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The map label for this gene is purD

Identifier: 13476192

GI number: 13476192

Start: 6154201

End: 6155478

Strand: Direct

Name: purD

Synonym: mlr7447

Alternate gene names: 13476192

Gene position: 6154201-6155478 (Clockwise)

Preceding gene: 13476191

Following gene: 13476195

Centisome position: 87.47

GC content: 66.82

Gene sequence:

>1278_bases
ATGAACGTTCTTCTTCTGGGCTCCGGCGGCCGCGAACATGCGCTTGCCTGGAAGATTGCCGCCTCGCCGCTGCTGACCAA
ACTGTACGCGGCCCCCGGCAATCCGGGCATAGGTGCTGAGGCTGAGCTGGTGAAGCTTGATATCACCGACCACGCCGCCG
TGACCGCCTTCTGCCAGGAGAAGAAAATCGACCTCGTCGTGGTTGGTCCGGAAGGGCCGCTTGTCGCCGGTATCGCGGAC
GATTTGCGCGCCGAAAACATCCGCGTCTTCGGGCCTTCGAAGGCCGCCGCGCGGCTGGAGGGTTCGAAGGGTTTCACCAA
GGACCTCTGCGCCCGATACAACATTCCGACCGCCGCCTATGGCCGTTTCAACGATCTGGCCTCAGCCAAGGCCTATGTCG
ACCAGACCGGCGCGCCGATCGTCATCAAGGCCGATGGGCTCGCCGCCGGCAAGGGCGTCACTGTCGCCATGACGTCGGAT
GAAGCACGGGCGGCGCTCGATGCCTGCTTCGAAGGATCCTTCGGCGCTGCCGGAGCGGAGGTGGTGGTCGAGGAATTCAT
GACCGGCGAGGAGGCGAGCTTCTTCTGCCTCTGCGACGGTACGACCGCGCTGCCCTTCGGCACGGCGCAGGACCACAAGC
GTGTCGGCGATGGCGATGTCGGCCCCAACACCGGCGGCATGGGCGCCTATTCGCCGGCGCCGGTGATGACGCCGGAAATG
ATCGAGCGTACCATGCGAGAGATCATCGAGCCGACGATGCGCGGCATGGCCGAGCTTGGCGCGCCCTTCGCCGGCATTCT
CTTCGCCGGGCTGATGATCACGGACAAGGGGCCTAAGCTGATCGAATACAACACCCGCTTCGGCGACCCGGAATGCCAGG
TGCTGATGATGCGGTTGAAGGACGACCTGCTGGTGCTGCTCAACGCCGCCGTCGACGGTCAGCTGGCGCACACGTCCATA
CGCTGGCGCGACGAGGCGGCGCTCACCGTGGTGATGGCGGCGCGGGGCTATCCCGGCACACCGGAAAAAGGCTCGGTTAT
CCGCGGCGTCGAGCAGGCGGCGGGCGAAGGCGTCCAGATCTTCCACGCCGGCACCGCCATCAATGGCGGCGCGCTGGTCG
CCAATGGCGGCCGCGTCCTCAATGTCACGGCGAGCGGCGCCACGGTCGGCGAGGCCCAGAAGCGGGCCTATGCCGCGCTC
GACCGGATCGATTGGCCGGACGGCTTCTGCCGCCGCGACATTGGCTGGCAGGCCGTGGCGCGCGAGCGGGCGAGCTGA

Upstream 100 bases:

>100_bases
AGAACCGTAGCGCAGCGGACATCTAGTCCGTGAGCAACGGAAGCGCAGAAAACGCCATCAGATGGCCGCCAGAGCGGATT
TTCGGCATCAAGAGGTGGCC

Downstream 100 bases:

>100_bases
GATTTCGGAGCGGGGGCTTACGGCCGGCCGCGATCGATTTCGGTCGAACCGGTGCTGACGGGGTCGGCCGCGGCGAAGGC
GGCGTCCAGCTGCTCTGGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNVLLLGSGGREHALAWKIAASPLLTKLYAAPGNPGIGAEAELVKLDITDHAAVTAFCQEKKIDLVVVGPEGPLVAGIAD
DLRAENIRVFGPSKAAARLEGSKGFTKDLCARYNIPTAAYGRFNDLASAKAYVDQTGAPIVIKADGLAAGKGVTVAMTSD
EARAALDACFEGSFGAAGAEVVVEEFMTGEEASFFCLCDGTTALPFGTAQDHKRVGDGDVGPNTGGMGAYSPAPVMTPEM
IERTMREIIEPTMRGMAELGAPFAGILFAGLMITDKGPKLIEYNTRFGDPECQVLMMRLKDDLLVLLNAAVDGQLAHTSI
RWRDEAALTVVMAARGYPGTPEKGSVIRGVEQAAGEGVQIFHAGTAINGGALVANGGRVLNVTASGATVGEAQKRAYAAL
DRIDWPDGFCRRDIGWQAVARERAS

Sequences:

