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The map label for this gene is hsdR

Identifier: 13508086

GI number: 13508086

Start: 413985

End: 415115

Strand: Direct

Name: hsdR

Synonym: MPN347

Alternate gene names: NA

Gene position: 413985-415115 (Clockwise)

Preceding gene: 13508085

Following gene: 13508087

Centisome position: 50.71

GC content: 30.33

Gene sequence:

>1131_bases
ATGTTCTTAACTGGTTTTGATGCACCAACCCTTAATACACTTTGAGTAGATAAGAATTTAAGCGAACACACTTTAATCCA
AGCTTTTTCAAGAACTAATAGATGTTTTGGAGAAACTAAAAAATTCGGAAACATTTTTAGCTTTAGAGATATAAGAGAAA
ACTTCGATCAAGCTCTAAAAATGTACGCAGACAAGCGTGGTCTTAAGGATATAAAATCCAACATGGTTATGCGAGAATTT
GAGGAAGTTTTTGAAAAATTCAAAGATTGAACCGAGAAAATAAAACAAAAATACCCAAATCCAGATGAAATTCTTAATTT
AGAAAAAGAAGAAAAGTGAGATTTTTCTGATCTTTTTGGTGAATTTCTTGGAGATTACAGAACTGTAAAAAGCTATCATG
AATTCGACAAAGACAACCCTTATCTTCCAAGTGATGAATTCAATAAATATTTTGCTGCGTTTTCTCAAATTCAAGAAGAG
ATAAGGAAAGAAAAGGCAGAGAAAAAAGCGCTAGAAGTACAAGAAGAAGATTTATCAAATGAAGTACGAAAAGAACCGCA
AATCTTTTCTTGAGAATATGGTGAGGGTATTCAAGCCAATTTAGATTACATTTTTCATCTAATTAAGGATCTCTATAATT
GTGATAATGAGCAAGATAGACAAAAAGTATTAGACAAAATTAAGACTGCAATTAGAACAAATCCAGAGATTCAAGTGAAT
GAAGATATTCTTAATGAATTTATAGAGGACTTAGGGAAGTCGTTCGAGAAAGAAAAACTTGAAGAAGTTTGAAAAACAGA
AGGTAAATTACGTGAGTGTTTAAAGGATTTCTGTAAAAAGAAGGAAGAAAGCAATAAGAAAAAAATTATTAATGACTTTT
TAGTTAAAGGAGTTTTCCTTGTTGAATGAGTAGCTGATAAACAACTAAATACAGCTAAAAAGAAAGGTGAATACAGTCCT
TCTTGATCTGATACAGAAAAATTAATTTGAAAAGAAAAATCAACCAATCTTCCAACTATGACAGAAAAAGATGAAGATGG
CAATTTCAAGTGAAGAAAATTAATTACAGATATCTCGGAAGCTTTTAAAAATCACTTCGAAAGATTTATCCGCTTTGTTG
AAATTAGATAG

Upstream 100 bases:

>100_bases
AAATGTTTCTGATAGATTAAAGCCAAAAAAGACAAAAAATTTAGATGGTGAAAATAAAAACACAAATGGCTGAGAAATCG
ACATTTTAATTGTTGTTAAC

Downstream 100 bases:

>100_bases
TTTTAAATTATTATCTATAAATCAGGTTTTCAACCATAGATTACTATGGAAAAGAAAAATAAAAAAAATACCGCAACCGT
TCCTACCAAAGAACAATTGG

Product: type I restriction enzyme

Products: NA

Alternate protein names: MpnORFDAP [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFLTGFDAPTLNTLWVDKNLSEHTLIQAFSRTNRCFGETKKFGNIFSFRDIRENFDQALKMYADKRGLKDIKSNMVMREF
EEVFEKFKDWTEKIKQKYPNPDEILNLEKEEKWDFSDLFGEFLGDYRTVKSYHEFDKDNPYLPSDEFNKYFAAFSQIQEE
IRKEKAEKKALEVQEEDLSNEVRKEPQIFSWEYGEGIQANLDYIFHLIKDLYNCDNEQDRQKVLDKIKTAIRTNPEIQVN
EDILNEFIEDLGKSFEKEKLEEVWKTEGKLRECLKDFCKKKEESNKKKIINDFLVKGVFLVEWVADKQLNTAKKKGEYSP
SWSDTEKLIWKEKSTNLPTMTEKDEDGNFKWRKLITDISEAFKNHFERFIRFVEIR

Sequences:

>Translated_376_residues
MFLTGFDAPTLNTL*VDKNLSEHTLIQAFSRTNRCFGETKKFGNIFSFRDIRENFDQALKMYADKRGLKDIKSNMVMREF
EEVFEKFKD*TEKIKQKYPNPDEILNLEKEEK*DFSDLFGEFLGDYRTVKSYHEFDKDNPYLPSDEFNKYFAAFSQIQEE
IRKEKAEKKALEVQEEDLSNEVRKEPQIFS*EYGEGIQANLDYIFHLIKDLYNCDNEQDRQKVLDKIKTAIRTNPEIQVN
EDILNEFIEDLGKSFEKEKLEEV*KTEGKLRECLKDFCKKKEESNKKKIINDFLVKGVFLVE*VADKQLNTAKKKGEYSP
S*SDTEKLI*KEKSTNLPTMTEKDEDGNFK*RKLITDISEAFKNHFERFIRFVEIR
>Mature_376_residues
MFLTGFDAPTLNTL*VDKNLSEHTLIQAFSRTNRCFGETKKFGNIFSFRDIRENFDQALKMYADKRGLKDIKSNMVMREF
EEVFEKFKD*TEKIKQKYPNPDEILNLEKEEK*DFSDLFGEFLGDYRTVKSYHEFDKDNPYLPSDEFNKYFAAFSQIQEE
IRKEKAEKKALEVQEEDLSNEVRKEPQIFS*EYGEGIQANLDYIFHLIKDLYNCDNEQDRQKVLDKIKTAIRTNPEIQVN
EDILNEFIEDLGKSFEKEKLEEV*KTEGKLRECLKDFCKKKEESNKKKIINDFLVKGVFLVE*VADKQLNTAKKKGEYSP
S*SDTEKLI*KEKSTNLPTMTEKDEDGNFK*RKLITDISEAFKNHFERFIRFVEIR

Specific function: Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG0610

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022625 [H]

Pfam domain/function: PF12008 EcoR124_C [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 43249; Mature: 43249

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLTGFDAPTLNTLVDKNLSEHTLIQAFSRTNRCFGETKKFGNIFSFRDIRENFDQALKM
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
YADKRGLKDIKSNMVMREFEEVFEKFKDTEKIKQKYPNPDEILNLEKEEKDFSDLFGEFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
GDYRTVKSYHEFDKDNPYLPSDEFNKYFAAFSQIQEEIRKEKAEKKALEVQEEDLSNEVR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEPQIFSEYGEGIQANLDYIFHLIKDLYNCDNEQDRQKVLDKIKTAIRTNPEIQVNEDIL
HCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEECHHHH
NEFIEDLGKSFEKEKLEEVKTEGKLRECLKDFCKKKEESNKKKIINDFLVKGVFLVEVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLNTAKKKGEYSPSSDTEKLIKEKSTNLPTMTEKDEDGNFKRKLITDISEAFKNHFERF
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IRFVEIR
HHHHHCC
>Mature Secondary Structure
MFLTGFDAPTLNTLVDKNLSEHTLIQAFSRTNRCFGETKKFGNIFSFRDIRENFDQALKM
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
YADKRGLKDIKSNMVMREFEEVFEKFKDTEKIKQKYPNPDEILNLEKEEKDFSDLFGEFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
GDYRTVKSYHEFDKDNPYLPSDEFNKYFAAFSQIQEEIRKEKAEKKALEVQEEDLSNEVR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEPQIFSEYGEGIQANLDYIFHLIKDLYNCDNEQDRQKVLDKIKTAIRTNPEIQVNEDIL
HCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEECHHHH
NEFIEDLGKSFEKEKLEEVKTEGKLRECLKDFCKKKEESNKKKIINDFLVKGVFLVEVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLNTAKKKGEYSPSSDTEKLIKEKSTNLPTMTEKDEDGNFKRKLITDISEAFKNHFERF
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IRFVEIR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]