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The map label for this gene is cysK [H]

Identifier: 138893744

GI number: 138893744

Start: 77655

End: 78581

Strand: Direct

Name: cysK [H]

Synonym: GTNG_0065

Alternate gene names: 138893744

Gene position: 77655-78581 (Clockwise)

Preceding gene: 138893743

Following gene: 138893745

Centisome position: 2.19

GC content: 51.78

Gene sequence:

>927_bases
ATGGCACGCACAGTCAACTCTGTAACGGAACTGATCGGCGATACACCGGCAGTGAAGTTGAACCGCATCGTCGACGAAGA
CAGCGCTGATGTTTACGTGAAGCTCGAGTTTATGAACCCAGGCAGCAGCGTCAAAGACCGGATTGCATTAGCAATGATTG
AGGCAGCGGAAAAGGCCGGAAGACTGAAACCGGGCGATACAATCGTTGAGCCGACGAGCGGTAACACGGGAATCGGATTA
GCGATGGTCGCAGCAGCGAAAGGATATAAAGCAGTATTGGTTATGCCGGATACGATGAGTTTAGAGCGCCGCAACTTGTT
GCGGGCGTACGGTGCAGAGTTAGTACTCACGCCAGGTGCTCAAGGGATGCGTGGGGCAATCCAAAAAGCTGAAGAACTCG
CTCGCGAGCACGGCTACTTTATGCCGCAGCAGTTCAAAAATGAAGCAAACCCAGAAATCCACCGTTTGACGACCGGGAAA
GAAATTGTTGAGCAAATGGGCGACCAGCTCGATGCGTTTGTTGCTGGAATCGGTACGGGCGGAACGATTACGGGCGCTGG
TAAAGTATTGCGTGAAGCATATCCGAACATTAAAATTTACGCCGTTGAGCCGGCTGATTCACCGGTCTTGTCCGGCGGTA
AACCAGGTCCGCATAAAATCCAAGGAATCGGCGCTGGGTTTGTTCCAGATATTTTGGATACAAGCATTTATGACGGCGTC
ATTACGGTAACAACGGAAGAAGCGTTTGCGGCTGCCCGCCGCGCGGCTCGCGAGGAAGGCATTCTTGGCGGCATTTCGTC
TGGTGCTGCTATTCATGCGGCGTTGAAAGTAGCAAAAGAACTCGGTAAAGGAAAAAAAGTGCTGGCTGTGATCCCGAGCA
ATGGGGAACGTTATTTGAGCACGCCGCTTTACCAATTCGAAAACTAA

Upstream 100 bases:

>100_bases
CCGAAAAAAGATGAAAACATTAAAATGATTGACAATTACGTCAATAACTGATACATTGGAAACAAATTCGATAAAAATAA
TCGGGATTAGGGGTGGAACC

Downstream 100 bases:

>100_bases
GCAACATTTTTCGACAGGGGCAACCCTGTCGAATTTTTTATGGTAGTGGGTGATATGAATGACTAATCATTGTACAATAA
GAATAACTATGAACATCATG

Product: O-acetyl-L-serine sulfhydrylase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MARTVNSVTELIGDTPAVKLNRIVDEDSADVYVKLEFMNPGSSVKDRIALAMIEAAEKAGRLKPGDTIVEPTSGNTGIGL
AMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGAQGMRGAIQKAEELAREHGYFMPQQFKNEANPEIHRLTTGK
EIVEQMGDQLDAFVAGIGTGGTITGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGV
ITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAVIPSNGERYLSTPLYQFEN

Sequences:

>Translated_308_residues
MARTVNSVTELIGDTPAVKLNRIVDEDSADVYVKLEFMNPGSSVKDRIALAMIEAAEKAGRLKPGDTIVEPTSGNTGIGL
AMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGAQGMRGAIQKAEELAREHGYFMPQQFKNEANPEIHRLTTGK
EIVEQMGDQLDAFVAGIGTGGTITGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGV
ITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAVIPSNGERYLSTPLYQFEN
>Mature_307_residues
ARTVNSVTELIGDTPAVKLNRIVDEDSADVYVKLEFMNPGSSVKDRIALAMIEAAEKAGRLKPGDTIVEPTSGNTGIGLA
MVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGAQGMRGAIQKAEELAREHGYFMPQQFKNEANPEIHRLTTGKE
IVEQMGDQLDAFVAGIGTGGTITGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGVI
TVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAVIPSNGERYLSTPLYQFEN

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=303, Percent_Identity=41.2541254125413, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI295821200, Length=303, Percent_Identity=41.2541254125413, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI4557415, Length=303, Percent_Identity=41.2541254125413, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1788754, Length=310, Percent_Identity=55.4838709677419, Blast_Score=286, Evalue=9e-79,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=43.0921052631579, Blast_Score=234, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=299, Percent_Identity=50.5016722408027, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI115535073, Length=306, Percent_Identity=52.9411764705882, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=49.3333333333333, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=49.6598639455782, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17561720, Length=304, Percent_Identity=47.0394736842105, Blast_Score=235, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=48.8262910798122, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17534315, Length=313, Percent_Identity=40.5750798722045, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=314, Percent_Identity=38.8535031847134, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=48.3050847457627, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996324, Length=300, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=38.2445141065831, Blast_Score=199, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=325, Percent_Identity=34.1538461538462, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643623, Length=305, Percent_Identity=40.655737704918, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI20129101, Length=305, Percent_Identity=40.655737704918, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32546; Mature: 32415

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVNSVTELIGDTPAVKLNRIVDEDSADVYVKLEFMNPGSSVKDRIALAMIEAAEKAG
CCCCHHHHHHHHCCCCCEEEHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
RLKPGDTIVEPTSGNTGIGLAMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGA
CCCCCCEEECCCCCCCCCHHHHEECCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECCCC
QGMRGAIQKAEELAREHGYFMPQQFKNEANPEIHRLTTGKEIVEQMGDQLDAFVAGIGTG
CHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
GTITGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGV
CEECHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCE
ITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAVIPSNGERYLS
EEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEC
TPLYQFEN
CCCHHCCC
>Mature Secondary Structure 
ARTVNSVTELIGDTPAVKLNRIVDEDSADVYVKLEFMNPGSSVKDRIALAMIEAAEKAG
CCCHHHHHHHHCCCCCEEEHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
RLKPGDTIVEPTSGNTGIGLAMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGA
CCCCCCEEECCCCCCCCCHHHHEECCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECCCC
QGMRGAIQKAEELAREHGYFMPQQFKNEANPEIHRLTTGKEIVEQMGDQLDAFVAGIGTG
CHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
GTITGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSIYDGV
CEECHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCE
ITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAVIPSNGERYLS
EEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEC
TPLYQFEN
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]