGACGCTTGAGAAGCTGGCAAAACTG
CCGCCGCTGTTTGAAAACGGCACGGTGACAGCCGGCAATGCTTCTGGAGTGAACGACGGGGCGGCGGCGCTGTTGTTGAT
GAGTGCCGATAAAGCGAAAGCGCTCGGCTTGAAGCCGTTAGCCAAATATGTGACGTCAGCCGTCGCCGGCGTTGAACCAG
CCGTAATGGGAATCGGTCCGATTTTTGCGACGCGCAAAGCGCTGCGCCGCGCCGGCCTGTCCGTTGATGACATCGGGCTT
GTCGAGCTGAACGAAGCGTTCGCTTCGCAAGCGCTCGAGTGCATTCGTCAGCTTGAGCTTGACCGAGAGAAAGTCAACGT
CAACGGCGGAGCGATTGCCCTTGGCCATCCGCTTGGGGCGAGTGGTGCCCGCATATTGACGACGTTGATTTATGAAATGC
AAAAACGAGGCGTCACCTACGGCTTGGCGACGATGTGCATCGGCGTCGGCCAAGGGATCGCAACGATTGTCAAAAATGCG
GACGAACAGTGA

Upstream 100 bases:

>100_bases
GTCTTGGCCGCAAAACTGGCCGTGGTGTCTATGATTATACGAATCGAGAAAACGACTGACAAGCCGATCGCTCGGCTACG
GTTGGCAAGGGGGAGAAAAG

Downstream 100 bases:

>100_bases
GAGCGTTCGGCTAGGGGTTGAGAGATACCTAGTCATGATGGAGGGATACTTGAGTGTGCCGCTGTTTCCTCAATACCGGC
CATTCGATCATTCCGTCCAA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MGREVVIVDAVRTPIGRYKGALKEVRPDDLAAVVIRALIERNPNLPVEQIDDVVFGNANQAGEDNRNVARMAALLAGLPK
EVAGVTINRLCGSGLDAVNYAARAIFADEADIMIAGGVESMTRAPFVMAKPSSDFPRGNIEMYDTTIGWRFINPKMQEMY
GTDSMPQTAENVAKRFGITREAQDEFAYESQMKAKRAMETNRFADELVPVVYYDRKGNRIVVDKDEHPRPDTTLEKLAKL
PPLFENGTVTAGNASGVNDGAAALLLMSADKAKALGLKPLAKYVTSAVAGVEPAVMGIGPIFATRKALRRAGLSVDDIGL
VELNEAFASQALECIRQLELDREKVNVNGGAIALGHPLGASGARILTTLIYEMQKRGVTYGLATMCIGVGQGIATIVKNA
DEQ

Sequences:

>Translated_403_residues
MGREVVIVDAVRTPIGRYKGALKEVRPDDLAAVVIRALIERNPNLPVEQIDDVVFGNANQAGEDNRNVARMAALLAGLPK
EVAGVTINRLCGSGLDAVNYAARAIFADEADIMIAGGVESMTRAPFVMAKPSSDFPRGNIEMYDTTIGWRFINPKMQEMY
GTDSMPQTAENVAKRFGITREAQDEFAYESQMKAKRAMETNRFADELVPVVYYDRKGNRIVVDKDEHPRPDTTLEKLAKL
PPLFENGTVTAGNASGVNDGAAALLLMSADKAKALGLKPLAKYVTSAVAGVEPAVMGIGPIFATRKALRRAGLSVDDIGL
VELNEAFASQALECIRQLELDREKVNVNGGAIALGHPLGASGARILTTLIYEMQKRGVTYGLATMCIGVGQGIATIVKNA
DEQ
>Mature_402_residues
GREVVIVDAVRTPIGRYKGALKEVRPDDLAAVVIRALIERNPNLPVEQIDDVVFGNANQAGEDNRNVARMAALLAGLPKE
VAGVTINRLCGSGLDAVNYAARAIFADEADIMIAGGVESMTRAPFVMAKPSSDFPRGNIEMYDTTIGWRFINPKMQEMYG
TDSMPQTAENVAKRFGITREAQDEFAYESQMKAKRAMETNRFADELVPVVYYDRKGNRIVVDKDEHPRPDTTLEKLAKLP
PLFENGTVTAGNASGVNDGAAALLLMSADKAKALGLKPLAKYVTSAVAGVEPAVMGIGPIFATRKALRRAGLSVDDIGLV
ELNEAFASQALECIRQLELDREKVNVNGGAIALGHPLGASGARILTTLIYEMQKRGVTYGLATMCIGVGQGIATIVKNAD
EQ

