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The map label for this gene is miaB [H]

Identifier: 138894823

GI number: 138894823

Start: 1222144

End: 1223886

Strand: Direct

Name: miaB [H]

Synonym: GTNG_1157

Alternate gene names: 138894823

Gene position: 1222144-1223886 (Clockwise)

Preceding gene: 138894822

Following gene: 138894824

Centisome position: 34.42

GC content: 47.62

Gene sequence:

>1743_bases
ATGTTCCGGACATCCGGAACAAAAACCATTGTGTTTCATTGTCACCCGCGATATACTATGATAGTGTGTGTGATGGAAGA
ATTTTGCCGGAAAAAGGTTGACGGCAACGAAAACAACAGCTACCTAACTGTATGTAATCGAAAGTTGGACCAAGAAAGAA
AGGAGATTGTTATGAACGAAAAGCAACGTTTAGAACAAACAGGTCAAATTCAAACAGCTAGCCATCCAGCGGACAGAAAA
TCCGACTTGGAGCGGCTCAAAGCAAAAACGACGAAAGACTATGAGAAGTATTTCACGAGCGTCTTTCTTCCGCCGAACTT
AAAAGAGGCGAAAAAACGCGGCAAGGAAGAAGTACAATATGTGAAAGACTTTACGATTCCGGATGAATTCCGCGGTATGG
GGCGTGGGCGGAAGTTTTACATCCGCACGTACGGCTGTCAAATGAACGAGCATGATACGGAAGTGATGGCCGGCATTTTC
ATGGCGCTCGGCTATGAGCCGACCGACCGTCCGGAAGATGCAAATGTCATTTTGCTTAACACGTGTGCGATCCGTGAAAA
CGCGGAAAACAAAGTGTTTGGCGAACTCGGCTACTTAAAGCCGTTGAAAACGACGAACCCTGATTTACTACTTGGCGTGT
GCGGCTGTATGTCGCAAGAAGAAGCGGTCGTCAATAAAATTTTAAAACAATACCAGTATGTTGACTTGATTTTCGGCACG
CACAACATCCATCGCCTCCCGTACATTTTGCATGAGGCGTACATGTCGAAAGAGATGGTTGTCGAAGTATGGTCGAAAGA
GGGCGATGTCGTCGAAAACTTGCCGAAAGTGCGCAAAGGCAACATTAAAGCATGGGTCAACATTATGTATGGCTGCGATA
AGTTTTGTACGTACTGCATCGTTCCGTACACGCGCGGCAAAGAGCGGAGCCGTCGTCCGGAAGACATCATTCAAGAAGTG
CGCCAGCTCGCCGCCCAAGGCTATAAGGAAATTACGCTTCTTGGGCAAAACGTCAACGCCTACGGGAAAGATTTCAACGA
TATTCAATACGGTCTTGGCGATTTGATGGATGAGCTGCGCAAAATTGATATCGCGCGCATCCGCTTTACGACAAGCCATC
CGCGTGACTTTGACGACCGACTCATCGAAGTGCTTGCCAAACGAGGCAATTTAGTCGAACATATTCATTTGCCGGTGCAA
TCGGGCAGCACGGAAATTTTGAAAATGATGGGTCGGAAATATACACGTGAAGAGTATTTGGAACTCGTCCGCAAAATTAA
AGCGGCCATTCCGGATGTCGCTTTGACGACCGACATTATCGTCGGCTTCCCGAACGAAACGGATGAGCAGTTTGAAGAAA
CGTTGTCGCTTTACCGTGAAGTCGAGTTTGATTCGGCGTATACGTTCATTTACTCGCCACGTGAAGGCACCCCAGCGGCG
AAAATGAATGATAACGTGCCGATGGAAGTGAAAAAAGAACGGCTTCAGCGCTTGAACGCCCTCGTTCAAGAGATTGCAGC
GAAGAAAATGAAACAATACGAGGGCCAAGTCGTCGAGGTGCTCGTTGAAGGCGAAAGCAAAACGAACCCGGATGTGTTAG
CAGGGTATACGCGTAAAAACAAGCTTGTCCATTTCGTCGGCCCGAAATCGCTCATTGGTCAATTGGTCAATGTGCGCATT
ACGCAGGCGAAAACGTGGACATTGACAGGCGAGTTAGCGAATGAAGCGATCGAGGTGAACTAA

