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The map label for this gene is ogt [H]

Identifier: 138894503

GI number: 138894503

Start: 907408

End: 907899

Strand: Direct

Name: ogt [H]

Synonym: GTNG_0833

Alternate gene names: 138894503

Gene position: 907408-907899 (Clockwise)

Preceding gene: 138894502

Following gene: 138894510

Centisome position: 25.56

GC content: 53.46

Gene sequence:

>492_bases
ATGACAATTGACTATGCGGTATACCGTTCAGAGTTGCTCGGCGATATCTATATTGCTTCTGATGGCGACGCGATTGTGAA
AGTCGAACTGTTTCCGGAAGAATGGCGCGCGTTTGCAAGCGAGCATCCGGTTGTAGAAAGCCGGTCACCGCTTCTTGAAC
ACGCGCTTGCACAGTTGGATGAATATTTCCACGGTCGCCGCCAAACGTTTGATTTTCCAGTTAAATGGCATGGGACGGTT
TTCCAACAGGAGGTGTGGACGGCGCTTTGTCGCATTCCATATGGAGAAACGGCGACATACGCCGATATTGCGATGCAGAT
TGGCCGCCCGAAAGCGGTACGCGCCGTCGGACAGGCGAATCGGGCGAATGAACTAGCCATTGTTGTTCCGTGCCATCGTG
TGATCGGCAAAAACGGCGCCTTGACCGGCTATGCTGGAAGCCGCACCGATGTGAAAGCGAAGCTATTGGAGCTGGAGCGG
CGCTGTAAATAG

Upstream 100 bases:

>100_bases
GAAGAACACCGAGGGAAAATTGACGTCGAAAGCGAAGTTGGCGTCGGCACGACATTTCATATTACGCTGCCGATCGAACG
AACGGAAAGAGAGGATAACG

Downstream 100 bases:

>100_bases
GGCGGAAGGGCCGGGGATGAAGCATGGAAAATAAAAAATGCCGCCCCCGGGACTTGAACCTACAGGGGCGGGATCGGGGG
CTATTTTTTTTGCCAATGTG

Product: O6-methylguanine DNA alkyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTIDYAVYRSELLGDIYIASDGDAIVKVELFPEEWRAFASEHPVVESRSPLLEHALAQLDEYFHGRRQTFDFPVKWHGTV
FQQEVWTALCRIPYGETATYADIAMQIGRPKAVRAVGQANRANELAIVVPCHRVIGKNGALTGYAGSRTDVKAKLLELER
RCK

Sequences:

>Translated_163_residues
MTIDYAVYRSELLGDIYIASDGDAIVKVELFPEEWRAFASEHPVVESRSPLLEHALAQLDEYFHGRRQTFDFPVKWHGTV
FQQEVWTALCRIPYGETATYADIAMQIGRPKAVRAVGQANRANELAIVVPCHRVIGKNGALTGYAGSRTDVKAKLLELER
RCK
>Mature_162_residues
TIDYAVYRSELLGDIYIASDGDAIVKVELFPEEWRAFASEHPVVESRSPLLEHALAQLDEYFHGRRQTFDFPVKWHGTVF
QQEVWTALCRIPYGETATYADIAMQIGRPKAVRAVGQANRANELAIVVPCHRVIGKNGALTGYAGSRTDVKAKLLELERR
CK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=111, Percent_Identity=39.6396396396396, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1787596, Length=91, Percent_Identity=53.8461538461538, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1788542, Length=97, Percent_Identity=48.4536082474227, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=48.780487804878, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=48.780487804878, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=48.1927710843374, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137554, Length=89, Percent_Identity=44.9438202247191, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18364; Mature: 18233

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDYAVYRSELLGDIYIASDGDAIVKVELFPEEWRAFASEHPVVESRSPLLEHALAQLD
CCCCHHHHHHHHHCEEEEECCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EYFHGRRQTFDFPVKWHGTVFQQEVWTALCRIPYGETATYADIAMQIGRPKAVRAVGQAN
HHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHCCC
RANELAIVVPCHRVIGKNGALTGYAGSRTDVKAKLLELERRCK
CCCCEEEEEECHHHHCCCCCEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDYAVYRSELLGDIYIASDGDAIVKVELFPEEWRAFASEHPVVESRSPLLEHALAQLD
CCCHHHHHHHHHCEEEEECCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EYFHGRRQTFDFPVKWHGTVFQQEVWTALCRIPYGETATYADIAMQIGRPKAVRAVGQAN
HHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHCCC
RANELAIVVPCHRVIGKNGALTGYAGSRTDVKAKLLELERRCK
CCCCEEEEEECHHHHCCCCCEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]