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=43.609022556391, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=41.8136020151133, Blast_Score=298, Evalue=7e-81,
Organism=Homo sapiens, GI4501853, Length=405, Percent_Identity=44.9382716049383, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=40.8521303258145, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=33.7236533957845, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI194353979, Length=255, Percent_Identity=36.078431372549, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=60.4010025062657, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI87082165, Length=397, Percent_Identity=47.3551637279597, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=50.5025125628141, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=46.8827930174564, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=32.3671497584541, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=42.9292929292929, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=389, Percent_Identity=36.7609254498715, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=36.25, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=30.5882352941176, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=28.8265306122449, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=40.8415841584158, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=38.0597014925373, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24655093, Length=396, Percent_Identity=44.9494949494949, Blast_Score=317, Evalue=8e-87,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=40.4466501240695, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=38.1313131313131, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=32.3255813953488, Blast_Score=178, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 43338; Mature: 43207

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGREVVIVDAVRTPIGRYKGALKEVRPDDLAAVVIRALIERNPNLPVEQIDDVVFGNANQ
CCCEEEEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
AGEDNRNVARMAALLAGLPKEVAGVTINRLCGSGLDAVNYAARAIFADEADIMIAGGVES
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHH
MTRAPFVMAKPSSDFPRGNIEMYDTTIGWRFINPKMQEMYGTDSMPQTAENVAKRFGITR
HHCCCEEEECCCCCCCCCCEEEEEEEECEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCH
EAQDEFAYESQMKAKRAMETNRFADELVPVVYYDRKGNRIVVDKDEHPRPDTTLEKLAKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHC
PPLFENGTVTAGNASGVNDGAAALLLMSADKAKALGLKPLAKYVTSAVAGVEPAVMGIGP
CCCCCCCEEECCCCCCCCCCHHEEEEEECCCHHHCCHHHHHHHHHHHHHCCCHHHHHCCH
IFATRKALRRAGLSVDDIGLVELNEAFASQALECIRQLELDREKVNVNGGAIALGHPLGA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCEEEECCCCCC
SGARILTTLIYEMQKRGVTYGLATMCIGVGQGIATIVKNADEQ
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
GREVVIVDAVRTPIGRYKGALKEVRPDDLAAVVIRALIERNPNLPVEQIDDVVFGNANQ
CCEEEEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
AGEDNRNVARMAALLAGLPKEVAGVTINRLCGSGLDAVNYAARAIFADEADIMIAGGVES
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHH
MTRAPFVMAKPSSDFPRGNIEMYDTTIGWRFINPKMQEMYGTDSMPQTAENVAKRFGITR
HHCCCEEEECCCCCCCCCCEEEEEEEECEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCH
EAQDEFAYESQMKAKRAMETNRFADELVPVVYYDRKGNRIVVDKDEHPRPDTTLEKLAKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHC
PPLFENGTVTAGNASGVNDGAAALLLMSADKAKALGLKPLAKYVTSAVAGVEPAVMGIGP
CCCCCCCEEECCCCCCCCCCHHEEEEEECCCHHHCCHHHHHHHHHHHHHCCCHHHHHCCH
IFATRKALRRAGLSVDDIGLVELNEAFASQALECIRQLELDREKVNVNGGAIALGHPLGA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCEEEECCCCCC
SGARILTTLIYEMQKRGVTYGLATMCIGVGQGIATIVKNADEQ
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]