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The map label for this gene is kinE [H]

Identifier: 138894502

GI number: 138894502

Start: 905202

End: 907418

Strand: Direct

Name: kinE [H]

Synonym: GTNG_0832

Alternate gene names: 138894502

Gene position: 905202-907418 (Clockwise)

Preceding gene: 138894500

Following gene: 138894503

Centisome position: 25.5

GC content: 46.73

Gene sequence:

>2217_bases
ATGGCCCAAAGCCTTTCTCCCCGTGAAGAACAAAATTCGACACAAGATGTGCGGTTGCTTGAAGAGGAAAACCGACGCTT
AAAAGAACGGTTGAACAGCTATTCGATCATTTTTGAGAGGTCGCTTGATGCGATTGTCATTTTAAATAAAAACGGCGAGT
TTGTCGATGTGAACGAAGCAGCGTGCAAGCTGTTTGAAATGGACAAGTTTGACTTGATTGGTCGTTCCTTCTCTTCATTT
TTAGAGCTCGTTCCGCCGGATATTTTGTTGTTTCAGCGCTCCATGCTACAAAAGTTCGGTTCGTTTAGCGATGAATTGCT
CATCAAGTTGCCGGATGACAAGGTGAAACATGTTGAATTTTCGATGAAAAAGGATGTCTTTAACGAATTTGATTTGGTGA
TGATGCGCGACGTTTCGGCTCATAAGGCGCTCGAGCGCGAGCGAATCATTCATGAATTTTTGTTTCGAGACGTCTTTAAC
CGAGCGGTCGATGGCATCGTCATTTTCGATGAGTTCGGTCGGTTTATCGACGTCAATCCGGCATTTTCAATGAGCTTGAA
TTTGGAAAAAGGAAAGCTGCTGAATTTGTCGTTTCAGCAGTTTGTTGCCCGCGAATGTGCTGATGAGTTTGCCCGTCTGT
TGTCGGAAGTGAAGGAAAAAGGAACGGCCAAAGGCGAGCTGTCGTTGGTTCGCCTTGACGGAACGGTGAAAATGTTTGAG
CTGACGGCAACGTCGAACGTTTACAGCGGCTTTTACATGGCCATTATGCGCGATGTGACTGAACGGAAAAACATGGAGAT
TAAATTGCAAAAAAGTGAAGAACGATTCCGGGCCATTTTCGAGCAAGCGCATGAAGCGATTATCATTTGGGATGATTTCG
GTTACATACTCAACGCCAATCCAGCGGCGAGCCGGACGTTTGAGCTGCCTTTGAACTTGCTTGTACGCCGCAATTTGCTT
GATTTTGTGCAATATGGGGATGAAAAGGTGAAGCGCATTTTCAGCGAGTTCCGGCGCAAAGGAGAAATTCGCGATGAATT
AACGTTCCATATGCCGAACGGTGAGGATAAGCAGCTTGAGTTTACGATGAAAAAAGGGGCGATCGACGGCTATCATTTGA
CGATTTTCCGCAATGTGAGCGAGCGGCGGAAAATCGAGCGCGAGCTGCGTGAAAGTGAGCAAAAGTTCCGCAGCATTTTC
GACCATGCGATGGACGGCATTTTGTTATGGGATGAGAAGCATCGCATCATTGACGCTAATCCAATTGCCTGCGAATTTTT
TTCCGCCACAAAAGAAGCGTTGCTCGGTCAAACGGTGATGGAACTTGTTCCAGAGCGGGCGCGCCGCCAGCTGAATAAGC
TCTTTGTACAATGTGAACGTGCAGGCGAGGCGAGTGGGGAAGTTGAATTTTCCGATGAAGGAAAAGAGAAAATCGTTGAG
TTTTCATTGAAAAAAGAAGTCATTCCTGGCGTAGGGATGATGAGATTTCGCGATGTCACAGAGCGTAAAGAAATGGAGCT
GCAGCTGCGCAAATCGGATACGCTCAATGTTGTTGGTGAATTGGCTGCGGGCATCGCTCACGAAATCCGCAATCCGATGA
CGGCATTAAAAGGCTTTATCCAACTGTTGCAAGGAAGCGTTGGCGGCGATTATTCGATGTATTTTAATGTCATTATGTCG
GAATTGAAGCGGATCGAGTCAATCATCACCGAGTTTCTCGTCTTGGCCAAACCCCAGGCGGTGCAATACAAGCAAAATGA
CATTTGCAAAATTATGCAGGATACGATCGATTTAATCAGCGCTCAGGCGATGATGCATAATGTGCAAATCATTGCCGATT
TCGAGCGCGGTCTGCCTCCGGTGTATTGCGAGCCGAACCAGTTGAAGCAAGTGTTTATCAATATTTTGAAAAACGCCATT
GAAGTGATGCCGAAGGGCGGGGACATTACGGTGCGCATCGCCCGTACTGGACGTCATGTGCGCATTTCAATCACCGACCA
AGGGTGCGGTATTCCGAAAGATAAAATTAAAAAGCTCGGTGAGCCGTTTTATACGACAAAAGAGCGCGGCACTGGACTCG
GCTTGATGGTCAGCTATAAAATTATTGAAGAACACCGAGGGAAAATTGACGTCGAAAGCGAAGTTGGCGTCGGCACGACA
TTTCATATTACGCTGCCGATCGAACGAACGGAAAGAGAGGATAACGATGACAATTGA

Upstream 100 bases:

>100_bases
AAGATTTTTGAAAAATCTTCGTGAATGGAGGAAGGAGTAAATTTTTCCACATAGAATATATATTAGTTGATATGGTTTCA
ATGGACAAGGAGAGATCAAT

Downstream 100 bases:

>100_bases
CTATGCGGTATACCGTTCAGAGTTGCTCGGCGATATCTATATTGCTTCTGATGGCGACGCGATTGTGAAAGTCGAACTGT
TTCCGGAAGAATGGCGCGCG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MAQSLSPREEQNSTQDVRLLEEENRRLKERLNSYSIIFERSLDAIVILNKNGEFVDVNEAACKLFEMDKFDLIGRSFSSF
LELVPPDILLFQRSMLQKFGSFSDELLIKLPDDKVKHVEFSMKKDVFNEFDLVMMRDVSAHKALERERIIHEFLFRDVFN
RAVDGIVIFDEFGRFIDVNPAFSMSLNLEKGKLLNLSFQQFVARECADEFARLLSEVKEKGTAKGELSLVRLDGTVKMFE
LTATSNVYSGFYMAIMRDVTERKNMEIKLQKSEERFRAIFEQAHEAIIIWDDFGYILNANPAASRTFELPLNLLVRRNLL
DFVQYGDEKVKRIFSEFRRKGEIRDELTFHMPNGEDKQLEFTMKKGAIDGYHLTIFRNVSERRKIERELRESEQKFRSIF
DHAMDGILLWDEKHRIIDANPIACEFFSATKEALLGQTVMELVPERARRQLNKLFVQCERAGEASGEVEFSDEGKEKIVE
FSLKKEVIPGVGMMRFRDVTERKEMELQLRKSDTLNVVGELAAGIAHEIRNPMTALKGFIQLLQGSVGGDYSMYFNVIMS
ELKRIESIITEFLVLAKPQAVQYKQNDICKIMQDTIDLISAQAMMHNVQIIADFERGLPPVYCEPNQLKQVFINILKNAI
EVMPKGGDITVRIARTGRHVRISITDQGCGIPKDKIKKLGEPFYTTKERGTGLGLMVSYKIIEEHRGKIDVESEVGVGTT
FHITLPIERTEREDNDDN

Sequences:

>Translated_738_residues
MAQSLSPREEQNSTQDVRLLEEENRRLKERLNSYSIIFERSLDAIVILNKNGEFVDVNEAACKLFEMDKFDLIGRSFSSF
LELVPPDILLFQRSMLQKFGSFSDELLIKLPDDKVKHVEFSMKKDVFNEFDLVMMRDVSAHKALERERIIHEFLFRDVFN
RAVDGIVIFDEFGRFIDVNPAFSMSLNLEKGKLLNLSFQQFVARECADEFARLLSEVKEKGTAKGELSLVRLDGTVKMFE
LTATSNVYSGFYMAIMRDVTERKNMEIKLQKSEERFRAIFEQAHEAIIIWDDFGYILNANPAASRTFELPLNLLVRRNLL
DFVQYGDEKVKRIFSEFRRKGEIRDELTFHMPNGEDKQLEFTMKKGAIDGYHLTIFRNVSERRKIERELRESEQKFRSIF
DHAMDGILLWDEKHRIIDANPIACEFFSATKEALLGQTVMELVPERARRQLNKLFVQCERAGEASGEVEFSDEGKEKIVE
FSLKKEVIPGVGMMRFRDVTERKEMELQLRKSDTLNVVGELAAGIAHEIRNPMTALKGFIQLLQGSVGGDYSMYFNVIMS
ELKRIESIITEFLVLAKPQAVQYKQNDICKIMQDTIDLISAQAMMHNVQIIADFERGLPPVYCEPNQLKQVFINILKNAI
EVMPKGGDITVRIARTGRHVRISITDQGCGIPKDKIKKLGEPFYTTKERGTGLGLMVSYKIIEEHRGKIDVESEVGVGTT
FHITLPIERTEREDNDDN
>Mature_737_residues
AQSLSPREEQNSTQDVRLLEEENRRLKERLNSYSIIFERSLDAIVILNKNGEFVDVNEAACKLFEMDKFDLIGRSFSSFL
ELVPPDILLFQRSMLQKFGSFSDELLIKLPDDKVKHVEFSMKKDVFNEFDLVMMRDVSAHKALERERIIHEFLFRDVFNR
AVDGIVIFDEFGRFIDVNPAFSMSLNLEKGKLLNLSFQQFVARECADEFARLLSEVKEKGTAKGELSLVRLDGTVKMFEL
TATSNVYSGFYMAIMRDVTERKNMEIKLQKSEERFRAIFEQAHEAIIIWDDFGYILNANPAASRTFELPLNLLVRRNLLD
FVQYGDEKVKRIFSEFRRKGEIRDELTFHMPNGEDKQLEFTMKKGAIDGYHLTIFRNVSERRKIERELRESEQKFRSIFD
HAMDGILLWDEKHRIIDANPIACEFFSATKEALLGQTVMELVPERARRQLNKLFVQCERAGEASGEVEFSDEGKEKIVEF
SLKKEVIPGVGMMRFRDVTERKEMELQLRKSDTLNVVGELAAGIAHEIRNPMTALKGFIQLLQGSVGGDYSMYFNVIMSE
LKRIESIITEFLVLAKPQAVQYKQNDICKIMQDTIDLISAQAMMHNVQIIADFERGLPPVYCEPNQLKQVFINILKNAIE
VMPKGGDITVRIARTGRHVRISITDQGCGIPKDKIKKLGEPFYTTKERGTGLGLMVSYKIIEEHRGKIDVESEVGVGTTF
HITLPIERTEREDNDDN

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=352, Percent_Identity=30.9659090909091, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=33.4728033472803, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=29.3447293447293, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786600, Length=390, Percent_Identity=24.6153846153846, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788713, Length=279, Percent_Identity=25.8064516129032, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790346, Length=216, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=262, Percent_Identity=27.0992366412214, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788393, Length=224, Percent_Identity=24.1071428571429, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=26.3392857142857, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=221, Percent_Identity=24.8868778280543, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=26.4227642276423, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=22.7678571428571, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=27.5109170305677, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=238, Percent_Identity=22.2689075630252, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85089; Mature: 84958

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSLSPREEQNSTQDVRLLEEENRRLKERLNSYSIIFERSLDAIVILNKNGEFVDVNEA
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEECCHH
ACKLFEMDKFDLIGRSFSSFLELVPPDILLFQRSMLQKFGSFSDELLIKLPDDKVKHVEF
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
SMKKDVFNEFDLVMMRDVSAHKALERERIIHEFLFRDVFNRAVDGIVIFDEFGRFIDVNP
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
AFSMSLNLEKGKLLNLSFQQFVARECADEFARLLSEVKEKGTAKGELSLVRLDGTVKMFE
CEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEE
LTATSNVYSGFYMAIMRDVTERKNMEIKLQKSEERFRAIFEQAHEAIIIWDDFGYILNAN
EEECCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCEEEECC
PAASRTFELPLNLLVRRNLLDFVQYGDEKVKRIFSEFRRKGEIRDELTFHMPNGEDKQLE
CCCCCEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEE
FTMKKGAIDGYHLTIFRNVSERRKIERELRESEQKFRSIFDHAMDGILLWDEKHRIIDAN
EEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECC
PIACEFFSATKEALLGQTVMELVPERARRQLNKLFVQCERAGEASGEVEFSDEGKEKIVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
FSLKKEVIPGVGMMRFRDVTERKEMELQLRKSDTLNVVGELAAGIAHEIRNPMTALKGFI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
QLLQGSVGGDYSMYFNVIMSELKRIESIITEFLVLAKPQAVQYKQNDICKIMQDTIDLIS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHH
AQAMMHNVQIIADFERGLPPVYCEPNQLKQVFINILKNAIEVMPKGGDITVRIARTGRHV
HHHHHHHHHEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEE
RISITDQGCGIPKDKIKKLGEPFYTTKERGTGLGLMVSYKIIEEHRGKIDVESEVGVGTT
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEECCCCCCCEE
FHITLPIERTEREDNDDN
EEEEEECHHCCCCCCCCC
>Mature Secondary Structure 
AQSLSPREEQNSTQDVRLLEEENRRLKERLNSYSIIFERSLDAIVILNKNGEFVDVNEA
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEECCHH
ACKLFEMDKFDLIGRSFSSFLELVPPDILLFQRSMLQKFGSFSDELLIKLPDDKVKHVEF
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
SMKKDVFNEFDLVMMRDVSAHKALERERIIHEFLFRDVFNRAVDGIVIFDEFGRFIDVNP
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
AFSMSLNLEKGKLLNLSFQQFVARECADEFARLLSEVKEKGTAKGELSLVRLDGTVKMFE
CEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEE
LTATSNVYSGFYMAIMRDVTERKNMEIKLQKSEERFRAIFEQAHEAIIIWDDFGYILNAN
EEECCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCEEEECC
PAASRTFELPLNLLVRRNLLDFVQYGDEKVKRIFSEFRRKGEIRDELTFHMPNGEDKQLE
CCCCCEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEE
FTMKKGAIDGYHLTIFRNVSERRKIERELRESEQKFRSIFDHAMDGILLWDEKHRIIDAN
EEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECC
PIACEFFSATKEALLGQTVMELVPERARRQLNKLFVQCERAGEASGEVEFSDEGKEKIVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
FSLKKEVIPGVGMMRFRDVTERKEMELQLRKSDTLNVVGELAAGIAHEIRNPMTALKGFI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
QLLQGSVGGDYSMYFNVIMSELKRIESIITEFLVLAKPQAVQYKQNDICKIMQDTIDLIS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHH
AQAMMHNVQIIADFERGLPPVYCEPNQLKQVFINILKNAIEVMPKGGDITVRIARTGRHV
HHHHHHHHHEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEE
RISITDQGCGIPKDKIKKLGEPFYTTKERGTGLGLMVSYKIIEEHRGKIDVESEVGVGTT
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEECCCCCCCEE
FHITLPIERTEREDNDDN
EEEEEECHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]