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The map label for this gene is nadB [H]

Identifier: 13488071

GI number: 13488071

Start: 81171

End: 82715

Strand: Reverse

Name: nadB [H]

Synonym: mll9103

Alternate gene names: 13488071

Gene position: 82715-81171 (Counterclockwise)

Preceding gene: 13488072

Following gene: 13488070

Centisome position: 23.5

GC content: 65.57

Gene sequence:

>1545_bases
ATGAGCGCCGATGTCCGCAGCTTTTTTGGTCGACCCATCATCATTGGCGGTGGCATTGCCGGATTGTTGACCGCGCTTCA
TCTGGCGCCAGAACCCGTGCTTGTTTTGTCCAGGACGCCACTCGGATCTGAAGCATCGAGCGCGTGGGCACAGGGCGGGC
TGGCTGCCAGTCTTGGCATTGACGATAATCCGGCGCTGCACCTCGCCGATACGCTCGCCGCCGGCGATGGCCTGTGCGAC
GCAGACATCGCCAGCCGTATCCTCCATGCGGCCCCTAACGCGATTGAGGCCCTGGCAAACCTTGGGGTCTGCTTCGATCG
GACGCCAGAAGGAGCCCTGCGGCTTGGCTTGGAGGCCGCGCATGGCCGCCGGCGCATCGTTCATGCCGGCGGCGACGGCA
GCGGACAGGAAATCATGCGCGCGCTTGTCGAAACCGTGCGTTCCACGCCGTCGATTGTGGTCGTCGAGGGTGTGGAGGCG
CGGCGGCTGGCAGTGGAGGACAATACGGTCCGCGGCGTCTGGGCAAGCTGTTCGATGAGCCCTGTGTTCCTGGGTACGGG
ACGGGTGATCCTTGCCACCGGCGGCATTGGCGGACTGTTCTTCGACAGCACCAACCCTTCGGGGAGTTGCGGACAGGGCC
TGGGGCTTGCGGCCCGTGCCGGTGCTGTCCTTGCTGATCTCGAATTCGTCCAGTTCCACCCGACCGCGCTTGATGGGCCG
GCTCGCCCGATGCCGCTGATCAGCGAAGCGGTTCGCGGCGAAGGTGCTGTGATCGTCGACGAGACAGGCCGACGTTTTCT
CGAAGGCCTGGATGGGGCGGAACTCGCGCCGCGCGACGTGGTGGCGGGCGCCATCTGGACCCATCTTGCAAACGGCCACC
TCGTCTTTCTCGATGGCCGAGAAAAGCCCGGTCCGACATTCGCGCGGCAGTTTCCGACGATTGCATCAGCCTGCAGGCGC
GCAGGTATCGACCCCGCGCGCGATCTCATCCCCGTCCGGCCGGCCCAGCACTATCACATGGGCGGCGTCGCGGTGGACCA
GGATGGACGGACTTCCGTTGCCGGACTGTGGGCTTGCGGCGAAGTTGCCTCCACCGGACTGCACGGCGCCAACAGGCTCG
CCAGCAACTCGCTGACCGAGGCTGTGGTGTGCGCGCGCTGGGTCGCCGAAAGCGTGGCGGGATCTGCTATCGGCGTCAGG
ACACGATCGACGGCACGCGAAATTCCGGCGCCCGATCCAGTTTCCTTGCGCCCTCTCTTGACACGCACCCTTGGCGTGAG
CCGTGATGGCGCGATGTTGCGCGAGGCCATTCGGACATTGCTGCCGCTGGCCAAACGCCCCGATGCCACGTCCGATCCCG
CAGCAGTGGGACTGATGATCGCGGTTGCCGCCTTGCTGCGCCAGGAAAGCCGCGGCGCTCACTACCGCACGGACTTTCCC
GATCGTGCTGCCATCGCCCGCCGCTCCAGGCTCAGGCTTGATGAAGCCTTAGCGGCAGCCGACGAACTGGCATGTTCGCC
AACGCTTGAAGAGGTCGAGCCATGA

