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The map label for this gene is tilS/hprT [H]

Identifier: 138893740

GI number: 138893740

Start: 73126

End: 73671

Strand: Direct

Name: tilS/hprT [H]

Synonym: GTNG_0061

Alternate gene names: 138893740

Gene position: 73126-73671 (Clockwise)

Preceding gene: 138893739

Following gene: 138893741

Centisome position: 2.06

GC content: 43.59

Gene sequence:

>546_bases
ATGGGGATGAAAGACGATATCCAAAAAGTGCTAATCACCGAGGAAGAAATTCAGGCAAAAGTAAAAGAGCTAGGTGCTAT
ATTGACGAAGGAGTATGACGGCCGTTTTCCGCTCGCCGTCGGCGTTCTCAAAGGCGCGATGCCGTTTATGGCCGATTTGT
TGAAACATATTGATACATATTTGGAAATGGATTTTATGGATGTTTCGAGTTATGGGAATGCGACGATTTCATCTGGGGAA
GTAAAAATTATCAAAGATTTAAATACATCCGTTGAAGGTCGCGACATTTTGATCATTGAAGATATTATTGACAGCGGGCT
GACGCTCAGCTATCTGGTCGATTTATTCCGTTACCGGAAGGCGAATTCAATTAAAATCGTCACCCTCCTTGACAAGCCGT
CTGGACGTAAAGCCGATATTCAAGCGGACTACGCCGGATTTACCGTCCCGGATGAGTTCGTCGTTGGTTATGGTCTCGAT
TATGCGGAAAAATATCGCAACCTCCCGTTTATCGGTGTCTTAAAGCCAGAAGTGTACCAAAAATAA

Upstream 100 bases:

>100_bases
TGCCTGGACTGAAAAAATCGGCTTTTGAAGCCGGAGAGCATAGGCAAACTCGCTATATTTTACTGCACTATCAGGCGATG
AACAGCTAGGAGGAAAAAAG

Downstream 100 bases:

>100_bases
GACCGTTGCTGTGCGGCGGCCGAATTGTTTGTATTTAGGCAAATTTCTATGCTAGTATTGAGTATAGTGTGTTTCATATG
GGAGGAGGCAAGAAATGAAC

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MGMKDDIQKVLITEEEIQAKVKELGAILTKEYDGRFPLAVGVLKGAMPFMADLLKHIDTYLEMDFMDVSSYGNATISSGE
VKIIKDLNTSVEGRDILIIEDIIDSGLTLSYLVDLFRYRKANSIKIVTLLDKPSGRKADIQADYAGFTVPDEFVVGYGLD
YAEKYRNLPFIGVLKPEVYQK

Sequences:

>Translated_181_residues
MGMKDDIQKVLITEEEIQAKVKELGAILTKEYDGRFPLAVGVLKGAMPFMADLLKHIDTYLEMDFMDVSSYGNATISSGE
VKIIKDLNTSVEGRDILIIEDIIDSGLTLSYLVDLFRYRKANSIKIVTLLDKPSGRKADIQADYAGFTVPDEFVVGYGLD
YAEKYRNLPFIGVLKPEVYQK
>Mature_180_residues
GMKDDIQKVLITEEEIQAKVKELGAILTKEYDGRFPLAVGVLKGAMPFMADLLKHIDTYLEMDFMDVSSYGNATISSGEV
KIIKDLNTSVEGRDILIIEDIIDSGLTLSYLVDLFRYRKANSIKIVTLLDKPSGRKADIQADYAGFTVPDEFVVGYGLDY
AEKYRNLPFIGVLKPEVYQK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=181, Percent_Identity=36.4640883977901, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI9910262, Length=187, Percent_Identity=35.8288770053476, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI87081689, Length=166, Percent_Identity=48.1927710843374, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17510817, Length=185, Percent_Identity=31.3513513513514, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20285; Mature: 20154

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMKDDIQKVLITEEEIQAKVKELGAILTKEYDGRFPLAVGVLKGAMPFMADLLKHIDTY
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LEMDFMDVSSYGNATISSGEVKIIKDLNTSVEGRDILIIEDIIDSGLTLSYLVDLFRYRK
HHHHHEECCCCCCCEECCCCEEEEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCC
ANSIKIVTLLDKPSGRKADIQADYAGFTVPDEFVVGYGLDYAEKYRNLPFIGVLKPEVYQ
CCCEEEEEEEECCCCCCCCCEECCCCCCCCCHHHEECCCCHHHHHCCCCEEEEECHHHHC
K
C
>Mature Secondary Structure 
GMKDDIQKVLITEEEIQAKVKELGAILTKEYDGRFPLAVGVLKGAMPFMADLLKHIDTY
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LEMDFMDVSSYGNATISSGEVKIIKDLNTSVEGRDILIIEDIIDSGLTLSYLVDLFRYRK
HHHHHEECCCCCCCEECCCCEEEEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCC
ANSIKIVTLLDKPSGRKADIQADYAGFTVPDEFVVGYGLDYAEKYRNLPFIGVLKPEVYQ
CCCEEEEEEEECCCCCCCCCEECCCCCCCCCHHHEECCCCHHHHHCCCCEEEEECHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]