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The map label for this gene is mesJ [H]

Identifier: 138893739

GI number: 138893739

Start: 71720

End: 73114

Strand: Direct

Name: mesJ [H]

Synonym: GTNG_0060

Alternate gene names: 138893739

Gene position: 71720-73114 (Clockwise)

Preceding gene: 138893738

Following gene: 138893740

Centisome position: 2.02

GC content: 49.39

Gene sequence:

>1395_bases
ATGGTTGATAAAGTGCGCGCCTTTATTGACAGGCATAGGTTGCTTACGGAAGGAGCGACGGTCATTGTCGGCGTTTCCGG
AGGACCGGATTCACTTGCTCTTTTGCATCTTTTATCTTCGCTTCGGGATGAGTGGAAGCTTCAGCTCGTGGCGGCTCATG
TCGACCATATGTTTCGCGGGCGGGAATCAGAAGAAGAGATGGAATTTGTGAAACATTTTTGTGCCGAGCACCGTATTTTA
TGTGAAGCGGTGCAAATCGACGTTCCAGCTTTTCAGCGGCGCACAGGTCTCGGCGCTCAGGAGGCGGCTCGAAATTGCCG
GTATCACTTTTTTGCCGAACTGGTGAAAAAGTATCAAGCACAATATGTCGCTTTAGGCCATCATGGTGATGACCAGGTCG
AGACGATTTTAATGCGACTTGTGCGCGGTAGCACAAGCAAAGGGTATGCTGGCATTCCTGTCAAGCGTCCGTTTCATGGC
AGTTATCTCATTCGCCCGTTTTTGGCTGTCAGCCGGGCGGAAATCGAGGACTACTGCAAAAAAGAGGGGCTAACTCCGCG
CCGAGATCCAAGCAATGAGAAAGACGATTATACACGCAATCGATTTCGCCATCATATTGTCCCGCTATTAAAACAGGAAA
ATCCGCGCTTGCATGAACGATTTCAACAATATAGTGAAATGATGGCAGAAGATGAGCAATTTTTAGAGGAATTAGCCACG
GACGCGCTGAATAAAGTAATGGAAAAACAAAATAGCGATGTTGTATTGCGCATTGCCCCGTTTTTGGCGTTGCCGAGACC
GCTGCAGCGTCGGGTGTTACAGCTGTTGCTTCTCCGCCTTTACGGAGGCATTCCGCCGGTGTTGACTTCTGTGCATATCG
GCCATATACTGATGCTTTGTGAGCGCGACCATCCTTCTGGAATGCTCGATTTGCCAAAAGGGTTGAAAGTGATTCGTTCG
TATGACCGCTGTTTGTTTACGTTTGGCCTGCAACATTGCGCTCAAAGCTATTGGCTAGAGCTGCCGGTTCCGGCGCTATT
GCCTCTCCCAAACGGTTACGTAATCATAAGTGAAATTGGGGAACACTATCCAAGAAAGCAGTTAGGAAATGACTCGTTCG
TGGTTGACCCCGCTACGGTTTCTCTTCCGCTTCATGTACGGACGCGCCGCACTGGTGACCGGATGGTGCTGAAGGGGACG
GGAGGAACGAAAAAAATTAAGGAGATTTTTATTGAGGCGAAAATTCCACGGATGGAAAGGGACTGTTGGCCGATTGTAGA
AGATGCCGACGGCCGCATCCTTTGGGTGCCTGGACTGAAAAAATCGGCTTTTGAAGCCGGAGAGCATAGGCAAACTCGCT
ATATTTTACTGCACTATCAGGCGATGAACAGCTAG

Upstream 100 bases:

>100_bases
CTTGGTGCATACGGTTTTTATATATACTGGCAACTAACGATGTAATTTATGGTACAATAGCTATCGATGAGGCTAAAAGA
GAGCAAAGAGGTTTTTTCGA

Downstream 100 bases:

>100_bases
GAGGAAAAAAGATGGGGATGAAAGACGATATCCAAAAAGTGCTAATCACCGAGGAAGAAATTCAGGCAAAAGTAAAAGAG
CTAGGTGCTATATTGACGAA

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MVDKVRAFIDRHRLLTEGATVIVGVSGGPDSLALLHLLSSLRDEWKLQLVAAHVDHMFRGRESEEEMEFVKHFCAEHRIL
CEAVQIDVPAFQRRTGLGAQEAARNCRYHFFAELVKKYQAQYVALGHHGDDQVETILMRLVRGSTSKGYAGIPVKRPFHG
SYLIRPFLAVSRAEIEDYCKKEGLTPRRDPSNEKDDYTRNRFRHHIVPLLKQENPRLHERFQQYSEMMAEDEQFLEELAT
DALNKVMEKQNSDVVLRIAPFLALPRPLQRRVLQLLLLRLYGGIPPVLTSVHIGHILMLCERDHPSGMLDLPKGLKVIRS
YDRCLFTFGLQHCAQSYWLELPVPALLPLPNGYVIISEIGEHYPRKQLGNDSFVVDPATVSLPLHVRTRRTGDRMVLKGT
GGTKKIKEIFIEAKIPRMERDCWPIVEDADGRILWVPGLKKSAFEAGEHRQTRYILLHYQAMNS

Sequences:

>Translated_464_residues
MVDKVRAFIDRHRLLTEGATVIVGVSGGPDSLALLHLLSSLRDEWKLQLVAAHVDHMFRGRESEEEMEFVKHFCAEHRIL
CEAVQIDVPAFQRRTGLGAQEAARNCRYHFFAELVKKYQAQYVALGHHGDDQVETILMRLVRGSTSKGYAGIPVKRPFHG
SYLIRPFLAVSRAEIEDYCKKEGLTPRRDPSNEKDDYTRNRFRHHIVPLLKQENPRLHERFQQYSEMMAEDEQFLEELAT
DALNKVMEKQNSDVVLRIAPFLALPRPLQRRVLQLLLLRLYGGIPPVLTSVHIGHILMLCERDHPSGMLDLPKGLKVIRS
YDRCLFTFGLQHCAQSYWLELPVPALLPLPNGYVIISEIGEHYPRKQLGNDSFVVDPATVSLPLHVRTRRTGDRMVLKGT
GGTKKIKEIFIEAKIPRMERDCWPIVEDADGRILWVPGLKKSAFEAGEHRQTRYILLHYQAMNS
>Mature_464_residues
MVDKVRAFIDRHRLLTEGATVIVGVSGGPDSLALLHLLSSLRDEWKLQLVAAHVDHMFRGRESEEEMEFVKHFCAEHRIL
CEAVQIDVPAFQRRTGLGAQEAARNCRYHFFAELVKKYQAQYVALGHHGDDQVETILMRLVRGSTSKGYAGIPVKRPFHG
SYLIRPFLAVSRAEIEDYCKKEGLTPRRDPSNEKDDYTRNRFRHHIVPLLKQENPRLHERFQQYSEMMAEDEQFLEELAT
DALNKVMEKQNSDVVLRIAPFLALPRPLQRRVLQLLLLRLYGGIPPVLTSVHIGHILMLCERDHPSGMLDLPKGLKVIRS
YDRCLFTFGLQHCAQSYWLELPVPALLPLPNGYVIISEIGEHYPRKQLGNDSFVVDPATVSLPLHVRTRRTGDRMVLKGT
GGTKKIKEIFIEAKIPRMERDCWPIVEDADGRILWVPGLKKSAFEAGEHRQTRYILLHYQAMNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=422, Percent_Identity=28.6729857819905, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53244; Mature: 53244

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKVRAFIDRHRLLTEGATVIVGVSGGPDSLALLHLLSSLRDEWKLQLVAAHVDHMFRG
CCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RESEEEMEFVKHFCAEHRILCEAVQIDVPAFQRRTGLGAQEAARNCRYHFFAELVKKYQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHH
QYVALGHHGDDQVETILMRLVRGSTSKGYAGIPVKRPFHGSYLIRPFLAVSRAEIEDYCK
HEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KEGLTPRRDPSNEKDDYTRNRFRHHIVPLLKQENPRLHERFQQYSEMMAEDEQFLEELAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DALNKVMEKQNSDVVLRIAPFLALPRPLQRRVLQLLLLRLYGGIPPVLTSVHIGHILMLC
HHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEE
ERDHPSGMLDLPKGLKVIRSYDRCLFTFGLQHCAQSYWLELPVPALLPLPNGYVIISEIG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEHHH
EHYPRKQLGNDSFVVDPATVSLPLHVRTRRTGDRMVLKGTGGTKKIKEIFIEAKIPRMER
CCCCHHHCCCCCEEECCCEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
DCWPIVEDADGRILWVPGLKKSAFEAGEHRQTRYILLHYQAMNS
CCCCCEECCCCCEEECCCCCHHHHHCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MVDKVRAFIDRHRLLTEGATVIVGVSGGPDSLALLHLLSSLRDEWKLQLVAAHVDHMFRG
CCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RESEEEMEFVKHFCAEHRILCEAVQIDVPAFQRRTGLGAQEAARNCRYHFFAELVKKYQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHH
QYVALGHHGDDQVETILMRLVRGSTSKGYAGIPVKRPFHGSYLIRPFLAVSRAEIEDYCK
HEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KEGLTPRRDPSNEKDDYTRNRFRHHIVPLLKQENPRLHERFQQYSEMMAEDEQFLEELAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DALNKVMEKQNSDVVLRIAPFLALPRPLQRRVLQLLLLRLYGGIPPVLTSVHIGHILMLC
HHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEE
ERDHPSGMLDLPKGLKVIRSYDRCLFTFGLQHCAQSYWLELPVPALLPLPNGYVIISEIG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEHHH
EHYPRKQLGNDSFVVDPATVSLPLHVRTRRTGDRMVLKGTGGTKKIKEIFIEAKIPRMER
CCCCHHHCCCCCEEECCCEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
DCWPIVEDADGRILWVPGLKKSAFEAGEHRQTRYILLHYQAMNS
CCCCCEECCCCCEEECCCCCHHHHHCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA