| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
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The map label for this gene is mtnD [H]
Identifier: 138894514
GI number: 138894514
Start: 919221
End: 919763
Strand: Direct
Name: mtnD [H]
Synonym: GTNG_0844
Alternate gene names: 138894514
Gene position: 919221-919763 (Clockwise)
Preceding gene: 138894513
Following gene: 138894516
Centisome position: 25.89
GC content: 45.67
Gene sequence:
>543_bases ATGGCAGTGATTAAAGTGAAAAAAACGGGCCAAGTGATTGAAGGGGAAGACAATGTCCGCGCTTTTTTAAACAGCCAAGG TGTTTTGTATGAGCATTGGGATATTACAAAACTACCGGAACATTTACGCGACAAATATGTGCTCACTGACGAAGAAAAAG AGGAAATTTTGGCGACGTTTAAAGATGAGATCGAAGACTTGGCGGCGCGTCGTGGCTACAAAACATGGGATATCGTCGCC CTGTCTGATGCGACTCCAAACTTGGAAGAGCTGCTGAAAAAATTTGAGCAAGTGCATATCCACACAGAGGATGAAGTGCG CGCCATTACGGCCGGTCACGGCATTTTCATCATCAAAGGAGACAAAGAAACCGGTTATTTTGACGTCGAGTTGGAAGCGG GCGATGTCATTTCTGTGCCGGAAGGAAATCCGCATTACTTCACATTGATGGATGACCGCCAAGTTGTTGCTGTCCGATTG TTTATTGATCCGTCCGGTTGGGTCGCCCATCCGTACGAAGAAAAAGAAGAAGTGAATTCATAA
Upstream 100 bases:
>100_bases GGGAGTTTTTGTTCAGCTGGCAGGTGAAGCGGCTATTATTACAACGGGCCGCTGTGCCGCTAGTTGACTAACCGATATAT TTTTAAAGGGGGATTTGACG
Downstream 100 bases:
>100_bases TAGTTGAGAGGCTCTCGCAACAAGGTGCCTTTTCTAAGTTATCTAGGTAGACATGGAAAACGCCTGCTTTCCATCGTCTT CATAAAAGCGCATATAAAAA
Product: 5-methylthio-3-oxo-1-penten-1,2-diol dioxygenase
Products: NA
Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD [H]
Number of amino acids: Translated: 180; Mature: 179
Protein sequence:
>180_residues MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVA LSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRL FIDPSGWVAHPYEEKEEVNS
Sequences:
>Translated_180_residues MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVA LSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRL FIDPSGWVAHPYEEKEEVNS >Mature_179_residues AVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATFKDEIEDLAARRGYKTWDIVAL SDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLF IDPSGWVAHPYEEKEEVNS
Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me
COG id: COG1791
COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the acireductone dioxygenase (ARD) family [H]
Homologues:
Organism=Homo sapiens, GI171460928, Length=109, Percent_Identity=35.7798165137615, Blast_Score=74, Evalue=9e-14, Organism=Saccharomyces cerevisiae, GI6323651, Length=113, Percent_Identity=30.0884955752212, Blast_Score=62, Evalue=5e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004313 - InterPro: IPR011051 - InterPro: IPR014710 [H]
Pfam domain/function: PF03079 ARD [H]
EC number: =1.13.11.54; =1.13.11.53 [H]
Molecular weight: Translated: 20597; Mature: 20466
Theoretical pI: Translated: 4.42; Mature: 4.42
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.1 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 0.6 %Met (Mature Protein) 0.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATF CEEEEEECCCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCEEECCCHHHHHHHHH KDEIEDLAARRGYKTWDIVALSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKG HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHEECCCCCCEEEEEECCEEEEEEC DKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLFIDPSGWVAHPYEEKEEVNS CCCCCEEEEEECCCCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCEECCCCCHHHCCC >Mature Secondary Structure AVIKVKKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKEEILATF EEEEEECCCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCEEECCCHHHHHHHHH KDEIEDLAARRGYKTWDIVALSDATPNLEELLKKFEQVHIHTEDEVRAITAGHGIFIIKG HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHEECCCCCCEEEEEECCEEEEEEC DKETGYFDVELEAGDVISVPEGNPHYFTLMDDRQVVAVRLFIDPSGWVAHPYEEKEEVNS CCCCCEEEEEECCCCEEECCCCCCEEEEEECCCEEEEEEEEECCCCCEECCCCCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA