Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 13507747

GI number: 13507747

Start: 9947

End: 11275

Strand: Direct

Name: trmE

Synonym: MPN008

Alternate gene names: 13507747

Gene position: 9947-11275 (Clockwise)

Preceding gene: 13507746

Following gene: 13507748

Centisome position: 1.22

GC content: 41.91

Gene sequence:

>1329_bases
ATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCC
CGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAA
TTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTG
ATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGC
CCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAG
TACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTA
GTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACT
GGTGACAATGAACCAAGCAGTCGTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGC
TCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAA
GATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGCGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGT
CAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCA
TTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAG
AAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAA
ACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCT
ACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAAC
TGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAA
CTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAA

Upstream 100 bases:

>100_bases
AAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGC
TAACCTCATTAACTGATAAG

Downstream 100 bases:

>100_bases
TGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGG
TTAATTGGGTTAAAGGCCAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDL
IEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPL
VEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQ
DKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLK
KLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQN
CLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK

Sequences:

>Translated_442_residues
MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDL
IEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPL
VEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQ
DKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLK
KLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQN
CLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK
>Mature_442_residues
MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDL
IEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPL
VEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQ
DKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLK
KLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQN
CLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=486, Percent_Identity=25.7201646090535, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI193082993, Length=475, Percent_Identity=25.4736842105263, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI193082988, Length=516, Percent_Identity=24.2248062015504, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=30.6209850107066, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082120, Length=130, Percent_Identity=33.0769230769231, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=456, Percent_Identity=27.1929824561404, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=28.7698412698413, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=26.0593220338983, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_MYCPN (P75104)

Other databases:

- EMBL:   U00089
- PIR:   S73472
- RefSeq:   NP_109696.1
- ProteinModelPortal:   P75104
- SMR:   P75104
- IntAct:   P75104
- GeneID:   877349
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN008
- HOGENOM:   HBG746795
- OMA:   VVEIACH
- ProtClustDB:   PRK05291
- BioCyc:   MPNE272634:MPN008-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49781; Mature: 49781

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 82-82 BINDING 121-121 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKK
CCCHHHHHHEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHH
VDDVLLFKFVAPNSYTGEDLIEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNG
HHHHHEEEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCHHCCCCCC
KMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPLVEKLGQLVMQMEVNLDYPEY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
DKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLV
HHHHHHHHCCCCCHHCCCCEEECCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCEE
IYLLDARQPEPDPKIIARLKKLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQ
EEEEECCCCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHEEEHHHHHHHHHHHHHHHH
YLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQNCLFFDILVVHLRAAHEYILQ
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VLGKNTNYDLINEIFKHFCLGK
HHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKK
CCCHHHHHHEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHH
VDDVLLFKFVAPNSYTGEDLIEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNG
HHHHHEEEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCHHCCCCCC
KMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPLVEKLGQLVMQMEVNLDYPEY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TDEQRELVTMNQAVVQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
DKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLV
HHHHHHHHCCCCCHHCCCCEEECCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCEE
IYLLDARQPEPDPKIIARLKKLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQ
EEEEECCCCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHEEEHHHHHHHHHHHHHHHH
YLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQNCLFFDILVVHLRAAHEYILQ
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VLGKNTNYDLINEIFKHFCLGK
HHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633