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The map label for this gene is paaF [C]

Identifier: 13476196

GI number: 13476196

Start: 6158987

End: 6159745

Strand: Direct

Name: paaF [C]

Synonym: mlr7453

Alternate gene names: 13476196

Gene position: 6158987-6159745 (Clockwise)

Preceding gene: 13476195

Following gene: 13476198

Centisome position: 87.53

GC content: 66.4

Gene sequence:

>759_bases
ATGACCGTGACAGACCATATCCTCGTCGAGCGCCAGGGCGCCATCCAGATCATCCGCATGAACCGCCCGGACAAGAAGAA
CGCGCTGACGCGTGCCATGTACGCGAAAATGTCGGCGGCGCTTGCCGAAGGCGATGCCGATCCGGGGATCCGCGTCCACG
TCTTCCTAGGCGTGCCAGGCGCCTTCTCCTCCGGCAACGACCTTGCCGATTTCATGGTTGTCGCCAGCGGCGGTGACGGC
GGCACGGAGGTCTGGGATTTCCTGATGGCGCTGGCCAGGGTCGAAAAAGCCATCGTCTCCGGCGTCGACGGCATCGCGGT
CGGCATCGGCACGACGCTCAACCTGCATTGCGACCTGACCTTCGCCACGCCGCGCACCGTGTTCAGGACGCCTTTCGTCG
ATCTTGGCCTGGTGCCCGAGGCCGGCTCCAGTCTGCTGGCGCCGCGCATCCTGGGTCAGCAGGGCGCTTTTGCGCTGCTC
GGCCTTGGCGAAGGTTTTTCCGCCGAGCGCGCGAAGGCCGCCGGCCTGATCTACGATGTGGTCGAGGAGGCCGCGCTGGA
GGCCTCGGTCCTTGCAGCCGCCAGCCAGATCGCCGCCAAGCCGCCGCAGGCGCTGAAAATCGCGCGCGATCTCATGCGCG
GCTCGCGTGACGATCTCGTCGCCCGCATCGGCGAGGAAAGCGAACATTTTCGCGAGCGGCTGAAGTCGGATGAGGCCCGC
GCGGCGCTCACCGCGTTCATGACCAGGAAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CGCGCCTTGAGGGAACGCGTCATCGACGGCGCGGAGAGCCTCGCTGCCGCCGGCAAGACGTTGATATCAGCTTGAATTCT
CACAGCGCTCACCAGGGAAG

Downstream 100 bases:

>100_bases
GGCCTTGCCCTTTCAGGGATAGAGCCGCGCCTTGTCCCAATCGCCTTTCGCGTTGCGGGAAAAAACCACCCGGTCATGCA
GCCGGAATGGGCGGTCGTGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTVTDHILVERQGAIQIIRMNRPDKKNALTRAMYAKMSAALAEGDADPGIRVHVFLGVPGAFSSGNDLADFMVVASGGDG
GTEVWDFLMALARVEKAIVSGVDGIAVGIGTTLNLHCDLTFATPRTVFRTPFVDLGLVPEAGSSLLAPRILGQQGAFALL
GLGEGFSAERAKAAGLIYDVVEEAALEASVLAAASQIAAKPPQALKIARDLMRGSRDDLVARIGEESEHFRERLKSDEAR
AALTAFMTRKKG

Sequences:

>Translated_252_residues
MTVTDHILVERQGAIQIIRMNRPDKKNALTRAMYAKMSAALAEGDADPGIRVHVFLGVPGAFSSGNDLADFMVVASGGDG
GTEVWDFLMALARVEKAIVSGVDGIAVGIGTTLNLHCDLTFATPRTVFRTPFVDLGLVPEAGSSLLAPRILGQQGAFALL
GLGEGFSAERAKAAGLIYDVVEEAALEASVLAAASQIAAKPPQALKIARDLMRGSRDDLVARIGEESEHFRERLKSDEAR
AALTAFMTRKKG
>Mature_251_residues
TVTDHILVERQGAIQIIRMNRPDKKNALTRAMYAKMSAALAEGDADPGIRVHVFLGVPGAFSSGNDLADFMVVASGGDGG
TEVWDFLMALARVEKAIVSGVDGIAVGIGTTLNLHCDLTFATPRTVFRTPFVDLGLVPEAGSSLLAPRILGQQGAFALLG
LGEGFSAERAKAAGLIYDVVEEAALEASVLAAASQIAAKPPQALKIARDLMRGSRDDLVARIGEESEHFRERLKSDEARA
ALTAFMTRKKG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=253, Percent_Identity=32.4110671936759, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI260275230, Length=253, Percent_Identity=32.4110671936759, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI260274832, Length=253, Percent_Identity=32.4110671936759, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=24.3027888446215, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI25453481, Length=220, Percent_Identity=26.3636363636364, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538573, Length=220, Percent_Identity=26.3636363636364, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4757966, Length=220, Percent_Identity=26.3636363636364, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI51538581, Length=220, Percent_Identity=26.3636363636364, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4757968, Length=222, Percent_Identity=26.1261261261261, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI49355787, Length=222, Percent_Identity=26.1261261261261, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=29.8913043478261, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI221136756, Length=254, Percent_Identity=22.0472440944882, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=254, Percent_Identity=22.0472440944882, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI116235487, Length=229, Percent_Identity=24.0174672489083, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594471, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=22.9249011857708, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI70995211, Length=226, Percent_Identity=25.6637168141593, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=274, Percent_Identity=26.2773722627737, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=28.2352941176471, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=26.3940520446097, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788597, Length=205, Percent_Identity=28.2926829268293, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=31.2741312741313, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=32.2448979591837, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=26.984126984127, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=24.7148288973384, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=28.9617486338798, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=224, Percent_Identity=28.125, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=28.9617486338798, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=219, Percent_Identity=26.4840182648402, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=28.0788177339901, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=255, Percent_Identity=30.5882352941176, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129971, Length=238, Percent_Identity=24.3697478991597, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653477, Length=238, Percent_Identity=24.3697478991597, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=27.7992277992278, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21357171, Length=226, Percent_Identity=26.9911504424779, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26662; Mature: 26531

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTDHILVERQGAIQIIRMNRPDKKNALTRAMYAKMSAALAEGDADPGIRVHVFLGVPG
CCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
AFSSGNDLADFMVVASGGDGGTEVWDFLMALARVEKAIVSGVDGIAVGIGTTLNLHCDLT
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEE
FATPRTVFRTPFVDLGLVPEAGSSLLAPRILGQQGAFALLGLGEGFSAERAKAAGLIYDV
ECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
VEEAALEASVLAAASQIAAKPPQALKIARDLMRGSRDDLVARIGEESEHFRERLKSDEAR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCHHH
AALTAFMTRKKG
HHHHHHHHHCCC
>Mature Secondary Structure 
TVTDHILVERQGAIQIIRMNRPDKKNALTRAMYAKMSAALAEGDADPGIRVHVFLGVPG
CCCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
AFSSGNDLADFMVVASGGDGGTEVWDFLMALARVEKAIVSGVDGIAVGIGTTLNLHCDLT
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEE
FATPRTVFRTPFVDLGLVPEAGSSLLAPRILGQQGAFALLGLGEGFSAERAKAAGLIYDV
ECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
VEEAALEASVLAAASQIAAKPPQALKIARDLMRGSRDDLVARIGEESEHFRERLKSDEAR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCHHH
AALTAFMTRKKG
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA