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The map label for this gene is blt [H]

Identifier: 138895161

GI number: 138895161

Start: 1584125

End: 1585339

Strand: Direct

Name: blt [H]

Synonym: GTNG_1499

Alternate gene names: 138895161

Gene position: 1584125-1585339 (Clockwise)

Preceding gene: 138895160

Following gene: 138895163

Centisome position: 44.62

GC content: 48.31

Gene sequence:

>1215_bases
ATGCAGCTGTTGTTTAAGAAGCGCGGAGCCATGCTGATTTTGATGTTCAATTTATTGCTGATTTTCATTGGCATCGGTTT
GATTATCCCGATCATGCCGACGTTTATCGAGCAATTGGGCATCTCAGGAGGAACGGTTGGACTGCTTGTGGCCGTTTTCT
CTTTAACACAATTTCTTTTTTCACCGGTAGCCGGCAGAATGGCAGATGCGGTCGGCCGAAAAAAATTGATCGTCTCAGGA
ATGCTGCTGTTTGCCTTTTCTGAGTGGCAGTTTGGCTATGCCGATCATGTTTTCGAGATGTTTTTTTCGCGGGTCATCGG
CGGGATAAGCGCCGCCCTCACCATGCCAGCAGTGATGGCTTATGCTGCGGACATAACGACGAATGAAGAACGGGCGGCAG
GCATTGGGTATATTAACGCAGCGATTACGATGGGATTTATGATTGGCCCAGGTATTGGCGGATACATGGCAGAATGGGGC
ATCCGCGTTCCGTTCAATTGTGCGGCGGCCGTTGGAGTGATGGCCGCTGTCATTACGGCGCTGTTTCTCCCGGAATCGCA
TTCATCTGCTTCCACGGGCACGGTTCAAAAGGATCACGTGCCGTTTTTTGTGCAGCTTGCCCAAGTTCATCGGCAACCGT
ATTTTTTCAGTTTGTTGATCATTTTTGCGCTGTCGTTTGGTCTTGCCAATTTTGAAACGATTTTCAGCTTGTTTGTCGAT
CATCGATTTGGCTTCTCACCGAAAGAAATCGCTTTGATCATTACAATTGGCTCGATTGGCGGCCCGCTCGTTCAGTTGAC
GACATTCGGCTGGTTGTTAGGCCACTTTGGTGAACAGCGCGTCGTTTCAGCTTGTTTGTTACTATCCGCCTTATTCATCG
TGTTGACGTTGTTGGCTTCGACATGGACGATGCTTTTAGTGGCAACGGTGTTAGTATTTTTGGCAATTGATATTTTGCGT
CCGGCGGTCAGCACGCAGATGTCTAAAATCGATGAGACCCAACAAGGCTATATCGCCGGCTTGAACTCCGCGTTTACAAG
CCTTGGAAATATTATTGGTCCAGCTGTTGCGGGCGTTTTGTTTGATGTAGATATTCGTTTTCCTTATATTCTCGCAAGCG
TTGTGCTTTTTCTATGCTTTGTGCTCTCATTACGGCAGGAAAAAGCGGTGCTCCACAAGGCCAGCAGCGATGCGTTGCGC
ATGACGAGAAAATGA

Upstream 100 bases:

>100_bases
TAAGCCGCCAAGTTCGGATGTACATGAAAGAATTTGTGGAAGGGAAAGGGTTTCATTTGCGGTAGTCTTTCTTCAGGCGG
AAGCAAAAAGGAGGGGAAAC

Downstream 100 bases:

>100_bases
AGAGAGACGCCAATAAAACAAGGAAGGATGATCGTGTGGAAAGTTGGCAGGCCAAAATATTTGGTGCTCCATCAACGAGG
AAGTGGCAAAATCATCTGTC

Product: multidrug ABC transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQLLFKKRGAMLILMFNLLLIFIGIGLIIPIMPTFIEQLGISGGTVGLLVAVFSLTQFLFSPVAGRMADAVGRKKLIVSG
MLLFAFSEWQFGYADHVFEMFFSRVIGGISAALTMPAVMAYAADITTNEERAAGIGYINAAITMGFMIGPGIGGYMAEWG
IRVPFNCAAAVGVMAAVITALFLPESHSSASTGTVQKDHVPFFVQLAQVHRQPYFFSLLIIFALSFGLANFETIFSLFVD
HRFGFSPKEIALIITIGSIGGPLVQLTTFGWLLGHFGEQRVVSACLLLSALFIVLTLLASTWTMLLVATVLVFLAIDILR
PAVSTQMSKIDETQQGYIAGLNSAFTSLGNIIGPAVAGVLFDVDIRFPYILASVVLFLCFVLSLRQEKAVLHKASSDALR
MTRK

Sequences:

>Translated_404_residues
MQLLFKKRGAMLILMFNLLLIFIGIGLIIPIMPTFIEQLGISGGTVGLLVAVFSLTQFLFSPVAGRMADAVGRKKLIVSG
MLLFAFSEWQFGYADHVFEMFFSRVIGGISAALTMPAVMAYAADITTNEERAAGIGYINAAITMGFMIGPGIGGYMAEWG
IRVPFNCAAAVGVMAAVITALFLPESHSSASTGTVQKDHVPFFVQLAQVHRQPYFFSLLIIFALSFGLANFETIFSLFVD
HRFGFSPKEIALIITIGSIGGPLVQLTTFGWLLGHFGEQRVVSACLLLSALFIVLTLLASTWTMLLVATVLVFLAIDILR
PAVSTQMSKIDETQQGYIAGLNSAFTSLGNIIGPAVAGVLFDVDIRFPYILASVVLFLCFVLSLRQEKAVLHKASSDALR
MTRK
>Mature_404_residues
MQLLFKKRGAMLILMFNLLLIFIGIGLIIPIMPTFIEQLGISGGTVGLLVAVFSLTQFLFSPVAGRMADAVGRKKLIVSG
MLLFAFSEWQFGYADHVFEMFFSRVIGGISAALTMPAVMAYAADITTNEERAAGIGYINAAITMGFMIGPGIGGYMAEWG
IRVPFNCAAAVGVMAAVITALFLPESHSSASTGTVQKDHVPFFVQLAQVHRQPYFFSLLIIFALSFGLANFETIFSLFVD
HRFGFSPKEIALIITIGSIGGPLVQLTTFGWLLGHFGEQRVVSACLLLSALFIVLTLLASTWTMLLVATVLVFLAIDILR
PAVSTQMSKIDETQQGYIAGLNSAFTSLGNIIGPAVAGVLFDVDIRFPYILASVVLFLCFVLSLRQEKAVLHKASSDALR
MTRK

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI217416366, Length=414, Percent_Identity=23.6714975845411, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI39753965, Length=335, Percent_Identity=26.2686567164179, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1787291, Length=341, Percent_Identity=24.633431085044, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71996331, Length=343, Percent_Identity=25.9475218658892, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357569, Length=339, Percent_Identity=25.9587020648968, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221513297, Length=343, Percent_Identity=26.2390670553936, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161080926, Length=339, Percent_Identity=25.9587020648968, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI161080924, Length=339, Percent_Identity=25.9587020648968, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24656463, Length=339, Percent_Identity=25.9587020648968, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24656468, Length=339, Percent_Identity=25.9587020648968, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43722; Mature: 43722

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]