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The map label for this gene is proS [H]

Identifier: 138894777

GI number: 138894777

Start: 1171023

End: 1172726

Strand: Direct

Name: proS [H]

Synonym: GTNG_1111

Alternate gene names: 138894777

Gene position: 1171023-1172726 (Clockwise)

Preceding gene: 138894776

Following gene: 138894778

Centisome position: 32.98

GC content: 53.46

Gene sequence:

>1704_bases
ATGAGACAAAGTCAAGGGTTTATTCCGACATTGCGCGAAGTGCCGGCGGACGCGGAAGTGAAAAGCCATCAGCTCCTGTT
GCGGGCCGGCTTCGTCCGCCAAAGCGCAAGCGGCGTCTACACGTTTTTGCCGCTCGGGCAACGTGTTTTGCAAAAAGTGG
AAGCGATTATTCGTGAGGAGATGAATCGCGCCGGAGCATTGGAGCTTCTCATGCCTGCTTTGCAGCCGGCTGAGCTTTGG
CAGCAGTCCGGGCGCTGGTATTCGTATGGACCGGAGCTCATGCGCCTGAAAGACCGCCACGAGCGCGATTTCGTTCTCGG
ACCGACACACGAAGAGATGATTACTACGATCGTTCGCGATGAAGTGAAAACGTATAAGCGGCTGCCGCTTATCTTGTATC
AAATTCAAACGAAATTCCGTGATGAAAAACGTCCGCGTTTCGGGCTGTTGCGCGGTCGCGAGTTCATCATGAAAGATGCA
TATTCATTCCACACATCGCAGGAAAGTTTGGACGAAACGTACAATAAAATGTATGAAGCGTACGCGAACATTTTCCGCCG
CTGCGGCTTAAATTTCCGCGCTGTCATTGCTGACTCCGGAGCGATGGGCGGCAAAGATACGCACGAGTTTATGGTGCTGT
CTGATATTGGCGAGGATACGATCGCTTATTCCGATGCGTCCGACTATGCGGCCAACATTGAAATGGCACCGGTCGTCACT
ACGTATGAAAAAAGCAGTGAGCCGCTGGTGGAACTGAAAAAAGTGGCGACCCCGGAGCAAAAAACGATTGCTGAAGTTGC
TTCGTATTTGCAAGTAGCACCGGAACGTTGCATTAAATCGCTTTTATTTAACGTTGATGGCCGCTACGTGCTCGTTCTGG
TGCGCGGCGATCATGAAGCGAATGATGTGAAAGTGAAAAATGTGCTTGATGCGACTGTCGTGGAGCTGGCGACACCGGAA
GAAACAGAACGAGTGATGAACTGCCCGGTTGGTTCGCTCGGCCCGATTGGCGTCAGCGAAGAGGTGACGATTATCGCCGA
TCATGCTGTCGCGGCGATCGTAAACGGCGTCTGCGGCGCCAATGAGGAAGGATACCACTATACGGGTGTCAATCCAGACC
GCGATTTTGCCGTCAGTCAATATGCGGATTTGCGTTTCGTCCAAGAAGGCGACCCTTCTCCGGATGGCAACGGGACGATC
CGCTTCGCTCGTGGCATTGAAGTTGGACATGTGTTTAAGCTCGGTACGAAATATAGCGAGGCGATGAACGCCGTTTACCT
CGACGAAAATGGTCGGACACAGACGATGATTATGGGTTGCTACGGCATTGGCGTCTCTAGGCTCGTTGCGGCGATCGCCG
AGCAGTTCGCCGATGAGAACGGGCTTGTATGGCCGGTTTCGGTCGCACCGTTTCACGTTCATTTGCTGACGGCGAACGCG
AAAAGCGATGAACAGCGCATGCTGGCTGAAGAGTGGTACGAAAAACTCGGACAGGCCGGATTTGACGTGTTGTATGATGA
CCGTCCGGAACGAGCCGGGGTGAAGTTTGCCGACAGCGATTTGATCGGCATCCCGCTCCGCGTCACCGTTGGCAAGCGGG
CAAGTGAAGGTGTGGTCGAAGTAAAAGTTCGGAAAACAGGCGAGACGTTTGACGTGCCGGTCGGTGAGCTGATCGAAACA
GTGCGCCGTCTTTTGCAAGGATAA

Upstream 100 bases:

>100_bases
ATCGGCTTCGCCTTGCTTATGTTACTTATGCTTGTCGTGACATGGAATGACATTCAAAAATTCTTCCTATAATGAAAGAG
AGAAAACGAGGTGGCTATTG

Downstream 100 bases:

>100_bases
ACGAACGGCGCCGGCGAAAAAGAACCCGCTTCTTTTTTCGCCGGCGCTGTTGCCGTTTGTATGGTATGATAGAGAAAGAG
AAATGATTTCGTTGAGAGGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MRQSQGFIPTLREVPADAEVKSHQLLLRAGFVRQSASGVYTFLPLGQRVLQKVEAIIREEMNRAGALELLMPALQPAELW
QQSGRWYSYGPELMRLKDRHERDFVLGPTHEEMITTIVRDEVKTYKRLPLILYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHTSQESLDETYNKMYEAYANIFRRCGLNFRAVIADSGAMGGKDTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVVT
TYEKSSEPLVELKKVATPEQKTIAEVASYLQVAPERCIKSLLFNVDGRYVLVLVRGDHEANDVKVKNVLDATVVELATPE
ETERVMNCPVGSLGPIGVSEEVTIIADHAVAAIVNGVCGANEEGYHYTGVNPDRDFAVSQYADLRFVQEGDPSPDGNGTI
RFARGIEVGHVFKLGTKYSEAMNAVYLDENGRTQTMIMGCYGIGVSRLVAAIAEQFADENGLVWPVSVAPFHVHLLTANA
KSDEQRMLAEEWYEKLGQAGFDVLYDDRPERAGVKFADSDLIGIPLRVTVGKRASEGVVEVKVRKTGETFDVPVGELIET
VRRLLQG

Sequences:

>Translated_567_residues
MRQSQGFIPTLREVPADAEVKSHQLLLRAGFVRQSASGVYTFLPLGQRVLQKVEAIIREEMNRAGALELLMPALQPAELW
QQSGRWYSYGPELMRLKDRHERDFVLGPTHEEMITTIVRDEVKTYKRLPLILYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHTSQESLDETYNKMYEAYANIFRRCGLNFRAVIADSGAMGGKDTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVVT
TYEKSSEPLVELKKVATPEQKTIAEVASYLQVAPERCIKSLLFNVDGRYVLVLVRGDHEANDVKVKNVLDATVVELATPE
ETERVMNCPVGSLGPIGVSEEVTIIADHAVAAIVNGVCGANEEGYHYTGVNPDRDFAVSQYADLRFVQEGDPSPDGNGTI
RFARGIEVGHVFKLGTKYSEAMNAVYLDENGRTQTMIMGCYGIGVSRLVAAIAEQFADENGLVWPVSVAPFHVHLLTANA
KSDEQRMLAEEWYEKLGQAGFDVLYDDRPERAGVKFADSDLIGIPLRVTVGKRASEGVVEVKVRKTGETFDVPVGELIET
VRRLLQG
>Mature_567_residues
MRQSQGFIPTLREVPADAEVKSHQLLLRAGFVRQSASGVYTFLPLGQRVLQKVEAIIREEMNRAGALELLMPALQPAELW
QQSGRWYSYGPELMRLKDRHERDFVLGPTHEEMITTIVRDEVKTYKRLPLILYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHTSQESLDETYNKMYEAYANIFRRCGLNFRAVIADSGAMGGKDTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVVT
TYEKSSEPLVELKKVATPEQKTIAEVASYLQVAPERCIKSLLFNVDGRYVLVLVRGDHEANDVKVKNVLDATVVELATPE
ETERVMNCPVGSLGPIGVSEEVTIIADHAVAAIVNGVCGANEEGYHYTGVNPDRDFAVSQYADLRFVQEGDPSPDGNGTI
RFARGIEVGHVFKLGTKYSEAMNAVYLDENGRTQTMIMGCYGIGVSRLVAAIAEQFADENGLVWPVSVAPFHVHLLTANA
KSDEQRMLAEEWYEKLGQAGFDVLYDDRPERAGVKFADSDLIGIPLRVTVGKRASEGVVEVKVRKTGETFDVPVGELIET
VRRLLQG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=45.575221238938, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=53.4843205574913, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI115532348, Length=230, Percent_Identity=39.5652173913043, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=40, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=553, Percent_Identity=31.1030741410488, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24656200, Length=256, Percent_Identity=35.9375, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63276; Mature: 63276

