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The map label for this gene is pcrA [C]

Identifier: 13508079

GI number: 13508079

Start: 403921

End: 405510

Strand: Direct

Name: pcrA [C]

Synonym: MPN340

Alternate gene names: 13508079

Gene position: 403921-405510 (Clockwise)

Preceding gene: 13508078

Following gene: 13508080

Centisome position: 49.48

GC content: 37.42

Gene sequence:

>1590_bases
ATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCAC
GGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCT
TTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTG
CACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGCGATTC
TAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATT
TAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATC
TCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGA
TTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGC
ATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCT
AATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAA
AAATGATTTTCAAGCAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCATCCAAGCGGTGTTAGCAGTAGCGAATG
ATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCG
GTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGG
TGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATG
GTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTAT
GGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTT
TTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGC
CGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACA
CAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGAAT
TATTTTAGGGGCGATTCTTTTGATAATTGTGATTATTACCGCTGTGTTAAAACTTTTTGTTGAAAATTAA

Upstream 100 bases:

>100_bases
AGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAG
AACAATTAGGTGATTAAGTT

Downstream 100 bases:

>100_bases
CATTTTATCCCTTTTTTATAACAGATTACACTAATTAAAAAAACAATCTTAAACCTAGTACTATGGCATTTAACATTAGC
ACTCAACTCAATAAGGAACA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDL
HIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTI
SNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHP
NLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVP
VHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVY
GLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKT
QPKPVNLYTETENAENLQKATDSKKWIILGAILLIIVIITAVLKLFVEN

Sequences:

>Translated_529_residues
MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDL
HIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTI
SNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHP
NLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVP
VHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVY
GLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKT
QPKPVNLYTETENAENLQKATDSKK*IILGAILLIIVIITAVLKLFVEN
>Mature_529_residues
MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDL
HIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTI
SNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHP
NLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVP
VHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVY
GLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKT
QPKPVNLYTETENAENLQKATDSKK*IILGAILLIIVIITAVLKLFVEN

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=386, Percent_Identity=30.5699481865285, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI48994965, Length=373, Percent_Identity=32.171581769437, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787196, Length=317, Percent_Identity=26.4984227129338, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=410, Percent_Identity=27.3170731707317, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324477, Length=395, Percent_Identity=23.0379746835443, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 60326; Mature: 60326

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKA
CCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHEEEEEECHHH
ASEMRQRIIKLIPQKSLQDLHIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQL
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCHHH
QTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTISNGQFRKRAATALRAYQNYL
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHP
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCC
NLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIA
CEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKERKALVLLHSSIDSKAVPVHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKN
HHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCC
KHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVYGLVERAFPSLHWDGSDKHKL
CCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCHHHHH
LEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKT
HHHHHHHHHHHHHHHHEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPKPVNLYTETENAENLQKATDSKKIILGAILLIIVIITAVLKLFVEN
CCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKA
CCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHEEEEEECHHH
ASEMRQRIIKLIPQKSLQDLHIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQL
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCHHH
QTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTISNGQFRKRAATALRAYQNYL
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHP
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCC
NLFLVGDSNQMIYGWRGAVVEIFELLKNDFQAVKEFYTTQNYRSIQAVLAVANDVLTAIA
CEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKERKALVLLHSSIDSKAVPVHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKN
HHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCC
KHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVYGLVERAFPSLHWDGSDKHKL
CCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCHHHHH
LEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKT
HHHHHHHHHHHHHHHHEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPKPVNLYTETENAENLQKATDSKKIILGAILLIIVIITAVLKLFVEN
CCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]