Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 13476182

GI number: 13476182

Start: 6143972

End: 6146128

Strand: Direct

Name: glyS

Synonym: mlr7435

Alternate gene names: 13476182

Gene position: 6143972-6146128 (Clockwise)

Preceding gene: 13476181

Following gene: 13476186

Centisome position: 87.32

GC content: 63.98

Gene sequence:

>2157_bases
ATGCCTGACCTGCTTCTAGAACTTCGCTCCGAGGAAATCCCCGCGCGCATGCAGCGCAAGGCGGCGGGTGATCTCAAGAA
GATGCTGACCGACGGTCTGGTCGAGGCGGGGCTGACCTACGAAGCGGCGCGCGAATACTGGACGCCGCGGCGTCTGACGC
TCGACATCAGGGGCCTGACGGCGCGCTCGAAGGACATTCGTGAAGAGATCAAGGGGCCGTCGACGACGGCGCCTGAGCAG
GCGGTCCAAGGGTTCCTGCGCAAGGCAGGGCTGTCCTCGGTCGCCGACGCGCATGTCCATTCCGACCCGAAGAAGGGCGA
TTTCTATGTCGCCCACATTTCGAAGCCGGGCAGGGCGGCCGAAGAGATCATCGCCCAACTGGTGCCGGGCATCATCCGCA
ACTTTCCTTGGCCGAAGTCGATGCGCTGGGGACCCGCCTCGGCCAAGCCGGGGTCGCTGCGCTGGGTGCGGCCGCTGCAA
TCGGTCCTGTGCACCTTCGGCCCGGAAACCGAAGAACCCGTCGTGGTCGATTTCGAGATCGACGGCATCCGCTCCGGCAA
CATCACCTACGGCCACCGCTTCCTGGCACCGGGCGAAATCACCGTGCGCCGCTTCGACGATTATGTAAGCAAGCTGGAAG
CGGCGAAGGTCGTGCTCGACGCCGACCGGCGCAAGGAGATCATCCTTGCCGACGCCCGCAACCTTGCCTTCGCCAATGGG
CTCGACCTGGTCGAGGACGAAGGCTTGCTTGAAGAGGTGTCCGGGCTGGTCGAATGGCCCGTGGTGCTGATGGGGGAGTT
CGAAGAGGCTTTTCTCGCCATTCCGGCCGAGGTGATCCGGTTGACCATCCGCGCCAACCAGAAGTGCTTTGTCACGCGCC
CGCAAGGCGAGGGCGAGGCTCTGTCCAACCGCTTCATCCTCACCTCCAATATCGAGGCCAGGGACGGCGGCAAGGAGATC
GCGCACGGCAACGGCAAGGTAGTGCGCGCCCGCCTGTCCGACGCGCTCTATTTCTGGACGACCGACCAGGGCGATCTGCC
TGACCTCGGCCAGCTCGAAGCCTCGGCGGAAAAGTTTGGGCTCGATCTGAACAAGCCGCTCGACCAGCGCATGGCACGGC
TCGATCATCTCAATGTGACCTTCCATGCCAAGCTCGGGACCCAGGGCGAGCGGGTGGAGCGGATCAGGCGGCTCGCCGAA
GAACTGGCGCCGACCGTCGGTGCTGACCCGGTGCTCGCCGCTCGCGCAGCCGTTCTGGCCAAGGCCGACCTGCAGACCGA
GGTGGTCGGCGAGTTCCCGGAATTGCAGGGCGCCATGGGTCGCAAATATGCGCTGCTGCAGGGCGAACACCCGTCCGTGG
CCGCGGCCATCGAGGAACATTACAAGCCGCAAGGCCCGTCCGACTACGTTCCGAGCGACCCGGTGTCGGTCGCTGTCGCG
CTTGCCGACAAGCTCGATACGCTGGTCGGCTTCTGGGCCATCGATGAAAAGCCGACAGGGTCGAAGGACCCATATGCGTT
GCGTCGCGCAGCGCTGGGCGTGGTGAGAATTCTGGTTGAGGACCGAATTCAATTGCGGCTGTCTTCCATCTTCGCGAGCG
CCGGCGCGTGCTACGCGGGCAGTGGTGCGGACCAGACACGAGACCTCCTGGCCTTCTTCCACGACCGCCTGAAGGTCTAC
CTCCGCGACCAGGGCGCCCGCCACGATTTGATAGATGCCGTCATCACCCCGCAGTCCGACGACCTCTTGCAGATCGTCCG
CCGCGTCGAAGCGCTCGGCTCCTTCCTCGACACCGAGGATGGCAAGAATCTGCTCGCCGGCACCAAGCGCGCGGCCAACA
TCCTGGCCGCCGAGGAGAAGAAGAAAACGGCGGTCGCCAAGACCGTTGAGCCGGCTCTGTTCAAGGAGAATGCCGAGAAG
TCGCTGTTTGCGGCGGTGAATCAGGCCGAGAAGCAAGCCGGCGAAGCGATTCAAAACGAAGACTTTTCCGCCGCCATGCT
GGCGCTTAGCGCGTTGCGCGAACCCGTTGATTCATTCTTTGAAGGTGTTCTAGTGAATGATGAAGACCTGGAGGTGCGAG
CCAATCGTCTGGCGCTCCTCACACGCATCCGCGCGGCCACGGGTCAGGTCGCGGATTTCTCGAAAATCGCCGGCTGA

Upstream 100 bases:

>100_bases
CGCGTCCCGACAGGGCAGCCAAAATTTCCGGAAAGGGTGACAGCGGCCAGCCGAGTTGCTATGCCCCGCCCGGACCACTC
TCCCGCATGAGCTGACCCCA

Downstream 100 bases:

>100_bases
GCGCCCAGGAAAAATATCTCCCAGCCGGCCGTTCGAGCCGGCTTACAGTCATATGACGATGATCTCTCCGTGGCAAAAGG
GACATGTTCCCCAACCCAAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MPDLLLELRSEEIPARMQRKAAGDLKKMLTDGLVEAGLTYEAAREYWTPRRLTLDIRGLTARSKDIREEIKGPSTTAPEQ
AVQGFLRKAGLSSVADAHVHSDPKKGDFYVAHISKPGRAAEEIIAQLVPGIIRNFPWPKSMRWGPASAKPGSLRWVRPLQ
SVLCTFGPETEEPVVVDFEIDGIRSGNITYGHRFLAPGEITVRRFDDYVSKLEAAKVVLDADRRKEIILADARNLAFANG
LDLVEDEGLLEEVSGLVEWPVVLMGEFEEAFLAIPAEVIRLTIRANQKCFVTRPQGEGEALSNRFILTSNIEARDGGKEI
AHGNGKVVRARLSDALYFWTTDQGDLPDLGQLEASAEKFGLDLNKPLDQRMARLDHLNVTFHAKLGTQGERVERIRRLAE
ELAPTVGADPVLAARAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEHPSVAAAIEEHYKPQGPSDYVPSDPVSVAVA
LADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVVRILVEDRIQLRLSSIFASAGACYAGSGADQTRDLLAFFHDRLKVY
LRDQGARHDLIDAVITPQSDDLLQIVRRVEALGSFLDTEDGKNLLAGTKRAANILAAEEKKKTAVAKTVEPALFKENAEK
SLFAAVNQAEKQAGEAIQNEDFSAAMLALSALREPVDSFFEGVLVNDEDLEVRANRLALLTRIRAATGQVADFSKIAG

Sequences:

>Translated_718_residues
MPDLLLELRSEEIPARMQRKAAGDLKKMLTDGLVEAGLTYEAAREYWTPRRLTLDIRGLTARSKDIREEIKGPSTTAPEQ
AVQGFLRKAGLSSVADAHVHSDPKKGDFYVAHISKPGRAAEEIIAQLVPGIIRNFPWPKSMRWGPASAKPGSLRWVRPLQ
SVLCTFGPETEEPVVVDFEIDGIRSGNITYGHRFLAPGEITVRRFDDYVSKLEAAKVVLDADRRKEIILADARNLAFANG
LDLVEDEGLLEEVSGLVEWPVVLMGEFEEAFLAIPAEVIRLTIRANQKCFVTRPQGEGEALSNRFILTSNIEARDGGKEI
AHGNGKVVRARLSDALYFWTTDQGDLPDLGQLEASAEKFGLDLNKPLDQRMARLDHLNVTFHAKLGTQGERVERIRRLAE
ELAPTVGADPVLAARAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEHPSVAAAIEEHYKPQGPSDYVPSDPVSVAVA
LADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVVRILVEDRIQLRLSSIFASAGACYAGSGADQTRDLLAFFHDRLKVY
LRDQGARHDLIDAVITPQSDDLLQIVRRVEALGSFLDTEDGKNLLAGTKRAANILAAEEKKKTAVAKTVEPALFKENAEK
SLFAAVNQAEKQAGEAIQNEDFSAAMLALSALREPVDSFFEGVLVNDEDLEVRANRLALLTRIRAATGQVADFSKIAG
>Mature_717_residues
PDLLLELRSEEIPARMQRKAAGDLKKMLTDGLVEAGLTYEAAREYWTPRRLTLDIRGLTARSKDIREEIKGPSTTAPEQA
VQGFLRKAGLSSVADAHVHSDPKKGDFYVAHISKPGRAAEEIIAQLVPGIIRNFPWPKSMRWGPASAKPGSLRWVRPLQS
VLCTFGPETEEPVVVDFEIDGIRSGNITYGHRFLAPGEITVRRFDDYVSKLEAAKVVLDADRRKEIILADARNLAFANGL
DLVEDEGLLEEVSGLVEWPVVLMGEFEEAFLAIPAEVIRLTIRANQKCFVTRPQGEGEALSNRFILTSNIEARDGGKEIA
HGNGKVVRARLSDALYFWTTDQGDLPDLGQLEASAEKFGLDLNKPLDQRMARLDHLNVTFHAKLGTQGERVERIRRLAEE
LAPTVGADPVLAARAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEHPSVAAAIEEHYKPQGPSDYVPSDPVSVAVAL
ADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVVRILVEDRIQLRLSSIFASAGACYAGSGADQTRDLLAFFHDRLKVYL
RDQGARHDLIDAVITPQSDDLLQIVRRVEALGSFLDTEDGKNLLAGTKRAANILAAEEKKKTAVAKTVEPALFKENAEKS
LFAAVNQAEKQAGEAIQNEDFSAAMLALSALREPVDSFFEGVLVNDEDLEVRANRLALLTRIRAATGQVADFSKIAG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=721, Percent_Identity=35.0901525658807, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_RHILO (Q986B5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107752.1
- ProteinModelPortal:   Q986B5
- GeneID:   1230407
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7435
- NMPDR:   fig|266835.1.peg.5853
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- ProtClustDB:   PRK01233
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78611; Mature: 78480