Upstream 100 bases:

>100_bases
CCAACATCCGTTCGGCCCTGGAGCAGAACCGCCACGTCGTGACCATCGAGCCCGGGATCGCCGGGCGCGCGCGACTTGCC
GTCGAGCGGATGCTCGCCGT

Downstream 100 bases:

>100_bases
ACCTCGCGCCCCTGCCCGCAATCATGCTCGAGCCCCTGGTCCGGGCAGCCCTTCTGGAAGATCTCGGCCGGGCGGGTGAT
CTGACCACCGACGCCATCGT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSADVRSFFGRPIIIGGGIAGLLTALHLAPEPVLVLSRTPLGSEASSAWAQGGLAASLGIDDNPALHLADTLAAGDGLCD
ADIASRILHAAPNAIEALANLGVCFDRTPEGALRLGLEAAHGRRRIVHAGGDGSGQEIMRALVETVRSTPSIVVVEGVEA
RRLAVEDNTVRGVWASCSMSPVFLGTGRVILATGGIGGLFFDSTNPSGSCGQGLGLAARAGAVLADLEFVQFHPTALDGP
ARPMPLISEAVRGEGAVIVDETGRRFLEGLDGAELAPRDVVAGAIWTHLANGHLVFLDGREKPGPTFARQFPTIASACRR
AGIDPARDLIPVRPAQHYHMGGVAVDQDGRTSVAGLWACGEVASTGLHGANRLASNSLTEAVVCARWVAESVAGSAIGVR
TRSTAREIPAPDPVSLRPLLTRTLGVSRDGAMLREAIRTLLPLAKRPDATSDPAAVGLMIAVAALLRQESRGAHYRTDFP
DRAAIARRSRLRLDEALAAADELACSPTLEEVEP

Sequences:

>Translated_514_residues
MSADVRSFFGRPIIIGGGIAGLLTALHLAPEPVLVLSRTPLGSEASSAWAQGGLAASLGIDDNPALHLADTLAAGDGLCD
ADIASRILHAAPNAIEALANLGVCFDRTPEGALRLGLEAAHGRRRIVHAGGDGSGQEIMRALVETVRSTPSIVVVEGVEA
RRLAVEDNTVRGVWASCSMSPVFLGTGRVILATGGIGGLFFDSTNPSGSCGQGLGLAARAGAVLADLEFVQFHPTALDGP
ARPMPLISEAVRGEGAVIVDETGRRFLEGLDGAELAPRDVVAGAIWTHLANGHLVFLDGREKPGPTFARQFPTIASACRR
AGIDPARDLIPVRPAQHYHMGGVAVDQDGRTSVAGLWACGEVASTGLHGANRLASNSLTEAVVCARWVAESVAGSAIGVR
TRSTAREIPAPDPVSLRPLLTRTLGVSRDGAMLREAIRTLLPLAKRPDATSDPAAVGLMIAVAALLRQESRGAHYRTDFP
DRAAIARRSRLRLDEALAAADELACSPTLEEVEP
>Mature_513_residues
SADVRSFFGRPIIIGGGIAGLLTALHLAPEPVLVLSRTPLGSEASSAWAQGGLAASLGIDDNPALHLADTLAAGDGLCDA
DIASRILHAAPNAIEALANLGVCFDRTPEGALRLGLEAAHGRRRIVHAGGDGSGQEIMRALVETVRSTPSIVVVEGVEAR
RLAVEDNTVRGVWASCSMSPVFLGTGRVILATGGIGGLFFDSTNPSGSCGQGLGLAARAGAVLADLEFVQFHPTALDGPA
RPMPLISEAVRGEGAVIVDETGRRFLEGLDGAELAPRDVVAGAIWTHLANGHLVFLDGREKPGPTFARQFPTIASACRRA
GIDPARDLIPVRPAQHYHMGGVAVDQDGRTSVAGLWACGEVASTGLHGANRLASNSLTEAVVCARWVAESVAGSAIGVRT
RSTAREIPAPDPVSLRPLLTRTLGVSRDGAMLREAIRTLLPLAKRPDATSDPAAVGLMIAVAALLRQESRGAHYRTDFPD
RAAIARRSRLRLDEALAAADELACSPTLEEVEP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=381, Percent_Identity=33.8582677165354, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788928, Length=512, Percent_Identity=36.9140625, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790597, Length=493, Percent_Identity=30.8316430020284, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786942, Length=513, Percent_Identity=30.0194931773879, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17550100, Length=503, Percent_Identity=30.4174950298211, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17505833, Length=513, Percent_Identity=29.8245614035088, Blast_Score=161, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71986328, Length=263, Percent_Identity=30.7984790874525, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=509, Percent_Identity=30.6483300589391, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322416, Length=514, Percent_Identity=28.4046692607004, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17137288, Length=504, Percent_Identity=31.7460317460317, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24655642, Length=504, Percent_Identity=31.7460317460317, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24655647, Length=504, Percent_Identity=31.7460317460317, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24663005, Length=505, Percent_Identity=27.7227722772277, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 53405; Mature: 53274

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADVRSFFGRPIIIGGGIAGLLTALHLAPEPVLVLSRTPLGSEASSAWAQGGLAASLGI
CCCCHHHHCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCEEEECCC
DDNPALHLADTLAAGDGLCDADIASRILHAAPNAIEALANLGVCFDRTPEGALRLGLEAA
CCCCCEEHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEECCCCCHHHHHCHHHC
HGRRRIVHAGGDGSGQEIMRALVETVRSTPSIVVVEGVEARRLAVEDNTVRGVWASCSMS
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEECCCCC
PVFLGTGRVILATGGIGGLFFDSTNPSGSCGQGLGLAARAGAVLADLEFVQFHPTALDGP
EEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHCCCEEEECCCCCCCC
ARPMPLISEAVRGEGAVIVDETGRRFLEGLDGAELAPRDVVAGAIWTHLANGHLVFLDGR
CCCCHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECC
EKPGPTFARQFPTIASACRRAGIDPARDLIPVRPAQHYHMGGVAVDQDGRTSVAGLWACG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECCCEEECCCCCCCHHHHHHHH
EVASTGLHGANRLASNSLTEAVVCARWVAESVAGSAIGVRTRSTAREIPAPDPVSLRPLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCHHHHH
TRTLGVSRDGAMLREAIRTLLPLAKRPDATSDPAAVGLMIAVAALLRQESRGAHYRTDFP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DRAAIARRSRLRLDEALAAADELACSPTLEEVEP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SADVRSFFGRPIIIGGGIAGLLTALHLAPEPVLVLSRTPLGSEASSAWAQGGLAASLGI
CCCHHHHCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCEEEECCC
DDNPALHLADTLAAGDGLCDADIASRILHAAPNAIEALANLGVCFDRTPEGALRLGLEAA
CCCCCEEHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEECCCCCHHHHHCHHHC
HGRRRIVHAGGDGSGQEIMRALVETVRSTPSIVVVEGVEARRLAVEDNTVRGVWASCSMS
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEECCCCC
PVFLGTGRVILATGGIGGLFFDSTNPSGSCGQGLGLAARAGAVLADLEFVQFHPTALDGP
EEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHCCCEEEECCCCCCCC
ARPMPLISEAVRGEGAVIVDETGRRFLEGLDGAELAPRDVVAGAIWTHLANGHLVFLDGR
CCCCHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECC
EKPGPTFARQFPTIASACRRAGIDPARDLIPVRPAQHYHMGGVAVDQDGRTSVAGLWACG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECCCEEECCCCCCCHHHHHHHH
EVASTGLHGANRLASNSLTEAVVCARWVAESVAGSAIGVRTRSTAREIPAPDPVSLRPLL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCHHHHH
TRTLGVSRDGAMLREAIRTLLPLAKRPDATSDPAAVGLMIAVAALLRQESRGAHYRTDFP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DRAAIARRSRLRLDEALAAADELACSPTLEEVEP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]