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The map label for this gene is pfkA

Identifier: 13508041

GI number: 13508041

Start: 355685

End: 356671

Strand: Direct

Name: pfkA

Synonym: MPN302

Alternate gene names: 13508041

Gene position: 355685-356671 (Clockwise)

Preceding gene: 13508040

Following gene: 13508042

Centisome position: 43.57

GC content: 42.65

Gene sequence:

>987_bases
ATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTA
CCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGT
TAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAA
TTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGG
TGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACG
ACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCAC
ACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTT
AGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCT
TGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATC
AAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAG
CCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTG
GTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATT
GCAGTCATTAATAAAAATATTATTTAA

Upstream 100 bases:

>100_bases
CTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTA
AGGTTATATAATTAGCGCAA

Downstream 100 bases:

>100_bases
CTGATATGATTCACCACCTAAAACGCACTAAGATTATTGCCACCTGTGGTCCGGCTTTAACTAAAAAGCTATGAACGCTA
GCAATGTTAGATGACCCAGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVE
FKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYH
TTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAI
KQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALI
AVINKNII

Sequences:

>Translated_328_residues
MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVE
FKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYH
TTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAI
KQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALI
AVINKNII
>Mature_327_residues
SPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVEF
KELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHT
TKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAIK
QHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALIA
VINKNII

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453768, Length=302, Percent_Identity=35.7615894039735, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI266453748, Length=302, Percent_Identity=35.7615894039735, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4505749, Length=302, Percent_Identity=35.7615894039735, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI266453619, Length=302, Percent_Identity=35.7615894039735, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=32.475884244373, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI11321601, Length=306, Percent_Identity=32.6797385620915, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1790350, Length=307, Percent_Identity=39.413680781759, Blast_Score=228, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI25147584, Length=297, Percent_Identity=34.3434343434343, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558788, Length=292, Percent_Identity=33.2191780821918, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI72003601, Length=311, Percent_Identity=25.7234726688103, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321679, Length=293, Percent_Identity=35.4948805460751, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323861, Length=315, Percent_Identity=30.7936507936508, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI28573326, Length=351, Percent_Identity=34.7578347578348, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17647809, Length=357, Percent_Identity=33.8935574229692, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24652337, Length=351, Percent_Identity=34.1880341880342, Blast_Score=160, Evalue=9e-40,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_MYCPN (P75476)

Other databases:

- EMBL:   U00089
- PIR:   S73860
- RefSeq:   NP_109990.1
- ProteinModelPortal:   P75476
- SMR:   P75476
- IntAct:   P75476
- GeneID:   877241
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN302
- HOGENOM:   HBG405044
- ProtClustDB:   PRK03202
- BioCyc:   MetaCyc:MONOMER-545
- BioCyc:   MPNE272634:MPN302-MONOMER
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35990; Mature: 35859

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 132-132 BINDING 167-167 BINDING 258-258 BINDING 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQL
CCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCEEEEECCCCCCHHHC
DLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSEL
CCCCCHHHHHHEECCHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHC
GVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGD
CCEEEEECCCCCCCCCCCCEEEHHHHHHHHHEEEEEEEEEECCCCCEEEHHHHHHCCCCC
LAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAI
EEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCHHHHH
KQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVP
HHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHCCCCCCC
VNYDFDQAFNMPPVDRSALIAVINKNII
EECCHHHCCCCCCCCHHHEEEEECCCCC
>Mature Secondary Structure 
SPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQL
CCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCEEEEECCCCCCHHHC
DLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSEL
CCCCCHHHHHHEECCHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHC
GVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGD
CCEEEEECCCCCCCCCCCCEEEHHHHHHHHHEEEEEEEEEECCCCCEEEHHHHHHCCCCC
LAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAI
EEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCHHHHH
KQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVP
HHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHCCCCCCC
VNYDFDQAFNMPPVDRSALIAVINKNII
EECCHHHCCCCCCCCHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633