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The map label for this gene is pyk [H]

Identifier: 13508042

GI number: 13508042

Start: 356677

End: 358203

Strand: Direct

Name: pyk [H]

Synonym: MPN303

Alternate gene names: 13508042

Gene position: 356677-358203 (Clockwise)

Preceding gene: 13508041

Following gene: 13508043

Centisome position: 43.69

GC content: 42.83

Gene sequence:

>1527_bases
ATGATTCACCACCTAAAACGCACTAAGATTATTGCCACCTGTGGTCCGGCTTTAACTAAAAAGCTATGAACGCTAGCAAT
GTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGG
TAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAG
CTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAA
GATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATG
CTAGTGGCACTTACAACATGGTAAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTC
GTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTT
AAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACC
AAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAAT
GCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGA
TGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGACAACGGTACATGATAA
AAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACT
CGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGG
GGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACT
TGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGAAAGCAGGTGGTGTTACCGTTAGCACAAAAAACA
GCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTA
TGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTG
ATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTT
GCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAG
CTTATAA

Upstream 100 bases:

>100_bases
ATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAAT
AAAAATATTATTTAACTGAT

Downstream 100 bases:

>100_bases
GCGCTAAAAATTAATTAGAGTTTGTAAAATATTCTACACGGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACC
TCCATATCGTCAGTTCGAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MIHHLKRTKIIATCGPALTKKLWTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKK
LNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLV
VKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKN
AKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYWQRYMIKACRFFNKRVITATQMLDSLEKNIQPT
RAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEFWKQVVLPLAQKT
APKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANV
AIKHYQQHGEISQLKQCLGVFHNKIISL

Sequences:

>Translated_508_residues
MIHHLKRTKIIATCGPALTKKL*TLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKK
LNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLV
VKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKN
AKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPY*QRYMIKACRFFNKRVITATQMLDSLEKNIQPT
RAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEF*KQVVLPLAQKT
APKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANV
AIKHYQQHGEISQLKQCLGVFHNKIISL
>Mature_508_residues
MIHHLKRTKIIATCGPALTKKL*TLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKK
LNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLV
VKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKN
AKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPY*QRYMIKACRFFNKRVITATQMLDSLEKNIQPT
RAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEF*KQVVLPLAQKT
APKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANV
AIKHYQQHGEISQLKQCLGVFHNKIISL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=475, Percent_Identity=33.8947368421053, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI32967597, Length=475, Percent_Identity=33.8947368421053, Blast_Score=236, Evalue=5e-62,
Organism=Homo sapiens, GI33286418, Length=467, Percent_Identity=34.475374732334, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI33286422, Length=465, Percent_Identity=34.6236559139785, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI33286420, Length=465, Percent_Identity=34.6236559139785, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI310128732, Length=312, Percent_Identity=35.8974358974359, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI310128730, Length=312, Percent_Identity=35.8974358974359, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI310128736, Length=260, Percent_Identity=31.9230769230769, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128734, Length=260, Percent_Identity=31.9230769230769, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128738, Length=220, Percent_Identity=34.5454545454545, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787965, Length=458, Percent_Identity=35.1528384279476, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1788160, Length=370, Percent_Identity=34.5945945945946, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17544584, Length=379, Percent_Identity=37.7308707124011, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=392, Percent_Identity=36.2244897959184, Blast_Score=211, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17506829, Length=392, Percent_Identity=36.2244897959184, Blast_Score=211, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984406, Length=392, Percent_Identity=36.2244897959184, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17506831, Length=392, Percent_Identity=36.2244897959184, Blast_Score=210, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324923, Length=465, Percent_Identity=34.6236559139785, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319279, Length=491, Percent_Identity=32.9938900203666, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28571814, Length=381, Percent_Identity=38.0577427821522, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24648964, Length=381, Percent_Identity=38.0577427821522, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24648966, Length=431, Percent_Identity=33.8747099767981, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24581235, Length=340, Percent_Identity=30, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=33.457249070632, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 56656; Mature: 56656

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHHLKRTKIIATCGPALTKKLTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSH
CCCCCCCCEEEEECCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
GNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTK
CCCCCHHEEHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCEEEECCHH
EVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTI
HHHCCCCEEEECCCCCCCHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEECCCCEE
FTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
KHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYQRYMIKACRFFNKR
CEEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCEECCEEECCHHHHHHHHHHHHCCH
VITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDK
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCC
QSETFFDYQYNLNYYMANSKARHSEFKQVVLPLAQKTAPKRKLINSDFKYDFVVHATNNL
CCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEECCCC
NEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIK
CEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHH
HYQQHGEISQLKQCLGVFHNKIISL
HHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIHHLKRTKIIATCGPALTKKLTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSH
CCCCCCCCEEEEECCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECC
GNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTK
CCCCCHHEEHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCEEEECCCEEEECCHH
EVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTI
HHHCCCCEEEECCCCCCCHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEECCCCEE
FTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNA
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
KHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYQRYMIKACRFFNKR
CEEEEEEEECCCCCCCCCCCEEEECCCEEEEECCCCEECCEEECCHHHHHHHHHHHHCCH
VITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDK
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCC
QSETFFDYQYNLNYYMANSKARHSEFKQVVLPLAQKTAPKRKLINSDFKYDFVVHATNNL
CCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEECCCC
NEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIK
CEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHH
HYQQHGEISQLKQCLGVFHNKIISL
HHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]