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The map label for this gene is holB [C]

Identifier: 13507746

GI number: 13507746

Start: 9184

End: 9945

Strand: Direct

Name: holB [C]

Synonym: MPN007

Alternate gene names: 13507746

Gene position: 9184-9945 (Clockwise)

Preceding gene: 13507745

Following gene: 13507747

Centisome position: 1.12

GC content: 42.52

Gene sequence:

>762_bases
ATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAAC
CCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACT
TTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAA
TCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAAT
TGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCG
TCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTC
TTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAA
TAAGAAAAACACAGCGAGCACGGTTCACGAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTAC
TGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAAC
CCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGATAA

Upstream 100 bases:

>100_bases
GCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAA
TTTATTGAACAATTAAGCCA

Downstream 100 bases:

>100_bases
GATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTC
CCGATGTTTATCGCATTATT

Product: DNA polymerase III subunit delta'

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSE
SNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSF
FSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFN
PPKAALFANLINW

Sequences:

>Translated_253_residues
MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSE
SNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSF
FSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFN
PPKAALFANLIN*
>Mature_253_residues
MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSE
SNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSF
FSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFN
PPKAALFANLIN*

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y007_MYCPN (P75105)

Other databases:

- EMBL:   U00089
- PIR:   S73473
- RefSeq:   NP_109695.1
- ProteinModelPortal:   P75105
- SMR:   P75105
- IntAct:   P75105
- GeneID:   877315
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN007
- HOGENOM:   HBG130191
- ProtClustDB:   PRK05818
- BioCyc:   MPNE272634:MPN007-MONOMER

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 28853; Mature: 28853

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
NPFKREHALHLSEVLNRQSESNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTT
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
KNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSFFSVDEQLQALDNGSFTRLKT
CCCCEEHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFN
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPKAALFANLIN
CCHHHHHHHHCC
>Mature Secondary Structure
MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
NPFKREHALHLSEVLNRQSESNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTT
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
KNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSFFSVDEQLQALDNGSFTRLKT
CCCCEEHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFN
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPKAALFANLIN
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633