Click here to switch to the map view.

The map label for this gene is glgB

Identifier: 13476303

GI number: 13476303

Start: 6273182

End: 6275395

Strand: Direct

Name: glgB

Synonym: mlr7587

Alternate gene names: 13476303

Gene position: 6273182-6275395 (Clockwise)

Preceding gene: 13476302

Following gene: 13476304

Centisome position: 89.16

GC content: 62.69

Gene sequence:

>2214_bases
ATGAGGAAGCCGCGCGTGACCGCTGCGACAAGCGGGCCGGACGGACTGGCGCCAGCCAGCGATGTTGCGGCGATTGTCGC
CGGTACGCACGGAGATCCGTTCGCGGTCCTCGGTGTCCATGAGGTCGGCAAAGGCCTGTTTGCCCGTTGTTTCGTTCCGC
ATGCCGAGACTGTCACTGCCTATACGCTGACGGGCATCGAGGCCGGTGCGTTGTCCAGGCGAGACGATGCTGGCTTCTTC
GAAGGCAAGCTGTCGATCAAGAAGCGGCAGCCGCTGCGCTATCACGCGCACAATGCCGGCGGCGACTGGTGGCTGACCGA
TCCTTATTCGTTCGGCCCGGTGCTCGGGCCGATGGACGATTACTATATCGCCGAAGGTTCGCATCTCAGGCTGTTCGACA
AGCTTGGCGCGCATGTCATCGAGCACGAAGGCGCCACCGGCGTGCATTTCGCCGTCTGGGCTCCCAACGCAAAACGCGTC
TCGGTGGTTGGCGATTTCAACGACTGGGATGGCCGCCGCCACACGATGCGCGATCGCCGCGACACCGGCATCTGGGAGGT
GTTCATCCCCGACATCGGTGCCGGGCGACCCTACAAATACGAGATCATCGGACCCGATGGCGTCAGGCTGCCGCTGAAGG
CCGATCCGTTTGCGTTCAAATCCGAATTGCGTCCTGCCACGGCTTCAGTGGTCGCCGTGCCGCCGGCGCATGACTGGGGC
GACGAGGCGCATCGCAACTACTGGCGCAATGCCGATCCCCGGCGCGAAGCCGTCTCGATCTACGAAGTCCATGCCGGGTC
ATGGCAACTTCACGACGACGGCACGTTCCTGTCATGGGACGAGCTGGCCGACCGGCTGATCCCCTATGTCGTCGAGACCG
GCTTCACCCACATCGAGTTCATGCCGATCTCCGAGCATCCCTATGATCCGTCCTGGGGCTATCAGACCACCGGCCTCTAT
GCGCCGTCGGCCCGCTTCGGCGATCCTGACGGCTTTGCCCGTTTCGTTGATGGCGCGCACCGCGCCGGCGTCGGCGTCAT
CCTCGATTGGGTGCCGGCGCATTTCCCGGTCGATGCGCACGGCCTGGCCCATTTCGACGGCACCGCGCTCTACGAACATG
CCGATCCGCGCAAGGGTTTTCACCCCGACTGGAACACCGCGATCTACAATTTCGGGCGCCGCGAGGTGGTCTCGTTCCTG
GTCAACAATGCGCTGTTCTGGGCCGAAAAATATCATGTCGACGGTTTGCGCGTCGATGCGGTCGCCTCGATGCTCTACCT
CGACTATTCGCGCAAGGCCGGCGAGTGGATCCCCAACGAGAAGGGCGGCCGCGAGAACCTCGAGGCGGTCTCTTTCCTGC
AGAAGATGAACAAGGAGGTCTACGGCCACCATCCAGGCGTCATGACCATCGCCGAGGAATCGACCTCCTGGCCGAAGGTT
TCGGCGCCGGTGCACGAAGGCGGGCTGGGCTTCGGCTTCAAGTGGAACATGGGCTTCATGCATGACACGCTGGAGTATTT
CTCCAAGGAGCCGATTTTCCGCAAGCACCACCACAACGACCTGACCTTCGGCCTGACCTACGCCTTCTCGGAGAATTTCG
TGCTGCCGCTCTCGCATGACGAGGTCGTGCACGGCAAAGGCACGCTGCTGAGCAAGATGGCCGGCGACGACTGGCAGAAA
TTCGCAACCTTGCGCGCCTATTACGGCTTCATGTGGGGCTATCCCGGCAAGAAGCTCTTGTTCATGGGACAGGAATTCGC
GCAGCGCCGTGAATGGAGCGAGGCGCGTGCTCTTGACTGGAACCTGCTCGATTTCCGCCCGCATCGCGGCGTCTGGCAGA
CGGTGCGCGATCTCAACTATCTCTACCGCTCGCGCCCGGCGCTGCACGGCCGCGACTGCGAGCCGGAAGGATTTTCCTGG
CTGATCGTCGATGACAGCCAGAATTCAGTTTTCGCCTGGGTGCGCAACGCGCCGGGCGGCAGCCCGGTGGCCGTTATCTC
CAACTTCACGCCGGTGCCACGCGACAATTATCGCGTGCCGCTGCCGAAGGCTGGCAAGTGGCGCGAGATCATCAACACCG
ACGCATCCGAATATGGCGGCTCCGGCATGGGCAATGGCGGCATGGTCGAGGCAAGGGCGGAAGGCAAGAACATCTCTGCA
ACGATGCTTCTGCCGCCGCTGTCGACAATCATGCTCGAACTCGTTGCCGACTGA

Upstream 100 bases:

>100_bases
TGCATGAGGCCACGAACAATGTGGTCCCGGTGACGTGGGTTGAATGCCAACGCGCATCACCTGAAATAGACACCTGGTGC
CCTGGGAGGGACACTGCCTG

Downstream 100 bases:

>100_bases
GCCCGTCAAGGCCGAACAGCAGGGCGAGACTTGAGTAACTTGGGAGGGTAGAAATGGCAGACTTGAAACGGACCCAGCCG
CTGGCGCGCGATGCCATGGC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTAYTLTGIEAGALSRRDDAGFF
EGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDDYYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRV
SVVGDFNDWDGRRHTMRDRRDTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG
DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEFMPISEHPYDPSWGYQTTGLY
APSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAHGLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFL
VNNALFWAEKYHVDGLRVDAVASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV
SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHDEVVHGKGTLLSKMAGDDWQK
FATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDWNLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSW
LIVDDSQNSVFAWVRNAPGGSPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA
TMLLPPLSTIMLELVAD

Sequences:

>Translated_737_residues
MRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTAYTLTGIEAGALSRRDDAGFF
EGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDDYYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRV
SVVGDFNDWDGRRHTMRDRRDTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG
DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEFMPISEHPYDPSWGYQTTGLY
APSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAHGLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFL
VNNALFWAEKYHVDGLRVDAVASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV
SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHDEVVHGKGTLLSKMAGDDWQK
FATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDWNLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSW
LIVDDSQNSVFAWVRNAPGGSPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA
TMLLPPLSTIMLELVAD
>Mature_737_residues
MRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTAYTLTGIEAGALSRRDDAGFF
EGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDDYYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRV
SVVGDFNDWDGRRHTMRDRRDTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG
DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEFMPISEHPYDPSWGYQTTGLY
APSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAHGLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFL
VNNALFWAEKYHVDGLRVDAVASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV
SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHDEVVHGKGTLLSKMAGDDWQK
FATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDWNLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSW
LIVDDSQNSVFAWVRNAPGGSPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA
TMLLPPLSTIMLELVAD

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=602, Percent_Identity=27.0764119601329, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1789839, Length=720, Percent_Identity=53.75, Blast_Score=764, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=381, Percent_Identity=28.3464566929134, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32564391, Length=269, Percent_Identity=31.2267657992565, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320826, Length=380, Percent_Identity=29.2105263157895, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI28573410, Length=604, Percent_Identity=28.6423841059603, Blast_Score=183, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_RHILO (Q985P4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107873.1
- ProteinModelPortal:   Q985P4
- SMR:   Q985P4
- GeneID:   1230528
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7587
- NMPDR:   fig|266835.1.peg.5974
- HOGENOM:   HBG287139
- OMA:   INTDASE
- ProtClustDB:   PRK05402
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 82526; Mature: 82526

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: ACT_SITE 314-314 ACT_SITE 349-349 ACT_SITE 354-354 ACT_SITE 417-417 ACT_SITE 419-419 ACT_SITE 472-472 ACT_SITE 539-539 ACT_SITE 540-540

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTA
CCCCCEEEECCCCCCCCCHHHCEEEEECCCCCCEEEEEHHHHCCCCEEEEECCCCCEEEE
YTLTGIEAGALSRRDDAGFFEGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDD
EEEECCCCCCCCCCCCCCEEECEEEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCC
YYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRVSVVGDFNDWDGRRHTMRDRR
EEEECCCCEEHHHHHCCCEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCC
DTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG
CCCEEEEEECCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHCCCCCEEEEECCCCCCCC
DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEF
CHHHHHHCCCCCCHHHEEEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEE
MPISEHPYDPSWGYQTTGLYAPSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAH
EECCCCCCCCCCCCEECCEECCCCCCCCCCHHHHHHCCHHHCCCEEEEECCCCCCCCCCC
GLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFLVNNALFWAEKYHVDGLRVDA
CEEECCCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHEEEEEEECCCEEHHH
VASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHD
CCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCEEEECCCC
EVVHGKGTLLSKMAGDDWQKFATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDW
CEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCC
NLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSWLIVDDSQNSVFAWVRNAPGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
SPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA
CCEEEEECCCCCCCCCCEECCCCCCCHHHHHCCCHHHHCCCCCCCCCEEEEECCCCCEEE
TMLLPPLSTIMLELVAD
EEECCCHHHHHHHHHCC
>Mature Secondary Structure
MRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTA
CCCCCEEEECCCCCCCCCHHHCEEEEECCCCCCEEEEEHHHHCCCCEEEEECCCCCEEEE
YTLTGIEAGALSRRDDAGFFEGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDD
EEEECCCCCCCCCCCCCCEEECEEEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCC
YYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRVSVVGDFNDWDGRRHTMRDRR
EEEECCCCEEHHHHHCCCEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCC
DTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG
CCCEEEEEECCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHCCCCCEEEEECCCCCCCC
DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEF
CHHHHHHCCCCCCHHHEEEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEE
MPISEHPYDPSWGYQTTGLYAPSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAH
EECCCCCCCCCCCCEECCEECCCCCCCCCCHHHHHHCCHHHCCCEEEEECCCCCCCCCCC
GLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFLVNNALFWAEKYHVDGLRVDA
CEEECCCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHEEEEEEECCCEEHHH
VASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHD
CCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCEEEECCCC
EVVHGKGTLLSKMAGDDWQKFATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDW
CEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCC
NLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSWLIVDDSQNSVFAWVRNAPGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
SPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA
CCEEEEECCCCCCCCCCEECCCCCCCHHHHHCCCHHHHCCCCCCCCCEEEEECCCCCEEE
TMLLPPLSTIMLELVAD
EEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968