Upstream 100 bases:

>100_bases
AGAAAAATTCGCCGAGTTAGTAGCGGAGTTTATGTAAAAATGGAAATGATTCACACCCGATGTGGACTTGCCTCATCGGG
TGTTTTTTTGCTATCAAAAC

Downstream 100 bases:

>100_bases
GATGGCAAAATATACGCGGGATGAGATTTTGACGCAGGCAAAGCAGTTGGCGAAAATGATCGCCGAAACAGAGGAAGTTG
ACTTTTTCAAGCGAGCCGAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MFRTSGTKTIVFHCHPRYTMIVCVMEEFCRKKVDGNENNSYLTVCNRKLDQERKEIVMNEKQRLEQTGQIQTASHPADRK
SDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEEVQYVKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIF
MALGYEPTDRPEDANVILLNTCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGT
HNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEV
RQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQ
SGSTEILKMMGRKYTREEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAA
KMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYTRKNKLVHFVGPKSLIGQLVNVRI
TQAKTWTLTGELANEAIEVN

Sequences:

>Translated_580_residues
MFRTSGTKTIVFHCHPRYTMIVCVMEEFCRKKVDGNENNSYLTVCNRKLDQERKEIVMNEKQRLEQTGQIQTASHPADRK
SDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEEVQYVKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIF
MALGYEPTDRPEDANVILLNTCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGT
HNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEV
RQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQ
SGSTEILKMMGRKYTREEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAA
KMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYTRKNKLVHFVGPKSLIGQLVNVRI
TQAKTWTLTGELANEAIEVN
>Mature_580_residues
MFRTSGTKTIVFHCHPRYTMIVCVMEEFCRKKVDGNENNSYLTVCNRKLDQERKEIVMNEKQRLEQTGQIQTASHPADRK
SDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEEVQYVKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIF
MALGYEPTDRPEDANVILLNTCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGT
HNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEV
RQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQ
SGSTEILKMMGRKYTREEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAA
KMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYTRKNKLVHFVGPKSLIGQLVNVRI
TQAKTWTLTGELANEAIEVN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=536, Percent_Identity=36.5671641791045, Blast_Score=316, Evalue=5e-86,
Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=38.1742738589212, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI93277076, Length=478, Percent_Identity=29.9163179916318, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=46.2414578587699, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=26.5033407572383, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=506, Percent_Identity=34.3873517786561, Blast_Score=282, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71996771, Length=313, Percent_Identity=29.073482428115, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21356207, Length=553, Percent_Identity=36.5280289330922, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=29.9781181619256, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 66835; Mature: 66835

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTSGTKTIVFHCHPRYTMIVCVMEEFCRKKVDGNENNSYLTVCNRKLDQERKEIVMNE
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHCH
KQRLEQTGQIQTASHPADRKSDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEEVQY
HHHHHHCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHCCHHHHHH
VKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEDANVILLN
HHHCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
TCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGT
EEECCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECC
HNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCTYCI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHCCCHHEEHHHHHHHHHHHHEEE
VPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELR
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTREEYL
HCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCHHHHH
ELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAA
HHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
KMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYTRKN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCCC
KLVHFVGPKSLIGQLVNVRITQAKTWTLTGELANEAIEVN
CEEEEECCHHHHHHHHCEEEEEEEEEEEEHHHHCCCEECC
>Mature Secondary Structure
MFRTSGTKTIVFHCHPRYTMIVCVMEEFCRKKVDGNENNSYLTVCNRKLDQERKEIVMNE
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHCH
KQRLEQTGQIQTASHPADRKSDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEEVQY
HHHHHHCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHCCHHHHHH
VKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEDANVILLN
HHHCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
TCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGT
EEECCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECC
HNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCTYCI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHCCCHHEEHHHHHHHHHHHHEEE
VPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELR
EECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTREEYL
HCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCHHHHH
ELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAA
HHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
KMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYTRKN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCCC
KLVHFVGPKSLIGQLVNVRITQAKTWTLTGELANEAIEVN
CEEEEECCHHHHHHHHCEEEEEEEEEEEEHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA