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The map label for this gene is glgX [H]

Identifier: 13476307

GI number: 13476307

Start: 6280075

End: 6282093

Strand: Direct

Name: glgX [H]

Synonym: mlr7591

Alternate gene names: 13476307

Gene position: 6280075-6282093 (Clockwise)

Preceding gene: 13476306

Following gene: 13476308

Centisome position: 89.26

GC content: 66.12

Gene sequence:

>2019_bases
ATGACCCCGCTCGGCGCAACCGTCACCCCCGAAGGCATCCGCTTTGCCGTGTGGTCCTCATCGGCCCGCCGCCTCTGGGT
CTCGCTCTTCGATGGGCAGGGAAACCGCGAACTCGACCGGTTGGAGTTGCAGTCGGAAGGCGAGGGGATGCATGCGCTCT
TCGTTCCCCGCCTTGCCGCGGGCACGCGGTATGGCTTTCGCGCCGATGGCGACTACGCGCCCGACCGCGGGTTGTGGTTC
GACCCGGACAAGCTGCTCACCGATCCCTACGCCGTCGAAATCGACCGCCCGTATGCCTATCACTGGCGGCTCGCCGCGCG
GCGCAATGAGGGGGCCGACACCGCGCCGCTGATGCCCAAGACGGTGGCGAAAACCTTGCCCAAAACGGTGCCTGCGCTGC
CGCCGCTGTTTCGTCCGGGCGGCCTGATCTACGAGGTACCGGTGCGTGCCTTCACCAAGTTGCATCCCGACATTCCCGAA
GCGCAGCGCGGTACGATCGCGGCGCTTGCTCATCCTGTCATCATCGAACATCTGCAAAGGCTCGGCGTGTCGGCCGTCGA
ACTGATGCCGGTGACGGCGTCGATCGACGAACGGCACCTGCCGCCGCTGGGCCTCAGCAACGCCTGGTGCTACAATCCCG
TCACCTTCATGGCGCTCGACCCGCGTCTCGCGCCCGGCGGCCTCGAGGAATTGCGCGACACCGTGGCGGCGCTGCGCAAG
GCCAGCATCGGCACCATCCTCGACCTCGTCTTCAACCACACGGGCGAAAGCGACCGGCTGGGGCCGACGCTGTCGCTGCG
CGGGCTCGACAACCAGGCCTACTACCGGCACCAGCCTGATGGCAGGCTGGTCAATGACACCGGCACCGGCAACACAGTCG
CCTGCGATCATCCGGTGGTGCGGGACATGGTGCTCGACACGCTCCGTCACTTCGTCCGCCATACCGGCGTCGATGGCTTC
CGCTTCGATCTGGCGCCGGTTCTGGGCCGCGTCGATGGCGCATTCGATCCCACGGCGCCCTTGCTCGAGGCGATCACCGG
CGATCCTGTGCTTGCCGATCGCGTGCTGATCGCGGAGCCGTGGGATATCGGCCCTGACGGCTATCAGCTCGGCAACTTCC
CGCCGCGCTTCCTCGAATGGAACGATCGCTATCGCGACGATGCCAGGCGCTTCTGGCGCGGCGATGCCGGTGCGGTGGGA
GCACTGGCGACGCGGCTTGCCGGTTCGTCCGATATTTTTGGCAAGGCCGGCCAGGCGGCCAGCCGCACGGTCAATTTCAT
CGCTGCCCATGACGGCATGACTTTGGCCGACATCGTCGCCTATGAGCGCAAGCACAACGAGGCCAATGGCGAGCAGAACC
GCGACGGTCACAACGACAATCTGTCCTGGAACAATGGCGCCGAGGGCGAGACGGATAGCGCGGCGATCGCCAATGCGCGC
TTCGACGACCAGTGCGCGCTGCTCGCCACGCTTTTTGCCTCGCGCGGCACCATCATGCTGACGGCGGGCGACGAGTTCGG
CCGCTCCCAGAAGGGCAACAACAACGCCTATGCGCAGGACAATGCCATCACCTGGCTCGACTGGGCCGGCCGCGACCATG
CGCTGGAGCGCTACGCATCGGTGCTCGGCATGCTGCGCCGTGCCGTCCCGGCCCTGTCGGCTACAGATTTCCTCACAGGA
CAATCGGCCGATGCGACAGGCACCCCCGACGTCGCCTGGTTGACCGAGACCGGCATGCCGCTTGCCGAGACGGACTGGAA
CGACCCCGCCCGGCACCGCCTCGTCATGCTGCTTGGCGATGCAGGCGGCGATCGCCTTGCCGTCATGATCAACGGCGATC
GTCGCCAATGCGTCTTCACCTTGCCCGAACGGGAAGGCTTCCGATGGCAGCCGGCGATCGAGACGCAGGCGATTGAGCTT
GCGCGGCCGCTGCCGGGCCGAAGCGTCCATTTCATGGCAGAGCATCGGACCGAAAAGTGGCAGTCGGTTTTCGGGCAATC
CGATGCGAGCAAAAAATAG

Upstream 100 bases:

>100_bases
CCAGACCACCGGCGCCTCATTCCTTCCAACACCCCTCATCCGTCTCGGCGCTACGCGCCGATCCACCTTCTCCCACAAGG
GGAGAAGGGAAGGCGCTGAC

Downstream 100 bases:

>100_bases
TTCGCGCCAGGCCGCAAAGGGCTGCGCCAGGAGGGGTTCGTGATGGCCGACGACAATGCGGGGCAGGGGGCCGACTGGTG
GCGCGGCTGCGTCATCTACC

Product: glycosyl hydrolase

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTPLGATVTPEGIRFAVWSSSARRLWVSLFDGQGNRELDRLELQSEGEGMHALFVPRLAAGTRYGFRADGDYAPDRGLWF
DPDKLLTDPYAVEIDRPYAYHWRLAARRNEGADTAPLMPKTVAKTLPKTVPALPPLFRPGGLIYEVPVRAFTKLHPDIPE
AQRGTIAALAHPVIIEHLQRLGVSAVELMPVTASIDERHLPPLGLSNAWCYNPVTFMALDPRLAPGGLEELRDTVAALRK
ASIGTILDLVFNHTGESDRLGPTLSLRGLDNQAYYRHQPDGRLVNDTGTGNTVACDHPVVRDMVLDTLRHFVRHTGVDGF
RFDLAPVLGRVDGAFDPTAPLLEAITGDPVLADRVLIAEPWDIGPDGYQLGNFPPRFLEWNDRYRDDARRFWRGDAGAVG
ALATRLAGSSDIFGKAGQAASRTVNFIAAHDGMTLADIVAYERKHNEANGEQNRDGHNDNLSWNNGAEGETDSAAIANAR
FDDQCALLATLFASRGTIMLTAGDEFGRSQKGNNNAYAQDNAITWLDWAGRDHALERYASVLGMLRRAVPALSATDFLTG
QSADATGTPDVAWLTETGMPLAETDWNDPARHRLVMLLGDAGGDRLAVMINGDRRQCVFTLPEREGFRWQPAIETQAIEL
ARPLPGRSVHFMAEHRTEKWQSVFGQSDASKK

Sequences:

>Translated_672_residues
MTPLGATVTPEGIRFAVWSSSARRLWVSLFDGQGNRELDRLELQSEGEGMHALFVPRLAAGTRYGFRADGDYAPDRGLWF
DPDKLLTDPYAVEIDRPYAYHWRLAARRNEGADTAPLMPKTVAKTLPKTVPALPPLFRPGGLIYEVPVRAFTKLHPDIPE
AQRGTIAALAHPVIIEHLQRLGVSAVELMPVTASIDERHLPPLGLSNAWCYNPVTFMALDPRLAPGGLEELRDTVAALRK
ASIGTILDLVFNHTGESDRLGPTLSLRGLDNQAYYRHQPDGRLVNDTGTGNTVACDHPVVRDMVLDTLRHFVRHTGVDGF
RFDLAPVLGRVDGAFDPTAPLLEAITGDPVLADRVLIAEPWDIGPDGYQLGNFPPRFLEWNDRYRDDARRFWRGDAGAVG
ALATRLAGSSDIFGKAGQAASRTVNFIAAHDGMTLADIVAYERKHNEANGEQNRDGHNDNLSWNNGAEGETDSAAIANAR
FDDQCALLATLFASRGTIMLTAGDEFGRSQKGNNNAYAQDNAITWLDWAGRDHALERYASVLGMLRRAVPALSATDFLTG
QSADATGTPDVAWLTETGMPLAETDWNDPARHRLVMLLGDAGGDRLAVMINGDRRQCVFTLPEREGFRWQPAIETQAIEL
ARPLPGRSVHFMAEHRTEKWQSVFGQSDASKK
>Mature_671_residues
TPLGATVTPEGIRFAVWSSSARRLWVSLFDGQGNRELDRLELQSEGEGMHALFVPRLAAGTRYGFRADGDYAPDRGLWFD
PDKLLTDPYAVEIDRPYAYHWRLAARRNEGADTAPLMPKTVAKTLPKTVPALPPLFRPGGLIYEVPVRAFTKLHPDIPEA
QRGTIAALAHPVIIEHLQRLGVSAVELMPVTASIDERHLPPLGLSNAWCYNPVTFMALDPRLAPGGLEELRDTVAALRKA
SIGTILDLVFNHTGESDRLGPTLSLRGLDNQAYYRHQPDGRLVNDTGTGNTVACDHPVVRDMVLDTLRHFVRHTGVDGFR
FDLAPVLGRVDGAFDPTAPLLEAITGDPVLADRVLIAEPWDIGPDGYQLGNFPPRFLEWNDRYRDDARRFWRGDAGAVGA
LATRLAGSSDIFGKAGQAASRTVNFIAAHDGMTLADIVAYERKHNEANGEQNRDGHNDNLSWNNGAEGETDSAAIANARF
DDQCALLATLFASRGTIMLTAGDEFGRSQKGNNNAYAQDNAITWLDWAGRDHALERYASVLGMLRRAVPALSATDFLTGQ
SADATGTPDVAWLTETGMPLAETDWNDPARHRLVMLLGDAGGDRLAVMINGDRRQCVFTLPEREGFRWQPAIETQAIELA
RPLPGRSVHFMAEHRTEKWQSVFGQSDASKK

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=630, Percent_Identity=43.4920634920635, Blast_Score=470, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73814; Mature: 73683

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLGATVTPEGIRFAVWSSSARRLWVSLFDGQGNRELDRLELQSEGEGMHALFVPRLAA
CCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEEEECHHHC
GTRYGFRADGDYAPDRGLWFDPDKLLTDPYAVEIDRPYAYHWRLAARRNEGADTAPLMPK
CCCCCCCCCCCCCCCCCCEECCHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHH
TVAKTLPKTVPALPPLFRPGGLIYEVPVRAFTKLHPDIPEAQRGTIAALAHPVIIEHLQR
HHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVSAVELMPVTASIDERHLPPLGLSNAWCYNPVTFMALDPRLAPGGLEELRDTVAALRK
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ASIGTILDLVFNHTGESDRLGPTLSLRGLDNQAYYRHQPDGRLVNDTGTGNTVACDHPVV
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEECCCCCCEEECCCHHH
RDMVLDTLRHFVRHTGVDGFRFDLAPVLGRVDGAFDPTAPLLEAITGDPVLADRVLIAEP
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCHHCCEEEEECC
WDIGPDGYQLGNFPPRFLEWNDRYRDDARRFWRGDAGAVGALATRLAGSSDIFGKAGQAA
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHH
SRTVNFIAAHDGMTLADIVAYERKHNEANGEQNRDGHNDNLSWNNGAEGETDSAAIANAR
HHEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
FDDQCALLATLFASRGTIMLTAGDEFGRSQKGNNNAYAQDNAITWLDWAGRDHALERYAS
CCCHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHH
VLGMLRRAVPALSATDFLTGQSADATGTPDVAWLTETGMPLAETDWNDPARHRLVMLLGD
HHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEC
AGGDRLAVMINGDRRQCVFTLPEREGFRWQPAIETQAIELARPLPGRSVHFMAEHRTEKW
CCCCEEEEEEECCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCCCEEEHHHHHHHHH
QSVFGQSDASKK
HHHHCCCCCCCC
>Mature Secondary Structure 
TPLGATVTPEGIRFAVWSSSARRLWVSLFDGQGNRELDRLELQSEGEGMHALFVPRLAA
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEEEECHHHC
GTRYGFRADGDYAPDRGLWFDPDKLLTDPYAVEIDRPYAYHWRLAARRNEGADTAPLMPK
CCCCCCCCCCCCCCCCCCEECCHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHH
TVAKTLPKTVPALPPLFRPGGLIYEVPVRAFTKLHPDIPEAQRGTIAALAHPVIIEHLQR
HHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVSAVELMPVTASIDERHLPPLGLSNAWCYNPVTFMALDPRLAPGGLEELRDTVAALRK
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ASIGTILDLVFNHTGESDRLGPTLSLRGLDNQAYYRHQPDGRLVNDTGTGNTVACDHPVV
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEECCCCCCEEECCCHHH
RDMVLDTLRHFVRHTGVDGFRFDLAPVLGRVDGAFDPTAPLLEAITGDPVLADRVLIAEP
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCHHCCEEEEECC
WDIGPDGYQLGNFPPRFLEWNDRYRDDARRFWRGDAGAVGALATRLAGSSDIFGKAGQAA
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHH
SRTVNFIAAHDGMTLADIVAYERKHNEANGEQNRDGHNDNLSWNNGAEGETDSAAIANAR
HHEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
FDDQCALLATLFASRGTIMLTAGDEFGRSQKGNNNAYAQDNAITWLDWAGRDHALERYAS
CCCHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHH
VLGMLRRAVPALSATDFLTGQSADATGTPDVAWLTETGMPLAETDWNDPARHRLVMLLGD
HHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEC
AGGDRLAVMINGDRRQCVFTLPEREGFRWQPAIETQAIELARPLPGRSVHFMAEHRTEKW
CCCCEEEEEEECCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCCCEEEHHHHHHHHH
QSVFGQSDASKK
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA