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The map label for this gene is metX [H]

Identifier: 13507799

GI number: 13507799

Start: 76186

End: 77337

Strand: Direct

Name: metX [H]

Synonym: MPN060

Alternate gene names: 13507799

Gene position: 76186-77337 (Clockwise)

Preceding gene: 13507798

Following gene: 13507801

Centisome position: 9.33

GC content: 42.97

Gene sequence:

>1152_bases
ATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTG
TGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTT
CCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGAAAGGTATTA
AATCGGTTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGT
GCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGC
CCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGAC
ATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCA
GGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATG
ATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGA
CGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGT
CGACCGCACTGGAGCTTACTTTGCGCGTTTTATTGCTAAACACATTGTTAGTTTGGGTTGAGCGGAAGAGTGTGAAGTGA
GTATTAGCTGGGTCTTTTCCCAACCATTACCACAATCAATTCAGGTCAAATGCTTTAACATTAATAAGGAATTTAGTGAA
CAATTAATAAACCAAGTTATAAGTCAATATTTCAATTGATCTGTTGCTAAAATTATTGCAAAGTTAAAACTCCTTGATCA
AGTTGAGTACTTTAGGTATGCTGTTTACGGTCACTTTGGTCACCAAACAGCCCCTTGAGAACAACTAAGTGAACGTGACT
CGCTGCAATGTTTAATCAAAAATTTCCAATAA

Upstream 100 bases:

>100_bases
GAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTT
TTATGCTAATCTTTTAATTA

Downstream 100 bases:

>100_bases
AAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCA
GAACTCAAGAAAACCAAAAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTGWKVL
NRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHD
MKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTG
RKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGWAEECEVSISWVFSQPLPQSIQVKCFNINKEFSE
QLINQVISQYFNWSVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAPWEQLSERDSLQCLIKNFQ

Sequences:

>Translated_383_residues
MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTG*KVL
NRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHD
MKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTG
RKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLG*AEECEVSISWVFSQPLPQSIQVKCFNINKEFSE
QLINQVISQYFN*SVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAP*EQLSERDSLQCLIKNFQ
>Mature_382_residues
AKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTG*KVLN
RLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDM
KSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGR
KIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLG*AEECEVSISWVFSQPLPQSIQVKCFNINKEFSEQ
LINQVISQYFN*SVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAP*EQLSERDSLQCLIKNFQ

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=37.6, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=37.3333333333333, Blast_Score=235, Evalue=6e-62,
Organism=Escherichia coli, GI1789311, Length=374, Percent_Identity=42.2459893048128, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17538498, Length=369, Percent_Identity=40.650406504065, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17551082, Length=370, Percent_Identity=39.7297297297297, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17538494, Length=369, Percent_Identity=39.8373983739837, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI25145633, Length=375, Percent_Identity=38.9333333333333, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32565905, Length=361, Percent_Identity=37.3961218836565, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=40, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25145635, Length=331, Percent_Identity=38.0664652567976, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI32565903, Length=295, Percent_Identity=39.6610169491525, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=40.2298850574713, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=369, Percent_Identity=37.1273712737127, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6320710, Length=366, Percent_Identity=37.1584699453552, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45552159, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580529, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580537, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580533, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580535, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580531, Length=364, Percent_Identity=39.8351648351648, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24580527, Length=364, Percent_Identity=39.2857142857143, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24580525, Length=364, Percent_Identity=39.2857142857143, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24580541, Length=354, Percent_Identity=38.7005649717514, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24580539, Length=116, Percent_Identity=39.6551724137931, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41744; Mature: 41613

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMV
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCEEEE
GGEILTTGYVDVVQTGKVLNRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGIT
CCHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCE
VGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLM
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCEEEHHHHHH
SVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCCCCCCCCCC
KIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGAEECEVSISWVFSQP
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCC
LPQSIQVKCFNINKEFSEQLINQVISQYFNSVAKIIAKLKLLDQVEYFRYAVYGHFGHQT
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APEQLSERDSLQCLIKNFQ
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMV
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCEEEE
GGEILTTGYVDVVQTGKVLNRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGIT
CCHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCE
VGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLM
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCEEEHHHHHH
SVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCCCCCCCCCC
KIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGAEECEVSISWVFSQP
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCC
LPQSIQVKCFNINKEFSEQLINQVISQYFNSVAKIIAKLKLLDQVEYFRYAVYGHFGHQT
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APEQLSERDSLQCLIKNFQ
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]