>Translated_425_residues
MNVLLLGSGGREHALAWKIAASPLLTKLYAAPGNPGIGAEAELVKLDITDHAAVTAFCQEKKIDLVVVGPEGPLVAGIAD
DLRAENIRVFGPSKAAARLEGSKGFTKDLCARYNIPTAAYGRFNDLASAKAYVDQTGAPIVIKADGLAAGKGVTVAMTSD
EARAALDACFEGSFGAAGAEVVVEEFMTGEEASFFCLCDGTTALPFGTAQDHKRVGDGDVGPNTGGMGAYSPAPVMTPEM
IERTMREIIEPTMRGMAELGAPFAGILFAGLMITDKGPKLIEYNTRFGDPECQVLMMRLKDDLLVLLNAAVDGQLAHTSI
RWRDEAALTVVMAARGYPGTPEKGSVIRGVEQAAGEGVQIFHAGTAINGGALVANGGRVLNVTASGATVGEAQKRAYAAL
DRIDWPDGFCRRDIGWQAVARERAS
>Mature_425_residues
MNVLLLGSGGREHALAWKIAASPLLTKLYAAPGNPGIGAEAELVKLDITDHAAVTAFCQEKKIDLVVVGPEGPLVAGIAD
DLRAENIRVFGPSKAAARLEGSKGFTKDLCARYNIPTAAYGRFNDLASAKAYVDQTGAPIVIKADGLAAGKGVTVAMTSD
EARAALDACFEGSFGAAGAEVVVEEFMTGEEASFFCLCDGTTALPFGTAQDHKRVGDGDVGPNTGGMGAYSPAPVMTPEM
IERTMREIIEPTMRGMAELGAPFAGILFAGLMITDKGPKLIEYNTRFGDPECQVLMMRLKDDLLVLLNAAVDGQLAHTSI
RWRDEAALTVVMAARGYPGTPEKGSVIRGVEQAAGEGVQIFHAGTAINGGALVANGGRVLNVTASGATVGEAQKRAYAAL
DRIDWPDGFCRRDIGWQAVARERAS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=45.6264775413712, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=45.6264775413712, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=45.6264775413712, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=45.6264775413712, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=55.3738317757009, Blast_Score=460, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17567511, Length=420, Percent_Identity=40, Blast_Score=298, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=40.6741573033708, Blast_Score=311, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24582400, Length=407, Percent_Identity=42.014742014742, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI62471619, Length=407, Percent_Identity=42.014742014742, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_RHILO (Q986A5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107762.1
- ProteinModelPortal:   Q986A5
- SMR:   Q986A5
- GeneID:   1230417
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7447
- NMPDR:   fig|266835.1.peg.5863
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 44487; Mature: 44487

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLLLGSGGREHALAWKIAASPLLTKLYAAPGNPGIGAEAELVKLDITDHAAVTAFCQE
CEEEEECCCCCCCEEEEEEHHCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHC
KKIDLVVVGPEGPLVAGIADDLRAENIRVFGPSKAAARLEGSKGFTKDLCARYNIPTAAY
CCCCEEEECCCCCEEECCHHHHCCCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCHHC
GRFNDLASAKAYVDQTGAPIVIKADGLAAGKGVTVAMTSDEARAALDACFEGSFGAAGAE
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHH
VVVEEFMTGEEASFFCLCDGTTALPFGTAQDHKRVGDGDVGPNTGGMGAYSPAPVMTPEM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IERTMREIIEPTMRGMAELGAPFAGILFAGLMITDKGPKLIEYNTRFGDPECQVLMMRLK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEEECCCCCCHHHHHHHHHHH
DDLLVLLNAAVDGQLAHTSIRWRDEAALTVVMAARGYPGTPEKGSVIRGVEQAAGEGVQI
CCEEEEEECCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEE
FHAGTAINGGALVANGGRVLNVTASGATVGEAQKRAYAALDRIDWPDGFCRRDIGWQAVA
EEECCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHH
RERAS
HHCCC
>Mature Secondary Structure
MNVLLLGSGGREHALAWKIAASPLLTKLYAAPGNPGIGAEAELVKLDITDHAAVTAFCQE
CEEEEECCCCCCCEEEEEEHHCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHC
KKIDLVVVGPEGPLVAGIADDLRAENIRVFGPSKAAARLEGSKGFTKDLCARYNIPTAAY
CCCCEEEECCCCCEEECCHHHHCCCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCHHC
GRFNDLASAKAYVDQTGAPIVIKADGLAAGKGVTVAMTSDEARAALDACFEGSFGAAGAE
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHH
VVVEEFMTGEEASFFCLCDGTTALPFGTAQDHKRVGDGDVGPNTGGMGAYSPAPVMTPEM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IERTMREIIEPTMRGMAELGAPFAGILFAGLMITDKGPKLIEYNTRFGDPECQVLMMRLK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEEECCCCCCHHHHHHHHHHH
DDLLVLLNAAVDGQLAHTSIRWRDEAALTVVMAARGYPGTPEKGSVIRGVEQAAGEGVQI
CCEEEEEECCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEE
FHAGTAINGGALVANGGRVLNVTASGATVGEAQKRAYAALDRIDWPDGFCRRDIGWQAVA
EEECCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHH
RERAS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968