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The map label for this gene is ngoMIVM [H]

Identifier: 13476615

GI number: 13476615

Start: 6585084

End: 6586256

Strand: Direct

Name: ngoMIVM [H]

Synonym: mlr7992

Alternate gene names: 13476615

Gene position: 6585084-6586256 (Clockwise)

Preceding gene: 13476614

Following gene: 13476618

Centisome position: 93.59

GC content: 64.79

Gene sequence:

>1173_bases
GTGGCTCTATGTCCCGAACACATCGACATCCTCAGCCTCTGCACAGGAGGGGCGGGCCTCGATCTCGGCGTCGAGCTGGC
AATTCCGAGCGCTCGAACAGTCTGCATGGTGGAGAGGGAAGCCTTCGCCGTCGCGCACCTGGTTTCAGCGATGGAACAGG
GTCTACTTCATCCAGCGCCTATTTGGAGCGATGCCAGAACCTTCGACGGCCGCGCATGGCGTGGCTGCGTTGACGGCCTC
GTTGGCGGCATCCCGTGCCAGCCGCATTCCCTTGCCGGCAAGCGGCTCGGCGAGGGCGATGAGCGCGACCTGTGGTCGAC
GGCACGGCGCATCATCGTCCAGTCCGGCTGCTGGTTCGTCCTCATCGAAAACGTCGGCGGCATGCTCTCCAGCGGCGGCG
CGGAGCGCGTATGGCGAGACCTTCGCAGACTGGGCTTTGAGGTTGAGGGTGGACTGTTCACGGCGAAGGAAGTTGGCGCG
AGCCATGAGCGCGAACGCTTCTTCCTGCTCGGCGTGGCCGACGCCGGACGCCTTTGTCTCGAACGATGGCGAACTGCCGG
CGACCTGGTTGTCGCGGCAAGCGCTCGAAAAATCCAAGGGCAAAAACGGCAACGGCATGGGCCTGCCGCTGGCAATGGCG
GCGAAGTTGTGGCCGACGCCGGCCGCGCTCGACGGCCAGAAGGCACCGAAGACGTTCGCCGGCGGCAATATCAGCTTGCC
GGAGGCGGCCAAGACCTGGAGCACTCCAAGGGCCTCAGATGGCGAGAAGGGTGGGCCGAAACAGGCGTTCGGGGCGGGCG
GAATGCCATTGGCGGCGCAGACACTGCAATGGCGCACGCCGCGATCGAGCGAGTCGGGCCAGTATCAATACGATCGCGGC
GACAAGTCAAAGCCGACACCCACGCTGACAGGCCAGGCCTTTTCCCACCTGGGCCAACCGACATCGAAGCCTGGCGAAAC
GTGCTTGGTAGAGCGCCGCAGCTTGAACCCGCTGTTCGTCGAATGGCTGATGGGGTGGCCGCCCGGGTGGACCGCCTCAG
AATGCTCGGCAACGGCGTTGTCAGTCTGGAAGCGGCATATGCGCTGCGCACTCTCGCAACTCGCCTCGCCGGCCAAGGCT
CCGCCGGCGCAGCTGTCCTTATTCGCGTGATGGAGAGTTCAATTGCTGCCTAA

Upstream 100 bases:

>100_bases
AGATCGACCAGAAGCGAACCGAACTCCTCGTGCTCGGCACCATCGCCGAGGACTACGAGCGCGCCGTTGAGGTCACCAAG
ACGAGGGTGGCCGGGTGAAT

Downstream 100 bases:

>100_bases
GCCAGGAAATTTCGCGAAGACCACCACGATGCAACATCTTGTGGCAATTGCTGCAAACACAAGCGAGTTGGGCAAGGGTA
GTTCGTCTTCCTTCCTCAGA

Product: DNA modification methylase

Products: NA

Alternate protein names: M.NgoMIV; Cytosine-specific methyltransferase NgoMIV [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MALCPEHIDILSLCTGGAGLDLGVELAIPSARTVCMVEREAFAVAHLVSAMEQGLLHPAPIWSDARTFDGRAWRGCVDGL
VGGIPCQPHSLAGKRLGEGDERDLWSTARRIIVQSGCWFVLIENVGGMLSSGGAERVWRDLRRLGFEVEGGLFTAKEVGA
SHERERFFLLGVADAGRLCLERWRTAGDLVVAASARKIQGQKRQRHGPAAGNGGEVVADAGRARRPEGTEDVRRRQYQLA
GGGQDLEHSKGLRWREGWAETGVRGGRNAIGGADTAMAHAAIERVGPVSIRSRRQVKADTHADRPGLFPPGPTDIEAWRN
VLGRAPQLEPAVRRMADGVAARVDRLRMLGNGVVSLEAAYALRTLATRLAGQGSAGAAVLIRVMESSIAA

Sequences:

>Translated_390_residues
MALCPEHIDILSLCTGGAGLDLGVELAIPSARTVCMVEREAFAVAHLVSAMEQGLLHPAPIWSDARTFDGRAWRGCVDGL
VGGIPCQPHSLAGKRLGEGDERDLWSTARRIIVQSGCWFVLIENVGGMLSSGGAERVWRDLRRLGFEVEGGLFTAKEVGA
SHERERFFLLGVADAGRLCLERWRTAGDLVVAASARKIQGQKRQRHGPAAGNGGEVVADAGRARRPEGTEDVRRRQYQLA
GGGQDLEHSKGLRWREGWAETGVRGGRNAIGGADTAMAHAAIERVGPVSIRSRRQVKADTHADRPGLFPPGPTDIEAWRN
VLGRAPQLEPAVRRMADGVAARVDRLRMLGNGVVSLEAAYALRTLATRLAGQGSAGAAVLIRVMESSIAA
>Mature_389_residues
ALCPEHIDILSLCTGGAGLDLGVELAIPSARTVCMVEREAFAVAHLVSAMEQGLLHPAPIWSDARTFDGRAWRGCVDGLV
GGIPCQPHSLAGKRLGEGDERDLWSTARRIIVQSGCWFVLIENVGGMLSSGGAERVWRDLRRLGFEVEGGLFTAKEVGAS
HERERFFLLGVADAGRLCLERWRTAGDLVVAASARKIQGQKRQRHGPAAGNGGEVVADAGRARRPEGTEDVRRRQYQLAG
GGQDLEHSKGLRWREGWAETGVRGGRNAIGGADTAMAHAAIERVGPVSIRSRRQVKADTHADRPGLFPPGPTDIEAWRNV
LGRAPQLEPAVRRMADGVAARVDRLRMLGNGVVSLEAAYALRTLATRLAGQGSAGAAVLIRVMESSIAA

Specific function: This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 41726; Mature: 41595

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALCPEHIDILSLCTGGAGLDLGVELAIPSARTVCMVEREAFAVAHLVSAMEQGLLHPAP
CCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
IWSDARTFDGRAWRGCVDGLVGGIPCQPHSLAGKRLGEGDERDLWSTARRIIVQSGCWFV
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
LIENVGGMLSSGGAERVWRDLRRLGFEVEGGLFTAKEVGASHERERFFLLGVADAGRLCL
EECHHCCHHCCCCHHHHHHHHHHCCCEECCCEEEHHHHCCCCCCCEEEEEEECCHHHHHH
ERWRTAGDLVVAASARKIQGQKRQRHGPAAGNGGEVVADAGRARRPEGTEDVRRRQYQLA
HHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHC
GGGQDLEHSKGLRWREGWAETGVRGGRNAIGGADTAMAHAAIERVGPVSIRSRRQVKADT
CCCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHHCCC
HADRPGLFPPGPTDIEAWRNVLGRAPQLEPAVRRMADGVAARVDRLRMLGNGVVSLEAAY
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALRTLATRLAGQGSAGAAVLIRVMESSIAA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALCPEHIDILSLCTGGAGLDLGVELAIPSARTVCMVEREAFAVAHLVSAMEQGLLHPAP
CCCCHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
IWSDARTFDGRAWRGCVDGLVGGIPCQPHSLAGKRLGEGDERDLWSTARRIIVQSGCWFV
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
LIENVGGMLSSGGAERVWRDLRRLGFEVEGGLFTAKEVGASHERERFFLLGVADAGRLCL
EECHHCCHHCCCCHHHHHHHHHHCCCEECCCEEEHHHHCCCCCCCEEEEEEECCHHHHHH
ERWRTAGDLVVAASARKIQGQKRQRHGPAAGNGGEVVADAGRARRPEGTEDVRRRQYQLA
HHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHC
GGGQDLEHSKGLRWREGWAETGVRGGRNAIGGADTAMAHAAIERVGPVSIRSRRQVKADT
CCCCCCHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHHCCC
HADRPGLFPPGPTDIEAWRNVLGRAPQLEPAVRRMADGVAARVDRLRMLGNGVVSLEAAY
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALRTLATRLAGQGSAGAAVLIRVMESSIAA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1321116 [H]