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The map label for this gene is miaA

Identifier: 138894831

GI number: 138894831

Start: 1236639

End: 1237586

Strand: Direct

Name: miaA

Synonym: GTNG_1167

Alternate gene names: 138894831

Gene position: 1236639-1237586 (Clockwise)

Preceding gene: 138894830

Following gene: 138894832

Centisome position: 34.83

GC content: 54.64

Gene sequence:

>948_bases
ATGACAGAAAAAATTGTTGTCATTGTCGGACCGACAGAAGTCGGCAAAACGAAGCTCGGCATTGCATTGGCGAAGAAGCT
AGGCGGGGAGATCATCAGCGGCGACTCGATGCAAATTTATAAAGGGATGGACATTGGCACCGCGAAAGTCAAGCCCGATG
AAATGGAAGGAGTTCCACACCATTTGCTTGACATTAAAGAGCCGTGCGAACCGTTTTCCGTCGTCGAATTTCAGCGGCTC
GCCCGCGCGCTCATCACCGATATTTCCGCCCGCGGCCGACTGCCGATCATCGTCGGCGGCACTGGGCTGTACATTCAAGC
TGCCATTTACGATTACCGATTTTCCGACGCCCCTTCCGATGAAGCATACCGTCGGGCGCTTAGGCAGTTGGCGGCTGAAC
AAGGAGCCGAGGCGCTTCACGAGCAGCTTAAGGCGGTCGACCCGGAAAGCGCGGCGCGCATTCATCCGAACAATGTCCGC
CGTGTCATTCGTGCGCTCGAAGTGTACCATTGCACGGGGAAACCGTTCAGTGAATGGCAGCGCGGACAGCAACGGCAACT
GTTATACGAAACGGCCCTCATCGGGCTGACAGCGGAGCGGAGCGTGCTTTACCGACGCATTAATGAACGGGTTGATGAGA
TGATCGCTGAAGGGCTGATTGAAGAGGTAAGGTCGCTATACGATCGCGGCCTGCGTGATTGTCAGGCAGTGCAGGCGATC
GGCTACAAAGAGCTGTACGACTATTTTGATGGCCGCGTCTCCCTCGACGAAGCGATCGAGCAGCTGAAGCAAAATTCGCG
CCGCTACGCGAAGCGGCAGCTGACGTGGTTTCGCAACCAAATGCCGGTAGAATGGTTTGACATGACGGATCCCGAAAAGT
TTGCCGTCAAGGTGGAGGAAATTTTTCGCTACATAGCAGGAAAGCTTCGACTTGAAGCGAATATATAA

Upstream 100 bases:

>100_bases
GGGATGGCGAAAACGTAGGAAAGAAATTGTTTCATGTGTTGCCGTTTTTATGTATAATGGTGTTTGATACGAGTTCAGCA
ACAGAAAGCAGTGGCACACC

Downstream 100 bases:

>100_bases
CCATATCCTTAGAGAGAAAAGAGGAGGACGTTCCATGAAAAATACGATCAACATTCAAGACCAGTTTTTAAACCAGTTGC
GCAAAGAAGGTATCCAAGTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTEKIVVIVGPTEVGKTKLGIALAKKLGGEIISGDSMQIYKGMDIGTAKVKPDEMEGVPHHLLDIKEPCEPFSVVEFQRL
ARALITDISARGRLPIIVGGTGLYIQAAIYDYRFSDAPSDEAYRRALRQLAAEQGAEALHEQLKAVDPESAARIHPNNVR
RVIRALEVYHCTGKPFSEWQRGQQRQLLYETALIGLTAERSVLYRRINERVDEMIAEGLIEEVRSLYDRGLRDCQAVQAI
GYKELYDYFDGRVSLDEAIEQLKQNSRRYAKRQLTWFRNQMPVEWFDMTDPEKFAVKVEEIFRYIAGKLRLEANI

Sequences:

>Translated_315_residues
MTEKIVVIVGPTEVGKTKLGIALAKKLGGEIISGDSMQIYKGMDIGTAKVKPDEMEGVPHHLLDIKEPCEPFSVVEFQRL
ARALITDISARGRLPIIVGGTGLYIQAAIYDYRFSDAPSDEAYRRALRQLAAEQGAEALHEQLKAVDPESAARIHPNNVR
RVIRALEVYHCTGKPFSEWQRGQQRQLLYETALIGLTAERSVLYRRINERVDEMIAEGLIEEVRSLYDRGLRDCQAVQAI
GYKELYDYFDGRVSLDEAIEQLKQNSRRYAKRQLTWFRNQMPVEWFDMTDPEKFAVKVEEIFRYIAGKLRLEANI
>Mature_314_residues
TEKIVVIVGPTEVGKTKLGIALAKKLGGEIISGDSMQIYKGMDIGTAKVKPDEMEGVPHHLLDIKEPCEPFSVVEFQRLA
RALITDISARGRLPIIVGGTGLYIQAAIYDYRFSDAPSDEAYRRALRQLAAEQGAEALHEQLKAVDPESAARIHPNNVRR
VIRALEVYHCTGKPFSEWQRGQQRQLLYETALIGLTAERSVLYRRINERVDEMIAEGLIEEVRSLYDRGLRDCQAVQAIG
YKELYDYFDGRVSLDEAIEQLKQNSRRYAKRQLTWFRNQMPVEWFDMTDPEKFAVKVEEIFRYIAGKLRLEANI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=341, Percent_Identity=34.6041055718475, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1790613, Length=308, Percent_Identity=42.5324675324675, Blast_Score=237, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=32.3432343234323, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=34.6938775510204, Blast_Score=189, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24649830, Length=331, Percent_Identity=35.9516616314199, Blast_Score=187, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_GEOTN (A4IMI5)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125284.1
- ProteinModelPortal:   A4IMI5
- SMR:   A4IMI5
- STRING:   A4IMI5
- GeneID:   4966107
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1167
- NMPDR:   fig|420246.5.peg.1135
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A4IMI5
- ProtClustDB:   PRK00091
- BioCyc:   GTHE420246:GTNG_1167-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35935; Mature: 35804

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKIVVIVGPTEVGKTKLGIALAKKLGGEIISGDSMQIYKGMDIGTAKVKPDEMEGVPH
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCHHHCCCCH
HLLDIKEPCEPFSVVEFQRLARALITDISARGRLPIIVGGTGLYIQAAIYDYRFSDAPSD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEEEECCCCCCH
EAYRRALRQLAAEQGAEALHEQLKAVDPESAARIHPNNVRRVIRALEVYHCTGKPFSEWQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RGQQRQLLYETALIGLTAERSVLYRRINERVDEMIAEGLIEEVRSLYDRGLRDCQAVQAI
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYKELYDYFDGRVSLDEAIEQLKQNSRRYAKRQLTWFRNQMPVEWFDMTDPEKFAVKVEE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
IFRYIAGKLRLEANI
HHHHHHHHHEEECCC
>Mature Secondary Structure 
TEKIVVIVGPTEVGKTKLGIALAKKLGGEIISGDSMQIYKGMDIGTAKVKPDEMEGVPH
CCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCHHHCCCCH
HLLDIKEPCEPFSVVEFQRLARALITDISARGRLPIIVGGTGLYIQAAIYDYRFSDAPSD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEEEECCCCCCH
EAYRRALRQLAAEQGAEALHEQLKAVDPESAARIHPNNVRRVIRALEVYHCTGKPFSEWQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RGQQRQLLYETALIGLTAERSVLYRRINERVDEMIAEGLIEEVRSLYDRGLRDCQAVQAI
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYKELYDYFDGRVSLDEAIEQLKQNSRRYAKRQLTWFRNQMPVEWFDMTDPEKFAVKVEE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
IFRYIAGKLRLEANI
HHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA