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The map label for this gene is ybbF [H]

Identifier: 138895527

GI number: 138895527

Start: 2003039

End: 2004403

Strand: Reverse

Name: ybbF [H]

Synonym: GTNG_1877

Alternate gene names: 138895527

Gene position: 2004403-2003039 (Counterclockwise)

Preceding gene: 138895528

Following gene: 138895526

Centisome position: 56.46

GC content: 50.99

Gene sequence:

>1365_bases
ATGACTCGAGAACAACAAATAGCAGCATCGATACTTCAGTATGTTGGGGGGAAAGAAAACATCACCCGCGTTGCCCATTG
CATGACGCGGGTGCGTGTATCGTTGCGCGATCCGGGCAAAGCGAACATTGACTCCTTAAAACAGATCGAAGGGGTCATGG
GAGTCATTGAGGATGAGACGTTGCAAATCGTCGTGGGCCCGGGCGTTGTCAATAAAGTAGCGGCTGCGTTATGCGAGCTT
ACCGGTTTAGAACTTGGAGAAGTGATTGAAGATGGAATCGAGGACATCGCGGAGCGGACAAAAGCGGAAGTCAAAGCGCG
TCAGCAAACGCCGCTAAAGCGATTGCTTCGCAAAATCGGCAGCATCTTCATCCCTCTTATTCCTGGGCTGGTTGCATCGG
GGATTATTAACGGAATTGCCAACTTTGCGAAAAACGCCGGCGTTGATCCGCAGACGACGTGGCTGCAACTGTTGCTTTTG
ATAGGCGGCGGAATTTTTGCTTCGCTTGGCGTGTTAGTGGGATATAATACAGCGAAAGAGTTTGGCGGCACTCCTGTATT
AGGGGCGATTGCTGGTATTCTCGTATTCAACCCAGCGATGGGCGATATAAAATTATTTGGGGAAGCGCTTACACCTGGAA
GGGGAGGACTGTTTGCGGTCTTGCTTGCCGCCTGGCTCATGGCGGTAGTCGAAAAACGGGTGCGTTCGTTTGTGCCAAAT
GCGGTCGATATTATCGTCACACCGCTCATTACCGTATTAATCGTCTGTCTATTTACCTTGATCGCTGTACAGCCGCTTGC
CGGTTGGTTGTCGTTAGCGATTACCAGCGGGCTGAAAGCAGTGCTCGATATCGGTGGAGTAGTCGCTGGGGCTGTGTTGG
CCGGAACGTTTTTGCCGCTTGTGATGGTCGGACTGCATCACGGATTAACCCCTATTCACATGGAATTGATTCAAAAATTG
GGCTCGACACCGCTACTGCCGGTATTGGCGATGGCTGGAGCCGGTCAAGTTGGAGCGGCGATTGCCGTGTACGTCAAAAC
AAAAAATAAACGGCTTCGTAACATTATCAAAGGGGCGTTGCCGGTCGGATTTTTAGGTATTGGGGAGCCGCTATTGTACG
GGGTAACATTACCGCTTGGGCGGCCGTTTGTTACCGCGTGCCTTGGGGCGGCGGTCGGTGGTGCATTTCAAGCAGTGATG
AAAACGGCGGCGCTTGGCATCGGCGTATCCGGGCTGTCACTGATTCCGCTCATTGCTGATACAAAGTATGTGATTTATTT
CATCGGACTCGTCATTTCATACACGTTTGGCTTCCTATTTACGTATTGGTTCGGGTTTAAAGAAGAAATGGCGGCACATA
TTTAA

Upstream 100 bases:

>100_bases
TGCGCCAATGCGGTTATGAAGAAGCAAAAGAACGACTGCAGCAAGCCGAAGGGTTTGTACGTAAAGCGCTTGAATAACGA
ATAAGGGGGAGAGAAATATC

Downstream 100 bases:

>100_bases
TGGGGGATGGCGATGCTTTCCTTTTCTTTCTACCTTTTTCAGCCGCTCGAGCAAATCGAAGAAACATTTCAGCTTGCAGC
GCGGCTTGGGGCGAAACAGT

Product: PTS system sucrose-specific IIBC

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTREQQIAASILQYVGGKENITRVAHCMTRVRVSLRDPGKANIDSLKQIEGVMGVIEDETLQIVVGPGVVNKVAAALCEL
TGLELGEVIEDGIEDIAERTKAEVKARQQTPLKRLLRKIGSIFIPLIPGLVASGIINGIANFAKNAGVDPQTTWLQLLLL
IGGGIFASLGVLVGYNTAKEFGGTPVLGAIAGILVFNPAMGDIKLFGEALTPGRGGLFAVLLAAWLMAVVEKRVRSFVPN
AVDIIVTPLITVLIVCLFTLIAVQPLAGWLSLAITSGLKAVLDIGGVVAGAVLAGTFLPLVMVGLHHGLTPIHMELIQKL
GSTPLLPVLAMAGAGQVGAAIAVYVKTKNKRLRNIIKGALPVGFLGIGEPLLYGVTLPLGRPFVTACLGAAVGGAFQAVM
KTAALGIGVSGLSLIPLIADTKYVIYFIGLVISYTFGFLFTYWFGFKEEMAAHI

Sequences:

>Translated_454_residues
MTREQQIAASILQYVGGKENITRVAHCMTRVRVSLRDPGKANIDSLKQIEGVMGVIEDETLQIVVGPGVVNKVAAALCEL
TGLELGEVIEDGIEDIAERTKAEVKARQQTPLKRLLRKIGSIFIPLIPGLVASGIINGIANFAKNAGVDPQTTWLQLLLL
IGGGIFASLGVLVGYNTAKEFGGTPVLGAIAGILVFNPAMGDIKLFGEALTPGRGGLFAVLLAAWLMAVVEKRVRSFVPN
AVDIIVTPLITVLIVCLFTLIAVQPLAGWLSLAITSGLKAVLDIGGVVAGAVLAGTFLPLVMVGLHHGLTPIHMELIQKL
GSTPLLPVLAMAGAGQVGAAIAVYVKTKNKRLRNIIKGALPVGFLGIGEPLLYGVTLPLGRPFVTACLGAAVGGAFQAVM
KTAALGIGVSGLSLIPLIADTKYVIYFIGLVISYTFGFLFTYWFGFKEEMAAHI
>Mature_453_residues
TREQQIAASILQYVGGKENITRVAHCMTRVRVSLRDPGKANIDSLKQIEGVMGVIEDETLQIVVGPGVVNKVAAALCELT
GLELGEVIEDGIEDIAERTKAEVKARQQTPLKRLLRKIGSIFIPLIPGLVASGIINGIANFAKNAGVDPQTTWLQLLLLI
GGGIFASLGVLVGYNTAKEFGGTPVLGAIAGILVFNPAMGDIKLFGEALTPGRGGLFAVLLAAWLMAVVEKRVRSFVPNA
VDIIVTPLITVLIVCLFTLIAVQPLAGWLSLAITSGLKAVLDIGGVVAGAVLAGTFLPLVMVGLHHGLTPIHMELIQKLG
STPLLPVLAMAGAGQVGAAIAVYVKTKNKRLRNIIKGALPVGFLGIGEPLLYGVTLPLGRPFVTACLGAAVGGAFQAVMK
TAALGIGVSGLSLIPLIADTKYVIYFIGLVISYTFGFLFTYWFGFKEEMAAHI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=478, Percent_Identity=38.4937238493724, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790159, Length=423, Percent_Identity=28.3687943262411, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI2367362, Length=441, Percent_Identity=25.8503401360544, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI48994906, Length=481, Percent_Identity=27.6507276507277, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47628; Mature: 47496

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREQQIAASILQYVGGKENITRVAHCMTRVRVSLRDPGKANIDSLKQIEGVMGVIEDET
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
LQIVVGPGVVNKVAAALCELTGLELGEVIEDGIEDIAERTKAEVKARQQTPLKRLLRKIG
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SIFIPLIPGLVASGIINGIANFAKNAGVDPQTTWLQLLLLIGGGIFASLGVLVGYNTAKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCHHHH
FGGTPVLGAIAGILVFNPAMGDIKLFGEALTPGRGGLFAVLLAAWLMAVVEKRVRSFVPN
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AVDIIVTPLITVLIVCLFTLIAVQPLAGWLSLAITSGLKAVLDIGGVVAGAVLAGTFLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVGLHHGLTPIHMELIQKLGSTPLLPVLAMAGAGQVGAAIAVYVKTKNKRLRNIIKGAL
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHC
PVGFLGIGEPLLYGVTLPLGRPFVTACLGAAVGGAFQAVMKTAALGIGVSGLSLIPLIAD
CCCHHCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
TKYVIYFIGLVISYTFGFLFTYWFGFKEEMAAHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TREQQIAASILQYVGGKENITRVAHCMTRVRVSLRDPGKANIDSLKQIEGVMGVIEDET
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]