Click here to switch to the map view.

The map label for this gene is mrcA [H]

Identifier: 13476361

GI number: 13476361

Start: 6339500

End: 6341653

Strand: Reverse

Name: mrcA [H]

Synonym: mll7657

Alternate gene names: 13476361

Gene position: 6341653-6339500 (Counterclockwise)

Preceding gene: 13476362

Following gene: 13476360

Centisome position: 90.13

GC content: 63.88

Gene sequence:

>2154_bases
ATGGTCAGCCCTGAACCGCGCAAGAGAAGAGGTCCGATCGCGTCGCGCCTTCTGGCGCTGGACGCCTGGATCGATTCCTC
GCTCTACGAGATCGGGTTCAAGGCGCGGCAGTTCTGGGAAGCGGCGACGATCTTCTCGCGGCGCTTTCGGCTCAAGGGCT
GGCGGCGCGGCATCATCGAGATTTTGAGCGAAACTTTCACGCTCGGCGCCGGCGGCATCGTGGTGATGCTGGCGCTGGCC
ATCCCGGCGTTCCAGGATACGGCCGGTGACTGGCGCGCCCAGGGCGATTTCGCCGTTACCTTCCTCGACCGCTATGGCAA
CGAGATCGGCCAGCGCGGCATCATCCAGCGCGATTCCGTGCCGGTCGACGAGATGCCCGATCACGTCATCAAGGCGGTAC
TGGCCACCGAGGACCGGCGTTTCTTCGACCACTACGGCATCGACGTGCTCGGCCTGTCACGCGCGATCTTCGAGAATGTG
CGGGCGAACTCGGTCGTCCAGGGTGGCTCGAGCATCACTCAGCAATTGGCCAAGAACCTGTTCCTGACCAATGAGCGCAC
CTTCGAGCGCAAGATCAAGGAAGCCTTCCTGTCGCTGTGGCTCGAAGCCAATCTGTCGAAGAAGGAAATCCTGCAGCTGT
ATCTCGACCGCGCCTATATGGGCGGCGGCACGTTCGGCATCGAGGCGGCGGCGGACTTCTATTTCGGCAAGAGCGTCAAG
GATCTTAATCTCGCCGAGGCGGCGATGCTGGCCGGCCTGTTCAAGGCGCCGACCAAATACGCCCCCCACATCAACCTGCC
GGCGGCACGCGCCCGCGCCAATGTGGTGTTGTCGAACCTCGTCGATTCCGGCTTCATGACCGAAGGCCAGGTGCTGCAGG
CAAGGCTGCATCCGGCCGACGTCGTCGATCGCGGCGAACAGAAAAGCCCCGACTATTTCCTCGACTGGGCCTTCGACGAG
GTCAAGAAGATCGCCAAGCCGGGCCAGCATTCGCTGGTTGCCCACACTACCTTCGACGCCAACATCCAGAAGGCGGCGGA
AGAGTCCGTCGAGTTCCATCTGCGCCAGTTCGGCAAGGAGTACAACGTCACCGAAGGTGCCGTGGTGGTCATCGAGACCA
ATGGCGCGGTGCGGGCGATCGTCGGCGGCCGCGACTACGGCGCCAGCCAGTTCAACCGCGCCACCAAGGCGCTGCGTCAG
ACCGGTTCCTCCTTCAAGCCCTATGTCTACGCCACGGCGATGGAGCACGGCTTTACGCCCAACTCAATCATTTCCGGCGG
GCCAATCAGCTGGGGCAACTGGTCACCGCACAATTATAGCGGCGAGTCGGCTGGCAACATCACGCTGATCATGGCGATGG
CCAAGTCGATCAACACCGTGCCGGTGCGGCTGGCCAAGGACCATCTCGGCATCGCGCCGATCAAGGCGATGGCCGAGGCG
ATGGGCGTTGAATCGCCGCTCGAGTCGCACAAGACGATGGTGCTCGGCACTTCGGGCATGACCGTGATGGACCAGGCGAC
GGGCTACAGCGTGTTCGCCCAGAACGGCTTTGTCGGCTCCCGGCACGGCATCACCCAGCTCGTCACCCGCACCGGCGACG
TGGTGTATGACTGGACCAAGGACGCGACGCCGCCGCACCGGGTGCTGTCCGAGCAGGCACTGAAATCCATGAACACCATG
CTGGCGGCCGTGCCGGTGATGGGCACGGCGCGGCGCGCGCAAATTCCCAACATCGTCGTCGCTGGCAAAACCGGCACGAC
GCAATCCTACCGTGATGCCTGGTTCGTCGGCTTCACCGGCAACTACACGGCAGCCGTGTGGCTGGGCAACGACGATTTCA
CCCCGACCAAGAACATGACGGGCGGCTCGCTGCCGGCGATGGTGTGGCAGCGCCTGATGGTCTACGCGCACCAGAACATC
GACCTGAAGCCGATCCCCGGCATCGACAAACCTTTCGTCGACGAGGATATCGCGGCCAAGGCCGCTGAAGCGCAGAAGAA
GAGCGAGGATGAAGCCGCGGCCGACGCGGCAGCCGAACGCCCGGCGGTGCTGTCCAGCCGAACCACGCAGACGCTGAGGG
ACATGACGCAGCTGTTCCAGTCCGCCCCTAAACTCGACGCCGCCGCCTCGCCCGAAACGCTCTCGGCGCTGTGA

Upstream 100 bases:

>100_bases
TGGGCACCCATAAAATGGATGAATAGTCACGACAGGGCGCTGCCCTGAAATTGCCGGCTTGCGGTTTTCAGGTCACGCCA
CAGCCGAGCCGGGTTTTTGA

Downstream 100 bases:

>100_bases
CTTCGGCCTCGGCGCGGACCAAGCCAACAGGCAAATCCATCACGCTCGCTTGCCACAAGGCCCCGCGCCGCGATATCCCC
GAACGGCAATCCTTCGCGCA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MVSPEPRKRRGPIASRLLALDAWIDSSLYEIGFKARQFWEAATIFSRRFRLKGWRRGIIEILSETFTLGAGGIVVMLALA
IPAFQDTAGDWRAQGDFAVTFLDRYGNEIGQRGIIQRDSVPVDEMPDHVIKAVLATEDRRFFDHYGIDVLGLSRAIFENV
RANSVVQGGSSITQQLAKNLFLTNERTFERKIKEAFLSLWLEANLSKKEILQLYLDRAYMGGGTFGIEAAADFYFGKSVK
DLNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVLSNLVDSGFMTEGQVLQARLHPADVVDRGEQKSPDYFLDWAFDE
VKKIAKPGQHSLVAHTTFDANIQKAAEESVEFHLRQFGKEYNVTEGAVVVIETNGAVRAIVGGRDYGASQFNRATKALRQ
TGSSFKPYVYATAMEHGFTPNSIISGGPISWGNWSPHNYSGESAGNITLIMAMAKSINTVPVRLAKDHLGIAPIKAMAEA
MGVESPLESHKTMVLGTSGMTVMDQATGYSVFAQNGFVGSRHGITQLVTRTGDVVYDWTKDATPPHRVLSEQALKSMNTM
LAAVPVMGTARRAQIPNIVVAGKTGTTQSYRDAWFVGFTGNYTAAVWLGNDDFTPTKNMTGGSLPAMVWQRLMVYAHQNI
DLKPIPGIDKPFVDEDIAAKAAEAQKKSEDEAAADAAAERPAVLSSRTTQTLRDMTQLFQSAPKLDAAASPETLSAL

Sequences:

>Translated_717_residues
MVSPEPRKRRGPIASRLLALDAWIDSSLYEIGFKARQFWEAATIFSRRFRLKGWRRGIIEILSETFTLGAGGIVVMLALA
IPAFQDTAGDWRAQGDFAVTFLDRYGNEIGQRGIIQRDSVPVDEMPDHVIKAVLATEDRRFFDHYGIDVLGLSRAIFENV
RANSVVQGGSSITQQLAKNLFLTNERTFERKIKEAFLSLWLEANLSKKEILQLYLDRAYMGGGTFGIEAAADFYFGKSVK
DLNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVLSNLVDSGFMTEGQVLQARLHPADVVDRGEQKSPDYFLDWAFDE
VKKIAKPGQHSLVAHTTFDANIQKAAEESVEFHLRQFGKEYNVTEGAVVVIETNGAVRAIVGGRDYGASQFNRATKALRQ
TGSSFKPYVYATAMEHGFTPNSIISGGPISWGNWSPHNYSGESAGNITLIMAMAKSINTVPVRLAKDHLGIAPIKAMAEA
MGVESPLESHKTMVLGTSGMTVMDQATGYSVFAQNGFVGSRHGITQLVTRTGDVVYDWTKDATPPHRVLSEQALKSMNTM
LAAVPVMGTARRAQIPNIVVAGKTGTTQSYRDAWFVGFTGNYTAAVWLGNDDFTPTKNMTGGSLPAMVWQRLMVYAHQNI
DLKPIPGIDKPFVDEDIAAKAAEAQKKSEDEAAADAAAERPAVLSSRTTQTLRDMTQLFQSAPKLDAAASPETLSAL
>Mature_717_residues
MVSPEPRKRRGPIASRLLALDAWIDSSLYEIGFKARQFWEAATIFSRRFRLKGWRRGIIEILSETFTLGAGGIVVMLALA
IPAFQDTAGDWRAQGDFAVTFLDRYGNEIGQRGIIQRDSVPVDEMPDHVIKAVLATEDRRFFDHYGIDVLGLSRAIFENV
RANSVVQGGSSITQQLAKNLFLTNERTFERKIKEAFLSLWLEANLSKKEILQLYLDRAYMGGGTFGIEAAADFYFGKSVK
DLNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVLSNLVDSGFMTEGQVLQARLHPADVVDRGEQKSPDYFLDWAFDE
VKKIAKPGQHSLVAHTTFDANIQKAAEESVEFHLRQFGKEYNVTEGAVVVIETNGAVRAIVGGRDYGASQFNRATKALRQ
TGSSFKPYVYATAMEHGFTPNSIISGGPISWGNWSPHNYSGESAGNITLIMAMAKSINTVPVRLAKDHLGIAPIKAMAEA
MGVESPLESHKTMVLGTSGMTVMDQATGYSVFAQNGFVGSRHGITQLVTRTGDVVYDWTKDATPPHRVLSEQALKSMNTM
LAAVPVMGTARRAQIPNIVVAGKTGTTQSYRDAWFVGFTGNYTAAVWLGNDDFTPTKNMTGGSLPAMVWQRLMVYAHQNI
DLKPIPGIDKPFVDEDIAAKAAEAQKKSEDEAAADAAAERPAVLSSRTTQTLRDMTQLFQSAPKLDAAASPETLSAL

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=571, Percent_Identity=32.5744308231173, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788867, Length=520, Percent_Identity=29.4230769230769, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87082258, Length=230, Percent_Identity=43.9130434782609, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=37.7049180327869, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 78372; Mature: 78372

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPEPRKRRGPIASRLLALDAWIDSSLYEIGFKARQFWEAATIFSRRFRLKGWRRGIIE
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSETFTLGAGGIVVMLALAIPAFQDTAGDWRAQGDFAVTFLDRYGNEIGQRGIIQRDSV
HHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCC
PVDEMPDHVIKAVLATEDRRFFDHYGIDVLGLSRAIFENVRANSVVQGGSSITQQLAKNL
CHHHCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FLTNERTFERKIKEAFLSLWLEANLSKKEILQLYLDRAYMGGGTFGIEAAADFYFGKSVK
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
DLNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVLSNLVDSGFMTEGQVLQARLHPAD
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHH
VVDRGEQKSPDYFLDWAFDEVKKIAKPGQHSLVAHTTFDANIQKAAEESVEFHLRQFGKE
HHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
YNVTEGAVVVIETNGAVRAIVGGRDYGASQFNRATKALRQTGSSFKPYVYATAMEHGFTP
CCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCC
NSIISGGPISWGNWSPHNYSGESAGNITLIMAMAKSINTVPVRLAKDHLGIAPIKAMAEA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHH
MGVESPLESHKTMVLGTSGMTVMDQATGYSVFAQNGFVGSRHGITQLVTRTGDVVYDWTK
HCCCCHHHHCCEEEEECCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEECCC
DATPPHRVLSEQALKSMNTMLAAVPVMGTARRAQIPNIVVAGKTGTTQSYRDAWFVGFTG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECC
NYTAAVWLGNDDFTPTKNMTGGSLPAMVWQRLMVYAHQNIDLKPIPGIDKPFVDEDIAAK
CEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AAEAQKKSEDEAAADAAAERPAVLSSRTTQTLRDMTQLFQSAPKLDAAASPETLSAL
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MVSPEPRKRRGPIASRLLALDAWIDSSLYEIGFKARQFWEAATIFSRRFRLKGWRRGIIE
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSETFTLGAGGIVVMLALAIPAFQDTAGDWRAQGDFAVTFLDRYGNEIGQRGIIQRDSV
HHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCC
PVDEMPDHVIKAVLATEDRRFFDHYGIDVLGLSRAIFENVRANSVVQGGSSITQQLAKNL
CHHHCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FLTNERTFERKIKEAFLSLWLEANLSKKEILQLYLDRAYMGGGTFGIEAAADFYFGKSVK
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
DLNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVLSNLVDSGFMTEGQVLQARLHPAD
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHH
VVDRGEQKSPDYFLDWAFDEVKKIAKPGQHSLVAHTTFDANIQKAAEESVEFHLRQFGKE
HHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
YNVTEGAVVVIETNGAVRAIVGGRDYGASQFNRATKALRQTGSSFKPYVYATAMEHGFTP
CCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCC
NSIISGGPISWGNWSPHNYSGESAGNITLIMAMAKSINTVPVRLAKDHLGIAPIKAMAEA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHH
MGVESPLESHKTMVLGTSGMTVMDQATGYSVFAQNGFVGSRHGITQLVTRTGDVVYDWTK
HCCCCHHHHCCEEEEECCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHCCCEEEECCC
DATPPHRVLSEQALKSMNTMLAAVPVMGTARRAQIPNIVVAGKTGTTQSYRDAWFVGFTG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECC
NYTAAVWLGNDDFTPTKNMTGGSLPAMVWQRLMVYAHQNIDLKPIPGIDKPFVDEDIAAK
CEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AAEAQKKSEDEAAADAAAERPAVLSSRTTQTLRDMTQLFQSAPKLDAAASPETLSAL
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]