Click here to switch to the map view.

The map label for this gene is tsr [H]

Identifier: 13507764

GI number: 13507764

Start: 30244

End: 31110

Strand: Direct

Name: tsr [H]

Synonym: MPN025

Alternate gene names: 13507764

Gene position: 30244-31110 (Clockwise)

Preceding gene: 13507763

Following gene: 13507765

Centisome position: 3.7

GC content: 43.83

Gene sequence:

>867_bases
ATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTA
TGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAA
AGTATATTTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCA
CTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTC
GCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAAC
TAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAA
ATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGAAAGGGTTT
AAACTATGAACTAATTGAAGCGATTGCTAAAGCGACTAACTTACCCTTAGTGCTCCATGGTGGTTCGGGAATTCCGGAAG
CGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCG
ATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACC
TACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAG

Upstream 100 bases:

>100_bases
AAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGAT
TTTCGCGACGATAACCAATA

Downstream 100 bases:

>100_bases
ATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGA
AATAGCTAACTATCCCTTTG

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQVVVPMVKGLVDALKITVPVA
LHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQ
IATLKPDALAAGIGNIHGLYPDNWKGLNYELIEAIAKATNLPLVLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKA
IREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA

Sequences:

>Translated_288_residues
MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQVVVPMVKGLVDALKITVPVA
LHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQ
IATLKPDALAAGIGNIHGLYPDN*KGLNYELIEAIAKATNLPLVLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKA
IREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA
>Mature_288_residues
MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQVVVPMVKGLVDALKITVPVA
LHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQ
IATLKPDALAAGIGNIHGLYPDN*KGLNYELIEAIAKATNLPLVLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKA
IREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=292, Percent_Identity=33.9041095890411, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI87082041, Length=291, Percent_Identity=34.020618556701, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1788072, Length=292, Percent_Identity=36.3013698630137, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789293, Length=333, Percent_Identity=28.5285285285285, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322790, Length=342, Percent_Identity=28.3625730994152, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 30865; Mature: 30865

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQ
CCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCEEHHCCCE
VVVPMVKGLVDALKITVPVALHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKE
EHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHH
LIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQIATLKPDALAAGIGNIHGLY
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCC
PDNKGLNYELIEAIAKATNLPLVLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKAI
CCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHH
REYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA
HHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYISGHQ
CCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCEEHHCCCE
VVVPMVKGLVDALKITVPVALHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKE
EHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHH
LIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQIATLKPDALAAGIGNIHGLY
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCC
PDNKGLNYELIEAIAKATNLPLVLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKAI
CCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHH
REYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA
HHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]