Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 138893709

GI number: 138893709

Start: 42085

End: 44037

Strand: Direct

Name: metG [H]

Synonym: GTNG_0030

Alternate gene names: 138893709

Gene position: 42085-44037 (Clockwise)

Preceding gene: 138893707

Following gene: 138893710

Centisome position: 1.19

GC content: 49.0

Gene sequence:

>1953_bases
ATGGAGAAAAAGACGTTTTATTTGACGACGCCGATTTATTATCCGAGCGACAAATTGCACATCGGCCATGCTTATACAAC
AGTGGCGGGGGATACGCTAGCGCGCTATAAACGGATGCGCGGTTACGATGTTATGTATTTGACGGGAACCGATGAGCACG
GGCAAAAAATTCAACGCAAGGCGGAGGAAAAAGGAGTAACGCCGCAGCAATATGTCGATGAGATCGTCGCTGGCATTCAG
GAGCTATGGAAAAAGCTCGACATTTCTTATGACGATTTCATCCGTACAACGCAGGAGCGGCATAAAAAAGTAGTCGAAAA
GATTTTCGCGCGTCTTGTCGAACAAGGGGATATTTATTTAGGTGAATATGAAGGATGGTATTGCACGCCATGCGAATCGT
TTTACACTGAGCGGCAGCTTGTCGACGGCAACTGCCCGGACTGTGGTCGTCCGGTTGAAAAAGTGAAAGAGCAGTCGTAC
TTTTTCCGAATGAGCAAATACGTCGACCGTTTGCTTCAATATTATGAGGAAAATCCAGATTTCATCCAGCCGGAATCGCG
GAAAAACGAAATGATTAACAATTTTATTAAGCCGGGGCTTGAAGATTTAGCTGTGTCGCGGACGACGTTTGACTGGGGCA
TTAAAGTGCCGGGCGATCCGAAACATGTCATTTACGTCTGGATTGACGCGCTTGCCAACTATATTACAGCGCTCGGTTAC
GGCACGGACAATGATGAAAAGTTCCGCAAATATTGGCCGGCCGATGTCCATTTAGTCGGCAAGGAAATCATCCGCTTTCA
TACGATTTATTGGCCGATTATGCTCATGGCGCTTGACTTGCCGCTGCCGAAAAAAGTATTCGGTCATGGCTGGCTGCTCA
TGAAAGACGGGAAAATGTCGAAATCGAAAGGCAATGTCGTTGACCCGGTGACGTTGATCGATCGATACGGACTCGATGCG
CTTCGTTATTATTTACTCAGGGAAGTGCCGTTCGGTTCTGACGGCGTATTCACGCCGGAAGGATTTATTGAGCGCATCAA
CTACGATTTAGCCAATGACCTAGGCAATTTATTGAATCGTACAGTAGCGATGATTAAGAAATATTTTGATGGGGTGATTC
CGCCGTACCGCGGTCCGAAAACGCCGTTTGACGAAGAGCTGGTACAAACGGCGCGTGAGGTGGTCCGTCAGTATGAGGAA
GCGATGGAACGGATGGAGTTTTCCGTTGCCCTTGCTTCGGTTTGGCAACTGATTGGCCGGACGAACAAATACATTGATGA
GACGCAGCCATGGGTATTGGCCAAAGATGAAAGCAAACGGGAAGAGCTTGCTTCTGTCATGACCCACCTAGCCGAGTCGC
TCCGCCATACGGCAGTGCTGTTGCAGCCGTTTTTGACACGCACGCCAGAGCGCATTTTTGCCCAGCTCGGCATTGCCGAC
CGTTCATTAAAAGAGTGGGATAGCTTGTACGAGTTCGGGCTCATTCCGGAAGGAACAAACGTGCAAAAAGGAGAACCACT
GTTCCCGCGCCTTGATATTGAAGCGGAAGTCGAGTACATTAAGGCGCATATGCAAGGCGGCAAGCCGGCGGTGGAACCCG
TTAAAGAGGAGAAGCAAGCGGCTGAGACGGCCGAAATCTCAATTGATGAGTTTGCCAAAGTTGACTTGCGCGTTGCTGAA
GTCGTGCATGCTGAACGGATGAAAAACGCCAATAAGCTGTTGAACCTCCAACTTGATCTTGGCGGCGAGAAACGGCAAGT
CATCTCTGGTATCGCTGAATTTTACAAACCAGAGGAACTCATCGGCAAAAAGGTCATTTGCGTCGCCAATTTAAAACCGG
CCAAACTGCGCGGTGAGTGGTCGGAAGGAATGATTTTGGCCGGCGGTAACGGCGGAGAGTTTTCACTGGCGACCGTCGAT
CAACATGTGCCAAACGGAACAAAAATTAAATAA

Upstream 100 bases:

>100_bases
TTTTTGTGGAATGAAAAAATTATGTAAAATAGAAGTAACACCGCTCCCGTCGCCAGATCGGGGGTTTTTGTGCTTAACAG
TACGAGGGAGGGCCTAACTG

Downstream 100 bases:

>100_bases
CATAATCGCTTAATCGGCGAGGGGGTGTCGACCTTGACACCTCCTTGTCGTTATTTTGTAATGTAAATGTTAATGAACTG
TGATATACGTAATCAATTTC

Product: methionine-tRNA ligase/methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MEKKTFYLTTPIYYPSDKLHIGHAYTTVAGDTLARYKRMRGYDVMYLTGTDEHGQKIQRKAEEKGVTPQQYVDEIVAGIQ
ELWKKLDISYDDFIRTTQERHKKVVEKIFARLVEQGDIYLGEYEGWYCTPCESFYTERQLVDGNCPDCGRPVEKVKEQSY
FFRMSKYVDRLLQYYEENPDFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGDPKHVIYVWIDALANYITALGY
GTDNDEKFRKYWPADVHLVGKEIIRFHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVDPVTLIDRYGLDA
LRYYLLREVPFGSDGVFTPEGFIERINYDLANDLGNLLNRTVAMIKKYFDGVIPPYRGPKTPFDEELVQTAREVVRQYEE
AMERMEFSVALASVWQLIGRTNKYIDETQPWVLAKDESKREELASVMTHLAESLRHTAVLLQPFLTRTPERIFAQLGIAD
RSLKEWDSLYEFGLIPEGTNVQKGEPLFPRLDIEAEVEYIKAHMQGGKPAVEPVKEEKQAAETAEISIDEFAKVDLRVAE
VVHAERMKNANKLLNLQLDLGGEKRQVISGIAEFYKPEELIGKKVICVANLKPAKLRGEWSEGMILAGGNGGEFSLATVD
QHVPNGTKIK

Sequences:

>Translated_650_residues
MEKKTFYLTTPIYYPSDKLHIGHAYTTVAGDTLARYKRMRGYDVMYLTGTDEHGQKIQRKAEEKGVTPQQYVDEIVAGIQ
ELWKKLDISYDDFIRTTQERHKKVVEKIFARLVEQGDIYLGEYEGWYCTPCESFYTERQLVDGNCPDCGRPVEKVKEQSY
FFRMSKYVDRLLQYYEENPDFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGDPKHVIYVWIDALANYITALGY
GTDNDEKFRKYWPADVHLVGKEIIRFHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVDPVTLIDRYGLDA
LRYYLLREVPFGSDGVFTPEGFIERINYDLANDLGNLLNRTVAMIKKYFDGVIPPYRGPKTPFDEELVQTAREVVRQYEE
AMERMEFSVALASVWQLIGRTNKYIDETQPWVLAKDESKREELASVMTHLAESLRHTAVLLQPFLTRTPERIFAQLGIAD
RSLKEWDSLYEFGLIPEGTNVQKGEPLFPRLDIEAEVEYIKAHMQGGKPAVEPVKEEKQAAETAEISIDEFAKVDLRVAE
VVHAERMKNANKLLNLQLDLGGEKRQVISGIAEFYKPEELIGKKVICVANLKPAKLRGEWSEGMILAGGNGGEFSLATVD
QHVPNGTKIK
>Mature_650_residues
MEKKTFYLTTPIYYPSDKLHIGHAYTTVAGDTLARYKRMRGYDVMYLTGTDEHGQKIQRKAEEKGVTPQQYVDEIVAGIQ
ELWKKLDISYDDFIRTTQERHKKVVEKIFARLVEQGDIYLGEYEGWYCTPCESFYTERQLVDGNCPDCGRPVEKVKEQSY
FFRMSKYVDRLLQYYEENPDFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGDPKHVIYVWIDALANYITALGY
GTDNDEKFRKYWPADVHLVGKEIIRFHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVDPVTLIDRYGLDA
LRYYLLREVPFGSDGVFTPEGFIERINYDLANDLGNLLNRTVAMIKKYFDGVIPPYRGPKTPFDEELVQTAREVVRQYEE
AMERMEFSVALASVWQLIGRTNKYIDETQPWVLAKDESKREELASVMTHLAESLRHTAVLLQPFLTRTPERIFAQLGIAD
RSLKEWDSLYEFGLIPEGTNVQKGEPLFPRLDIEAEVEYIKAHMQGGKPAVEPVKEEKQAAETAEISIDEFAKVDLRVAE
VVHAERMKNANKLLNLQLDLGGEKRQVISGIAEFYKPEELIGKKVICVANLKPAKLRGEWSEGMILAGGNGGEFSLATVD
QHVPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=551, Percent_Identity=34.4827586206897, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI14043022, Length=652, Percent_Identity=26.840490797546, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI7661872, Length=188, Percent_Identity=27.6595744680851, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=30.8588064046579, Blast_Score=320, Evalue=3e-88,
Organism=Escherichia coli, GI1786861, Length=173, Percent_Identity=27.1676300578035, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=35.8490566037736, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI212645096, Length=497, Percent_Identity=32.1931589537223, Blast_Score=272, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI71992781, Length=385, Percent_Identity=31.1688311688312, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17540794, Length=592, Percent_Identity=25.8445945945946, Blast_Score=185, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI71997510, Length=168, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71997517, Length=168, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321610, Length=516, Percent_Identity=31.3953488372093, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321704, Length=553, Percent_Identity=24.2314647377938, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323414, Length=215, Percent_Identity=25.1162790697674, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358483, Length=528, Percent_Identity=33.7121212121212, Blast_Score=296, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24655311, Length=641, Percent_Identity=26.2090483619345, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21355409, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74640; Mature: 74640

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKTFYLTTPIYYPSDKLHIGHAYTTVAGDTLARYKRMRGYDVMYLTGTDEHGQKIQRK
CCCCEEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AEEKGVTPQQYVDEIVAGIQELWKKLDISYDDFIRTTQERHKKVVEKIFARLVEQGDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GEYEGWYCTPCESFYTERQLVDGNCPDCGRPVEKVKEQSYFFRMSKYVDRLLQYYEENPD
ECCCCEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGDPKHVIYVWIDALANYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHHCEEEECCEECCCCCCEEEEEEHHHHHHHHHHHCC
GTDNDEKFRKYWPADVHLVGKEIIRFHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMS
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFIERINYDLANDLGNLLNR
CCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHH
TVAMIKKYFDGVIPPYRGPKTPFDEELVQTAREVVRQYEEAMERMEFSVALASVWQLIGR
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNKYIDETQPWVLAKDESKREELASVMTHLAESLRHTAVLLQPFLTRTPERIFAQLGIAD
CHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCH
RSLKEWDSLYEFGLIPEGTNVQKGEPLFPRLDIEAEVEYIKAHMQGGKPAVEPVKEEKQA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AETAEISIDEFAKVDLRVAEVVHAERMKNANKLLNLQLDLGGEKRQVISGIAEFYKPEEL
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHCCCHHH
IGKKVICVANLKPAKLRGEWSEGMILAGGNGGEFSLATVDQHVPNGTKIK
HCCEEEEEECCCCHHCCCCCCCCEEEECCCCCCEEEEEHHHHCCCCCCCC
>Mature Secondary Structure
MEKKTFYLTTPIYYPSDKLHIGHAYTTVAGDTLARYKRMRGYDVMYLTGTDEHGQKIQRK
CCCCEEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AEEKGVTPQQYVDEIVAGIQELWKKLDISYDDFIRTTQERHKKVVEKIFARLVEQGDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GEYEGWYCTPCESFYTERQLVDGNCPDCGRPVEKVKEQSYFFRMSKYVDRLLQYYEENPD
ECCCCEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGDPKHVIYVWIDALANYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHHCEEEECCEECCCCCCEEEEEEHHHHHHHHHHHCC
GTDNDEKFRKYWPADVHLVGKEIIRFHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMS
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFIERINYDLANDLGNLLNR
CCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHH
TVAMIKKYFDGVIPPYRGPKTPFDEELVQTAREVVRQYEEAMERMEFSVALASVWQLIGR
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNKYIDETQPWVLAKDESKREELASVMTHLAESLRHTAVLLQPFLTRTPERIFAQLGIAD
CHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCH
RSLKEWDSLYEFGLIPEGTNVQKGEPLFPRLDIEAEVEYIKAHMQGGKPAVEPVKEEKQA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AETAEISIDEFAKVDLRVAEVVHAERMKNANKLLNLQLDLGGEKRQVISGIAEFYKPEEL
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHCCCHHH
IGKKVICVANLKPAKLRGEWSEGMILAGGNGGEFSLATVDQHVPNGTKIK
HCCEEEEEECCCCHHCCCCCCCCEEEECCCCCCEEEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1852609 [H]