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The map label for this gene is truB

Identifier: 138894786

GI number: 138894786

Start: 1182538

End: 1183446

Strand: Direct

Name: truB

Synonym: GTNG_1120

Alternate gene names: 138894786

Gene position: 1182538-1183446 (Clockwise)

Preceding gene: 138894785

Following gene: 138894787

Centisome position: 33.31

GC content: 56.33

Gene sequence:

>909_bases
ATGGACGGGGTATTGCTACTCAATAAACCGAAAGGGATGACGTCGCACGATTGTGTGGCGAAAGTACGACGCCTGCTGGG
CATAAAAAAAGTCGGTCATACCGGCACGCTCGACCCAAACGTGTCTGGTGTGCTGCCGATTTGCCTCGGCAAGGCGACGC
GCATCGCCGAATTTTTAACCGGAGCGACAAAAACATATGAAGGGGAAGTGACACTTGGCGCAGCGACGTCGACCGAAGAC
GCCGATGGTGACATCATCGCCGTCCAGACGGTTGACCGGACGATTGCGCGTGCGGAGATCGAAGCGGTATTCCGCAGCCT
CACTGGGGATATCGAACAAACGCCACCGATGTATTCGGCGGTGAAAGTAAACGGCAAAAAACTGTATGAATATGCGCGTG
CTGGCATCGAAGTCGAACGCCCAACGCGGCGTGTGACCATTTACGAGCTTGAGCTGCTTGATGAACGCGAGCAATTCGTC
GGGGAAACAGTGTCGTTTCGCTTCCGTGTCATGTGCAGCAAAGGCACGTACGTCCGCACGCTCGCTGTCACCATTGGCGA
GCGGCTCGGCTATCCGGCTCATATGTCTGATCTCATCCGCACAGCGTCTGGGCCGTTTCAGCTGGCTGATTGTGTGACGC
TTGAAGAGGTCGAGCGCCGGGCGGCTGACGGGACGGTCGATGCCTTGCTAATTCCAATCGAGCAAGCGCTTTTTCATTTG
CCGAAATACGAAATCAGTGATAAAGTAGCAGAGAAAGTGAAAAACGGGGCGCTGCTTCGTCTCCCCGCCTTTTTGCAGGA
GCTTGACGGGCCGGTTCTGCTCGTGACGCCCGAGCGGGAGGCGATGGCCCTGTACGTGAAGCATCCGGCCCGCCCCGGTG
TGATGAAGCCGTTGAAAGTGTTTCGTTAA

Upstream 100 bases:

>100_bases
ACGGCCGCGATTCCGAAGAGGTGAACGATGAACCGAAAAATGGATAGACGAATCGTCTATCCATTGCCATTAGTGAGTAT
GACACGGAAAGGGTGCGCCG

Downstream 100 bases:

>100_bases
TGCCGGGCGAAATGCGGAAATAAAGAAGGTGGATCATGGACATGGAAACAATATTTATTTCGCATCCGCACCACTTTGAA
CGCCAAGCGCTTCCCCCGAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDGVLLLNKPKGMTSHDCVAKVRRLLGIKKVGHTGTLDPNVSGVLPICLGKATRIAEFLTGATKTYEGEVTLGAATSTED
ADGDIIAVQTVDRTIARAEIEAVFRSLTGDIEQTPPMYSAVKVNGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFV
GETVSFRFRVMCSKGTYVRTLAVTIGERLGYPAHMSDLIRTASGPFQLADCVTLEEVERRAADGTVDALLIPIEQALFHL
PKYEISDKVAEKVKNGALLRLPAFLQELDGPVLLVTPEREAMALYVKHPARPGVMKPLKVFR

Sequences:

>Translated_302_residues
MDGVLLLNKPKGMTSHDCVAKVRRLLGIKKVGHTGTLDPNVSGVLPICLGKATRIAEFLTGATKTYEGEVTLGAATSTED
ADGDIIAVQTVDRTIARAEIEAVFRSLTGDIEQTPPMYSAVKVNGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFV
GETVSFRFRVMCSKGTYVRTLAVTIGERLGYPAHMSDLIRTASGPFQLADCVTLEEVERRAADGTVDALLIPIEQALFHL
PKYEISDKVAEKVKNGALLRLPAFLQELDGPVLLVTPEREAMALYVKHPARPGVMKPLKVFR
>Mature_302_residues
MDGVLLLNKPKGMTSHDCVAKVRRLLGIKKVGHTGTLDPNVSGVLPICLGKATRIAEFLTGATKTYEGEVTLGAATSTED
ADGDIIAVQTVDRTIARAEIEAVFRSLTGDIEQTPPMYSAVKVNGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFV
GETVSFRFRVMCSKGTYVRTLAVTIGERLGYPAHMSDLIRTASGPFQLADCVTLEEVERRAADGTVDALLIPIEQALFHL
PKYEISDKVAEKVKNGALLRLPAFLQELDGPVLLVTPEREAMALYVKHPARPGVMKPLKVFR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=256, Percent_Identity=32.8125, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4503337, Length=298, Percent_Identity=28.8590604026846, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI215599015, Length=298, Percent_Identity=28.8590604026846, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI2367200, Length=269, Percent_Identity=40.1486988847584, Blast_Score=187, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=298, Percent_Identity=27.8523489932886, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=297, Percent_Identity=32.3232323232323, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=32.3076923076923, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281364189, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281364187, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281364185, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281364183, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI62471759, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17975520, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_GEOTN (A4IME0)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125239.1
- ProteinModelPortal:   A4IME0
- SMR:   A4IME0
- STRING:   A4IME0
- GeneID:   4966061
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1120
- NMPDR:   fig|420246.5.peg.1088
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A4IME0
- ProtClustDB:   PRK01550
- BioCyc:   GTHE420246:GTNG_1120-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 33211; Mature: 33211

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVLLLNKPKGMTSHDCVAKVRRLLGIKKVGHTGTLDPNVSGVLPICLGKATRIAEFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHH
GATKTYEGEVTLGAATSTEDADGDIIAVQTVDRTIARAEIEAVFRSLTGDIEQTPPMYSA
CCCEEECCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VKVNGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFVGETVSFRFRVMCSKGTYVRT
EEECHHHHHHHHHCCCEEECCCCEEEEEEEEHHHHHHHHHCCCEEEEEEEEECCCCEEEH
LAVTIGERLGYPAHMSDLIRTASGPFQLADCVTLEEVERRAADGTVDALLIPIEQALFHL
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHC
PKYEISDKVAEKVKNGALLRLPAFLQELDGPVLLVTPEREAMALYVKHPARPGVMKPLKV
CCCCHHHHHHHHHHCCCEEEHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHH
FR
CC
>Mature Secondary Structure
MDGVLLLNKPKGMTSHDCVAKVRRLLGIKKVGHTGTLDPNVSGVLPICLGKATRIAEFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHH
GATKTYEGEVTLGAATSTEDADGDIIAVQTVDRTIARAEIEAVFRSLTGDIEQTPPMYSA
CCCEEECCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VKVNGKKLYEYARAGIEVERPTRRVTIYELELLDEREQFVGETVSFRFRVMCSKGTYVRT
EEECHHHHHHHHHCCCEEECCCCEEEEEEEEHHHHHHHHHCCCEEEEEEEEECCCCEEEH
LAVTIGERLGYPAHMSDLIRTASGPFQLADCVTLEEVERRAADGTVDALLIPIEQALFHL
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHC
PKYEISDKVAEKVKNGALLRLPAFLQELDGPVLLVTPEREAMALYVKHPARPGVMKPLKV
CCCCHHHHHHHHHHCCCEEEHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHH
FR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA