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The map label for this gene is map

Identifier: 13507925

GI number: 13507925

Start: 229597

End: 230343

Strand: Direct

Name: map

Synonym: MPN186

Alternate gene names: 13507925

Gene position: 229597-230343 (Clockwise)

Preceding gene: 13507924

Following gene: 13507926

Centisome position: 28.12

GC content: 41.77

Gene sequence:

>747_bases
ATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTT
TACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAA
CTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCG
GACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGC
TTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGG
TGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTAT
TACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCC
TGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAA
TGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACA
AGTGGATTTGAATGTTTAACTAATTAA

Upstream 100 bases:

>100_bases
CCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTG
GTTCAACAGTTAGTGCATTA

Downstream 100 bases:

>100_bases
TGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGAC
AAGTTTCGCGTGTTGTTGGA

Product: methionine amino peptidase

Products: NA

Alternate protein names: MAP; Peptidase M

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIA
DNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGY
YLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITT
SGFECLTN

Sequences:

>Translated_248_residues
MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIA
DNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGY
YLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITT
SGFECLTN
>Mature_248_residues
MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIA
DNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGY
YLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITT
SGFECLTN

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI40385867, Length=252, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=23.8866396761134, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1786364, Length=248, Percent_Identity=26.2096774193548, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788728, Length=216, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71996291, Length=251, Percent_Identity=26.2948207171315, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323273, Length=240, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355531, Length=239, Percent_Identity=24.2677824267782, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583427, Length=236, Percent_Identity=27.5423728813559, Blast_Score=87, Evalue=9e-18,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM_MYCPN (Q11132)

Other databases:

- EMBL:   U34795
- EMBL:   U00089
- PIR:   S62822
- RefSeq:   NP_109874.1
- ProteinModelPortal:   Q11132
- GeneID:   876883
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN186
- HOGENOM:   HBG299384
- OMA:   AHAIPRK
- ProtClustDB:   CLSK335172
- BioCyc:   MPNE272634:MPN186-MONOMER
- BRENDA:   3.4.11.18
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 27657; Mature: 27657

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00680 MAP_1

Important sites: BINDING 77-77 BINDING 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKY
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEC
QNFPGFNCLSLNETVIHGIADNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQ
CCCCCCEEEECCHHHEEECCCCEEEECCCEEEEEEECCCCCEEEEEEHEECCCCCCHHHH
TLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGYYLLKQFGGHGCGIKVHEEPL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCE
ILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITT
EEECCCCCCCCCCCCCEEEEECCEEEECCCEEEEECCCCCEECCCCCCCEEEEEEEEEEE
SGFECLTN
CCCHHHCC
>Mature Secondary Structure
MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKY
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEC
QNFPGFNCLSLNETVIHGIADNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQ
CCCCCCEEEECCHHHEEECCCCEEEECCCEEEEEEECCCCCEEEEEEHEECCCCCCHHHH
TLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGYYLLKQFGGHGCGIKVHEEPL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCE
ILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITT
EEECCCCCCCCCCCCCEEEEECCEEEECCCEEEEECCCCCEECCCCCCCEEEEEEEEEEE
SGFECLTN
CCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8604303; 8948633