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELRSEEIPARMQRKAAGDLKKMLTDGLVEAGLTYEAAREYWTPRRLTLDIRGLT
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCC
ARSKDIREEIKGPSTTAPEQAVQGFLRKAGLSSVADAHVHSDPKKGDFYVAHISKPGRAA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHH
EEIIAQLVPGIIRNFPWPKSMRWGPASAKPGSLRWVRPLQSVLCTFGPETEEPVVVDFEI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEE
DGIRSGNITYGHRFLAPGEITVRRFDDYVSKLEAAKVVLDADRRKEIILADARNLAFANG
CCCCCCCEEECCEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCC
LDLVEDEGLLEEVSGLVEWPVVLMGEFEEAFLAIPAEVIRLTIRANQKCFVTRPQGEGEA
CCCCCCCCHHHHHHHHHHCHHHHHCCHHHHHHHCCHHHHEEEEECCCEEEEECCCCCCCH
LSNRFILTSNIEARDGGKEIAHGNGKVVRARLSDALYFWTTDQGDLPDLGQLEASAEKFG
HCCCEEEECCCCCCCCCCHHCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHC
LDLNKPLDQRMARLDHLNVTFHAKLGTQGERVERIRRLAEELAPTVGADPVLAARAAVLA
CCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KADLQTEVVGEFPELQGAMGRKYALLQGEHPSVAAAIEEHYKPQGPSDYVPSDPVSVAVA
HHCCHHHHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVVRILVEDRIQLRLSSIFASAGACYAG
HHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGADQTRDLLAFFHDRLKVYLRDQGARHDLIDAVITPQSDDLLQIVRRVEALGSFLDTED
CCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GKNLLAGTKRAANILAAEEKKKTAVAKTVEPALFKENAEKSLFAAVNQAEKQAGEAIQNE
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
DFSAAMLALSALREPVDSFFEGVLVNDEDLEVRANRLALLTRIRAATGQVADFSKIAG
CHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
PDLLLELRSEEIPARMQRKAAGDLKKMLTDGLVEAGLTYEAAREYWTPRRLTLDIRGLT
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCC
ARSKDIREEIKGPSTTAPEQAVQGFLRKAGLSSVADAHVHSDPKKGDFYVAHISKPGRAA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHH
EEIIAQLVPGIIRNFPWPKSMRWGPASAKPGSLRWVRPLQSVLCTFGPETEEPVVVDFEI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEE
DGIRSGNITYGHRFLAPGEITVRRFDDYVSKLEAAKVVLDADRRKEIILADARNLAFANG
CCCCCCCEEECCEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCC
LDLVEDEGLLEEVSGLVEWPVVLMGEFEEAFLAIPAEVIRLTIRANQKCFVTRPQGEGEA
CCCCCCCCHHHHHHHHHHCHHHHHCCHHHHHHHCCHHHHEEEEECCCEEEEECCCCCCCH
LSNRFILTSNIEARDGGKEIAHGNGKVVRARLSDALYFWTTDQGDLPDLGQLEASAEKFG
HCCCEEEECCCCCCCCCCHHCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHC
LDLNKPLDQRMARLDHLNVTFHAKLGTQGERVERIRRLAEELAPTVGADPVLAARAAVLA
CCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KADLQTEVVGEFPELQGAMGRKYALLQGEHPSVAAAIEEHYKPQGPSDYVPSDPVSVAVA
HHCCHHHHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVVRILVEDRIQLRLSSIFASAGACYAG
HHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGADQTRDLLAFFHDRLKVYLRDQGARHDLIDAVITPQSDDLLQIVRRVEALGSFLDTED
CCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GKNLLAGTKRAANILAAEEKKKTAVAKTVEPALFKENAEKSLFAAVNQAEKQAGEAIQNE
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
DFSAAMLALSALREPVDSFFEGVLVNDEDLEVRANRLALLTRIRAATGQVADFSKIAG
CHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968