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSQGFIPTLREVPADAEVKSHQLLLRAGFVRQSASGVYTFLPLGQRVLQKVEAIIREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
MNRAGALELLMPALQPAELWQQSGRWYSYGPELMRLKDRHERDFVLGPTHEEMITTIVRD
HHHCCHHHHHHHCCCHHHHHHHCCCEEECCHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
EVKTYKRLPLILYQIQTKFRDEKRPRFGLLRGREFIMKDAYSFHTSQESLDETYNKMYEA
HHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YANIFRRCGLNFRAVIADSGAMGGKDTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVVT
HHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEECCCCCHHCCCEECCEEE
TYEKSSEPLVELKKVATPEQKTIAEVASYLQVAPERCIKSLLFNVDGRYVLVLVRGDHEA
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NDVKVKNVLDATVVELATPEETERVMNCPVGSLGPIGVSEEVTIIADHAVAAIVNGVCGA
CCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCC
NEEGYHYTGVNPDRDFAVSQYADLRFVQEGDPSPDGNGTIRFARGIEVGHVFKLGTKYSE
CCCCCEECCCCCCCCHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCEEECCCHHHH
AMNAVYLDENGRTQTMIMGCYGIGVSRLVAAIAEQFADENGLVWPVSVAPFHVHLLTANA
HCCEEEECCCCCEEEEEEEEHHHCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEECC
KSDEQRMLAEEWYEKLGQAGFDVLYDDRPERAGVKFADSDLIGIPLRVTVGKRASEGVVE
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECCCCCCCEEE
VKVRKTGETFDVPVGELIETVRRLLQG
EEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQSQGFIPTLREVPADAEVKSHQLLLRAGFVRQSASGVYTFLPLGQRVLQKVEAIIREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
MNRAGALELLMPALQPAELWQQSGRWYSYGPELMRLKDRHERDFVLGPTHEEMITTIVRD
HHHCCHHHHHHHCCCHHHHHHHCCCEEECCHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
EVKTYKRLPLILYQIQTKFRDEKRPRFGLLRGREFIMKDAYSFHTSQESLDETYNKMYEA
HHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YANIFRRCGLNFRAVIADSGAMGGKDTHEFMVLSDIGEDTIAYSDASDYAANIEMAPVVT
HHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEECCCCCHHCCCEECCEEE
TYEKSSEPLVELKKVATPEQKTIAEVASYLQVAPERCIKSLLFNVDGRYVLVLVRGDHEA
ECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NDVKVKNVLDATVVELATPEETERVMNCPVGSLGPIGVSEEVTIIADHAVAAIVNGVCGA
CCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCC
NEEGYHYTGVNPDRDFAVSQYADLRFVQEGDPSPDGNGTIRFARGIEVGHVFKLGTKYSE
CCCCCEECCCCCCCCHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCEEECCCHHHH
AMNAVYLDENGRTQTMIMGCYGIGVSRLVAAIAEQFADENGLVWPVSVAPFHVHLLTANA
HCCEEEECCCCCEEEEEEEEHHHCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEECC
KSDEQRMLAEEWYEKLGQAGFDVLYDDRPERAGVKFADSDLIGIPLRVTVGKRASEGVVE
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECCCCCCCEEE
VKVRKTGETFDVPVGELIETVRRLLQG
EEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA