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The map label for this gene is pcrA [H]

Identifier: 13476580

GI number: 13476580

Start: 6556921

End: 6559014

Strand: Direct

Name: pcrA [H]

Synonym: mlr7946

Alternate gene names: 13476580

Gene position: 6556921-6559014 (Clockwise)

Preceding gene: 13476579

Following gene: 13476581

Centisome position: 93.19

GC content: 65.57

Gene sequence:

>2094_bases
ATGAACCTCGCCGCCCATGATTCGACCTTTTTGGAATCGGCCGTCGCGCCCGCCTATCTCGCGCGGCTGAACGACGCCCA
GCGCCAGGCCGTCGAGCATGGCGACGGCAAGATCGCAGGGCCGTTGCTGGTCATTGCCGGCGCCGGCTCGGGCAAGACCA
ACACGCTGGCGCATCGCGTCGCCCATCTGATCGTCAAGGGGGCCGACCCGCGCCGCATCCTGCTGATGACCTTTTCGCGG
CGCGCCGCATCCGAAATGGCGAAACGTGTCGAGCGCATCGCCGGCGAGGTGCTCGGCCGCGACGCCGCGGTCATCACCGA
TGCGCTGACCTGGGCCGGCACTTTTCACGGCATCGGCGCCCGGCTGTTGCGCGACTATGCGCTGGAGATCGGCCTCGACC
CGGCCTTCACCATTCACGACCGCGAAGATTCCGCCGATCTGATGAACCTGGCGCGCCACGAGCTGGGTTTCTCGAAGACC
GAAGCGCGCTTTCCGACCAAGGGCACTTGCCTTGCCATCTATTCGCGCGCCGTCAACGCGCAGGCGCCGCTCAATGAAGT
GCTTGGTTCCGCCTTTCCCTGGTGTGCCGGCTGGGCCGAGCAGTTGAAGCAATTGTTCGCCGGATATGTCGAAGCCAAGC
AGGCGCAGAACGTGCTCGATTACGACGACCTGCTGCTCTACTGGGCGCAGATGACGGCCGAGCCGGAGATCGCGGAGCAT
CTGGGTTCGCGTTTCGACCATGTGCTGGTCGACGAGTATCAGGACACCAATCGCCTGCAGGCCTCGATCCTGATGGCGCT
GAAACCCGATGGCGCCGGGCTGACGGTGGTCGGCGACGATGCGCAGTCGATCTATTCCTTCCGCGCCGCCGAGGTGCGCA
ACATTCTCGATTTTCCCCGGCAGTTCGCGCAGGCAGCCGATGTGGTGATGCTGGAGCGCAATTACCGCTCGACGGAAACC
ATTCTGGCCGCTGCCAATGCAGTGATCGGCGAGGCCTCGGAGCGCTTCACCAAGAACCTTTGGTCGGAGCGCAAATCCAC
CGACAAGCCAAGGCTGGTGAGCGTCCGCGACGAGGTCGAGCAGGCAAACTTTGTCTGCGATACCATCCTCGCCGAGCGCG
AGGCCGGTACGGCACTGAAATCGCAGGCAGTGCTGTTTCGCGCCTCGCACCACAGCGGGCCGCTGGAGATCGAACTGACG
CGGCGCAACATTCCCTTCGTCAAATTCGGCGGCCTGAAATTCCTCGATGCCGCCCATGTCAAGGATGTGCTGGCGGTGCT
GCGTTTCGCCGAAAACCCGCGCGACCGCGTCGCCGGCTTTCGCGTCCTGCAACTGATGCCGGGCATTGGCCCTTCCGCCG
CCGCGCAGATCGTCGAGACGATGACGACGGCGCTTGACGAGGCGATGGGGCTGGCCGGCTGGCGGCCGCCGCAACGCGCG
GCGGACGACTGGCCGGGCTTCGTCTCGCTCTATTCGGGCCTGCGGGCCGGCGCCAAATGGCCGGCCGATCTCGAACAGGT
CAGGCTCTGGTACGAGCCGCATCTGGAACGCATCCATGAGGATGCCATCACACGCCGCGCCGACTTGTTGCAGCTGGAGC
AGATCGGCTCGGGCTACGCCTCGCGCGAACGCTTCCTGACCGAACTCACGCTCGATCCGCCGGATGCGACCAGCGACCAG
GCTGGACCGCCGCATCGCGATGAGGACTATCTGATTCTCTCGACCATCCACTCGGCCAAGGGCCAGGAGTGGAAGAACGT
CTTCGTGCTCAACACCGTCGATGGCTGCATTCCGGCCGATCTCGGCGTCGGCACCAAGGAGGACATCGAGGAGGAGCGCC
GGCTGCTCTATGTGGCAATGACGCGGGCCAAGGACAGCCTCAACCTGGTCATGCCGCAGCGCTTCTTTCCGCATGGCCAG
GCCGCGCGCGGCGACCGTCACCTCTACGCCTCGCGCACCCGCTTCATCCCCGCCTCGATCCTCTCCGCGTTCCAGCAGAT
GTCGTGGCCGGGCGCACAGGCGGCACAGGGCAGGGCGCCTCGGCCGGACGTGCGCGTCGACATCGGCGCGCGCATGCGCG
GCATGTGGAAATAG

Upstream 100 bases:

>100_bases
AAAGGCCTGTAGCGTTCGCCCTTGGGAATTGCGTCAAAGCTAAGGGTCCGTCGGGGCGCTTCGGTGTTGCGAACAAAGCG
GAAACGATGCTTGATGGGCG

Downstream 100 bases:

>100_bases
CTGAAATCATGGCGCAACCGCCGATCAGGATCGCCATCGCCGGGGCGCTGGGCCGCATGGGCCGCCAGATGGTGGAGGCT
GTGCAGGCAGATCCGCGGCT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNLAAHDSTFLESAVAPAYLARLNDAQRQAVEHGDGKIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSR
RAASEMAKRVERIAGEVLGRDAAVITDALTWAGTFHGIGARLLRDYALEIGLDPAFTIHDREDSADLMNLARHELGFSKT
EARFPTKGTCLAIYSRAVNAQAPLNEVLGSAFPWCAGWAEQLKQLFAGYVEAKQAQNVLDYDDLLLYWAQMTAEPEIAEH
LGSRFDHVLVDEYQDTNRLQASILMALKPDGAGLTVVGDDAQSIYSFRAAEVRNILDFPRQFAQAADVVMLERNYRSTET
ILAAANAVIGEASERFTKNLWSERKSTDKPRLVSVRDEVEQANFVCDTILAEREAGTALKSQAVLFRASHHSGPLEIELT
RRNIPFVKFGGLKFLDAAHVKDVLAVLRFAENPRDRVAGFRVLQLMPGIGPSAAAQIVETMTTALDEAMGLAGWRPPQRA
ADDWPGFVSLYSGLRAGAKWPADLEQVRLWYEPHLERIHEDAITRRADLLQLEQIGSGYASRERFLTELTLDPPDATSDQ
AGPPHRDEDYLILSTIHSAKGQEWKNVFVLNTVDGCIPADLGVGTKEDIEEERRLLYVAMTRAKDSLNLVMPQRFFPHGQ
AARGDRHLYASRTRFIPASILSAFQQMSWPGAQAAQGRAPRPDVRVDIGARMRGMWK

Sequences:

>Translated_697_residues
MNLAAHDSTFLESAVAPAYLARLNDAQRQAVEHGDGKIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSR
RAASEMAKRVERIAGEVLGRDAAVITDALTWAGTFHGIGARLLRDYALEIGLDPAFTIHDREDSADLMNLARHELGFSKT
EARFPTKGTCLAIYSRAVNAQAPLNEVLGSAFPWCAGWAEQLKQLFAGYVEAKQAQNVLDYDDLLLYWAQMTAEPEIAEH
LGSRFDHVLVDEYQDTNRLQASILMALKPDGAGLTVVGDDAQSIYSFRAAEVRNILDFPRQFAQAADVVMLERNYRSTET
ILAAANAVIGEASERFTKNLWSERKSTDKPRLVSVRDEVEQANFVCDTILAEREAGTALKSQAVLFRASHHSGPLEIELT
RRNIPFVKFGGLKFLDAAHVKDVLAVLRFAENPRDRVAGFRVLQLMPGIGPSAAAQIVETMTTALDEAMGLAGWRPPQRA
ADDWPGFVSLYSGLRAGAKWPADLEQVRLWYEPHLERIHEDAITRRADLLQLEQIGSGYASRERFLTELTLDPPDATSDQ
AGPPHRDEDYLILSTIHSAKGQEWKNVFVLNTVDGCIPADLGVGTKEDIEEERRLLYVAMTRAKDSLNLVMPQRFFPHGQ
AARGDRHLYASRTRFIPASILSAFQQMSWPGAQAAQGRAPRPDVRVDIGARMRGMWK
>Mature_697_residues
MNLAAHDSTFLESAVAPAYLARLNDAQRQAVEHGDGKIAGPLLVIAGAGSGKTNTLAHRVAHLIVKGADPRRILLMTFSR
RAASEMAKRVERIAGEVLGRDAAVITDALTWAGTFHGIGARLLRDYALEIGLDPAFTIHDREDSADLMNLARHELGFSKT
EARFPTKGTCLAIYSRAVNAQAPLNEVLGSAFPWCAGWAEQLKQLFAGYVEAKQAQNVLDYDDLLLYWAQMTAEPEIAEH
LGSRFDHVLVDEYQDTNRLQASILMALKPDGAGLTVVGDDAQSIYSFRAAEVRNILDFPRQFAQAADVVMLERNYRSTET
ILAAANAVIGEASERFTKNLWSERKSTDKPRLVSVRDEVEQANFVCDTILAEREAGTALKSQAVLFRASHHSGPLEIELT
RRNIPFVKFGGLKFLDAAHVKDVLAVLRFAENPRDRVAGFRVLQLMPGIGPSAAAQIVETMTTALDEAMGLAGWRPPQRA
ADDWPGFVSLYSGLRAGAKWPADLEQVRLWYEPHLERIHEDAITRRADLLQLEQIGSGYASRERFLTELTLDPPDATSDQ
AGPPHRDEDYLILSTIHSAKGQEWKNVFVLNTVDGCIPADLGVGTKEDIEEERRLLYVAMTRAKDSLNLVMPQRFFPHGQ
AARGDRHLYASRTRFIPASILSAFQQMSWPGAQAAQGRAPRPDVRVDIGARMRGMWK

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=668, Percent_Identity=31.437125748503, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=29.1044776119403, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322369, Length=473, Percent_Identity=27.6955602536998, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77046; Mature: 77046

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAAHDSTFLESAVAPAYLARLNDAQRQAVEHGDGKIAGPLLVIAGAGSGKTNTLAHRV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCCHHHHHHHH
AHLIVKGADPRRILLMTFSRRAASEMAKRVERIAGEVLGRDAAVITDALTWAGTFHGIGA
HHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RLLRDYALEIGLDPAFTIHDREDSADLMNLARHELGFSKTEARFPTKGTCLAIYSRAVNA
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
QAPLNEVLGSAFPWCAGWAEQLKQLFAGYVEAKQAQNVLDYDDLLLYWAQMTAEPEIAEH
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
LGSRFDHVLVDEYQDTNRLQASILMALKPDGAGLTVVGDDAQSIYSFRAAEVRNILDFPR
HHHHCCHHHHHCCCCHHHHEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QFAQAADVVMLERNYRSTETILAAANAVIGEASERFTKNLWSERKSTDKPRLVSVRDEVE
HHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
QANFVCDTILAEREAGTALKSQAVLFRASHHSGPLEIELTRRNIPFVKFGGLKFLDAAHV
HHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHH
KDVLAVLRFAENPRDRVAGFRVLQLMPGIGPSAAAQIVETMTTALDEAMGLAGWRPPQRA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADDWPGFVSLYSGLRAGAKWPADLEQVRLWYEPHLERIHEDAITRRADLLQLEQIGSGYA
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
SRERFLTELTLDPPDATSDQAGPPHRDEDYLILSTIHSAKGQEWKNVFVLNTVDGCIPAD
HHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCEEEEECCCCCCCCC
LGVGTKEDIEEERRLLYVAMTRAKDSLNLVMPQRFFPHGQAARGDRHLYASRTRFIPASI
CCCCCHHHHHHHHHEEEEEEHHCCCCCCEECCHHHCCCCCCCCCCCHHHHHHHCCCHHHH
LSAFQQMSWPGAQAAQGRAPRPDVRVDIGARMRGMWK
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MNLAAHDSTFLESAVAPAYLARLNDAQRQAVEHGDGKIAGPLLVIAGAGSGKTNTLAHRV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCCHHHHHHHH
AHLIVKGADPRRILLMTFSRRAASEMAKRVERIAGEVLGRDAAVITDALTWAGTFHGIGA
HHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RLLRDYALEIGLDPAFTIHDREDSADLMNLARHELGFSKTEARFPTKGTCLAIYSRAVNA
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
QAPLNEVLGSAFPWCAGWAEQLKQLFAGYVEAKQAQNVLDYDDLLLYWAQMTAEPEIAEH
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
LGSRFDHVLVDEYQDTNRLQASILMALKPDGAGLTVVGDDAQSIYSFRAAEVRNILDFPR
HHHHCCHHHHHCCCCHHHHEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QFAQAADVVMLERNYRSTETILAAANAVIGEASERFTKNLWSERKSTDKPRLVSVRDEVE
HHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
QANFVCDTILAEREAGTALKSQAVLFRASHHSGPLEIELTRRNIPFVKFGGLKFLDAAHV
HHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHH
KDVLAVLRFAENPRDRVAGFRVLQLMPGIGPSAAAQIVETMTTALDEAMGLAGWRPPQRA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADDWPGFVSLYSGLRAGAKWPADLEQVRLWYEPHLERIHEDAITRRADLLQLEQIGSGYA
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
SRERFLTELTLDPPDATSDQAGPPHRDEDYLILSTIHSAKGQEWKNVFVLNTVDGCIPAD
HHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCEEEEECCCCCCCCC
LGVGTKEDIEEERRLLYVAMTRAKDSLNLVMPQRFFPHGQAARGDRHLYASRTRFIPASI
CCCCCHHHHHHHHHEEEEEEHHCCCCCCEECCHHHCCCCCCCCCCCHHHHHHHCCCHHHH
LSAFQQMSWPGAQAAQGRAPRPDVRVDIGARMRGMWK
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]