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The map label for this gene is nfrA1 [H]

Identifier: 138895160

GI number: 138895160

Start: 1583340

End: 1584089

Strand: Direct

Name: nfrA1 [H]

Synonym: GTNG_1498

Alternate gene names: 138895160

Gene position: 1583340-1584089 (Clockwise)

Preceding gene: 138895159

Following gene: 138895161

Centisome position: 44.6

GC content: 50.0

Gene sequence:

>750_bases
ATGAATAAAGTCATCGAAACCATTTTACAGCACCGTTCGATCCGCCGTTTTGAAGACCGGCCGCTGACTGATGAACAAAT
TCGTATGATCGTTGAATGCGCTCAATCGGCGTCGACTTCAAGTTATATCCAAGCATATTCGATCATTGGGGTGAAAGACC
CGGAGAAAAAACGAAAGCTCGCCGAACTGGCAGGGAATCAATCCTATGTCGAGCATAACGGCCATTTCTTTGTCTTTTGC
GCTGATTTTCACCGCCACGAACAGATCGGTGAAATGGAAGAAAAAGACGTACTCCCATCGCTAGAAAGCACGGAAAAATT
TATGGTGGCGCTCATCGATACGGCGCTTGCGGCGCAAAATGCGGCGATTGCTGCTGAGTCGATGGGGCTTGGCATTTGCT
ATATCGGGGGGCTTCGCAACAACCTGCCGGAAGTATGCGAACTGCTTAACATACCGAAACGGGTCATTCCGCTGTTCGGG
CTTGCCGTCGGTTATCCAGCTGAACAGCCGGGAAAAAAACCGCGGTTGCCGTTAGACCACGTGTATCATGAGGATGAATA
CGATCAAGACCGCGGCCGGTTCATCGAGCAGTTGCAGCGATATAATGAAACGGTGTCAGCTTACTATGAACAGCGGACAA
ATGGCCGCCGCTCTGATACATGGACGGGGCAAATGGCTGACATGCTAAGCCGCCAAGTTCGGATGTACATGAAAGAATTT
GTGGAAGGGAAAGGGTTTCATTTGCGGTAG

Upstream 100 bases:

>100_bases
AAGGTTTATCTATTAGAGAAATACCTTGTTGCATTGCCTATTTGATTGACATCACTACTCTTAAGAATGAACCGAATTTT
TGGATGAGGAGGACAAGGAC

Downstream 100 bases:

>100_bases
TCTTTCTTCAGGCGGAAGCAAAAAGGAGGGGAAACATGCAGCTGTTGTTTAAGAAGCGCGGAGCCATGCTGATTTTGATG
TTCAATTTATTGCTGATTTT

Product: FMN-containing NADPH-linked nitro/flavin reductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNKVIETILQHRSIRRFEDRPLTDEQIRMIVECAQSASTSSYIQAYSIIGVKDPEKKRKLAELAGNQSYVEHNGHFFVFC
ADFHRHEQIGEMEEKDVLPSLESTEKFMVALIDTALAAQNAAIAAESMGLGICYIGGLRNNLPEVCELLNIPKRVIPLFG
LAVGYPAEQPGKKPRLPLDHVYHEDEYDQDRGRFIEQLQRYNETVSAYYEQRTNGRRSDTWTGQMADMLSRQVRMYMKEF
VEGKGFHLR

Sequences:

>Translated_249_residues
MNKVIETILQHRSIRRFEDRPLTDEQIRMIVECAQSASTSSYIQAYSIIGVKDPEKKRKLAELAGNQSYVEHNGHFFVFC
ADFHRHEQIGEMEEKDVLPSLESTEKFMVALIDTALAAQNAAIAAESMGLGICYIGGLRNNLPEVCELLNIPKRVIPLFG
LAVGYPAEQPGKKPRLPLDHVYHEDEYDQDRGRFIEQLQRYNETVSAYYEQRTNGRRSDTWTGQMADMLSRQVRMYMKEF
VEGKGFHLR
>Mature_249_residues
MNKVIETILQHRSIRRFEDRPLTDEQIRMIVECAQSASTSSYIQAYSIIGVKDPEKKRKLAELAGNQSYVEHNGHFFVFC
ADFHRHEQIGEMEEKDVLPSLESTEKFMVALIDTALAAQNAAIAAESMGLGICYIGGLRNNLPEVCELLNIPKRVIPLFG
LAVGYPAEQPGKKPRLPLDHVYHEDEYDQDRGRFIEQLQRYNETVSAYYEQRTNGRRSDTWTGQMADMLSRQVRMYMKEF
VEGKGFHLR

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=250, Percent_Identity=40.4, Blast_Score=187, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 28635; Mature: 28635

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVIETILQHRSIRRFEDRPLTDEQIRMIVECAQSASTSSYIQAYSIIGVKDPEKKRKL
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHH
AELAGNQSYVEHNGHFFVFCADFHRHEQIGEMEEKDVLPSLESTEKFMVALIDTALAAQN
HHHHCCCCEEEECCCEEEEECCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AAIAAESMGLGICYIGGLRNNLPEVCELLNIPKRVIPLFGLAVGYPAEQPGKKPRLPLDH
HHHHHHHCCCEEEEECCHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VYHEDEYDQDRGRFIEQLQRYNETVSAYYEQRTNGRRSDTWTGQMADMLSRQVRMYMKEF
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VEGKGFHLR
HCCCCCCCC
>Mature Secondary Structure
MNKVIETILQHRSIRRFEDRPLTDEQIRMIVECAQSASTSSYIQAYSIIGVKDPEKKRKL
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHH
AELAGNQSYVEHNGHFFVFCADFHRHEQIGEMEEKDVLPSLESTEKFMVALIDTALAAQN
HHHHCCCCEEEECCCEEEEECCHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AAIAAESMGLGICYIGGLRNNLPEVCELLNIPKRVIPLFGLAVGYPAEQPGKKPRLPLDH
HHHHHHHCCCEEEEECCHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VYHEDEYDQDRGRFIEQLQRYNETVSAYYEQRTNGRRSDTWTGQMADMLSRQVRMYMKEF
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VEGKGFHLR
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]