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The map label for this gene is ispB [H]

Identifier: 13476175

GI number: 13476175

Start: 6136946

End: 6137962

Strand: Direct

Name: ispB [H]

Synonym: mlr7426

Alternate gene names: 13476175

Gene position: 6136946-6137962 (Clockwise)

Preceding gene: 13476169

Following gene: 13476176

Centisome position: 87.22

GC content: 63.91

Gene sequence:

>1017_bases
GTGGGTGTCGTTCTCAACATCGAAAACGGGAAGCGGGAGCCCGCCTCCATCAAGGATCTGATCGATCTGACGGCGGCCGA
TATGGGACGCGTCAACGAATTGATCCTGTCCAAGGCCGGCTCCGACGTCGAGATGATTCCGGAGGTCGCCAACCATCTGA
TCTCGTCCGGCGGCAAGCGGCTCAGGCCGATGCTGACGCTCGCCGCCGCGCAGATGTTCGGCTATGCCGGCGAAGGCCAC
GTCAAGCTCGCCACCTCGGTGGAATTCATGCACACCGCGACGCTGCTGCACGACGACGTCGTCGACGAGAGCGGCATGCG
CCGCGGCAAGAAGACCGCCCGCATGATCTGGGGCAACCAGGCGAGCGTGCTGGTTGGCGATTTCCTGCTTGGCCAGGCCT
TCCGTATGATGGTCGATGTCGGCTCGCTCGAAGCGCTCGACATCCTGTCGAGCGCCGCCTCGATCATCGCCGAGGGCGAG
GTCATGCAGCTCGCCGCCGCCAAGAACCTCGAAACCACCGAAGACGAGCACTTCGCCGTCATCAAGGCCAAGACCGCAGC
ACTTTTTTCGGCGGCCGCCGAAGTTGGACCGGTCATCGCCCAGGCGACCCGCAATGATCGCGCAGCACTGCGCTCCTACG
GCATGAATCTCGGGCTTGCCTTCCAGCTCATCGACGATGCGCTGGACTATGGCGGCACCAGCAAGGATCTGGGCAAGAAT
GTCGGCGACGATTTCCGCGAGGGCAAGGTGACCTTGCCGGTCATCCTCGCCTATCGGCGCGGCACCAAGGCCGAGCGCAC
CTTCTGGAAGCGCGCCATCGAGGACAATGTCGTTGACGATGCGGGCCTGGAAAAGGCGATCGGCCTGATGACCCGGCACG
GCGCCATCGCCGACACGATCGGCCGTGCCCGGCATTTCGGCGAGATCGCCCGCGACGCGCTCGCGCCGCTCGAAGAAACG
CGTCAGAAATCGGCGCTGATCGACGTCATCGATTTCTGCATCAGCCGGGTGAACTGA

Upstream 100 bases:

>100_bases
GCCAATTCGCCACTTGCCGTGGCCGTGTCCGCCGCCCTAGAGTCTGGATCGGGATTAAGGGTGCCGTCGCACGCGTTATA
ACAAGACGGGAGTGATTGTC

Downstream 100 bases:

>100_bases
GAAGGCCGACTTTTCGCCGAATTTCCCCTCGCTTTGTACTGGGGAGAACCTTGGCGAACACAGCCTCGCCGTCTCGACAA
GCGGCCTTGCGGCAAATTAT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGVVLNIENGKREPASIKDLIDLTAADMGRVNELILSKAGSDVEMIPEVANHLISSGGKRLRPMLTLAAAQMFGYAGEGH
VKLATSVEFMHTATLLHDDVVDESGMRRGKKTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLEALDILSSAASIIAEGE
VMQLAAAKNLETTEDEHFAVIKAKTAALFSAAAEVGPVIAQATRNDRAALRSYGMNLGLAFQLIDDALDYGGTSKDLGKN
VGDDFREGKVTLPVILAYRRGTKAERTFWKRAIEDNVVDDAGLEKAIGLMTRHGAIADTIGRARHFGEIARDALAPLEET
RQKSALIDVIDFCISRVN

Sequences:

>Translated_338_residues
MGVVLNIENGKREPASIKDLIDLTAADMGRVNELILSKAGSDVEMIPEVANHLISSGGKRLRPMLTLAAAQMFGYAGEGH
VKLATSVEFMHTATLLHDDVVDESGMRRGKKTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLEALDILSSAASIIAEGE
VMQLAAAKNLETTEDEHFAVIKAKTAALFSAAAEVGPVIAQATRNDRAALRSYGMNLGLAFQLIDDALDYGGTSKDLGKN
VGDDFREGKVTLPVILAYRRGTKAERTFWKRAIEDNVVDDAGLEKAIGLMTRHGAIADTIGRARHFGEIARDALAPLEET
RQKSALIDVIDFCISRVN
>Mature_337_residues
GVVLNIENGKREPASIKDLIDLTAADMGRVNELILSKAGSDVEMIPEVANHLISSGGKRLRPMLTLAAAQMFGYAGEGHV
KLATSVEFMHTATLLHDDVVDESGMRRGKKTARMIWGNQASVLVGDFLLGQAFRMMVDVGSLEALDILSSAASIIAEGEV
MQLAAAKNLETTEDEHFAVIKAKTAALFSAAAEVGPVIAQATRNDRAALRSYGMNLGLAFQLIDDALDYGGTSKDLGKNV
GDDFREGKVTLPVILAYRRGTKAERTFWKRAIEDNVVDDAGLEKAIGLMTRHGAIADTIGRARHFGEIARDALAPLEETR
QKSALIDVIDFCISRVN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=28.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=45.1713395638629, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1786623, Length=241, Percent_Identity=33.195020746888, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=301, Percent_Identity=28.9036544850498, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319475, Length=293, Percent_Identity=31.0580204778157, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325188, Length=243, Percent_Identity=21.3991769547325, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651612, Length=315, Percent_Identity=32.0634920634921, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281365769, Length=226, Percent_Identity=24.7787610619469, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660002, Length=226, Percent_Identity=24.7787610619469, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36395; Mature: 36264

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVLNIENGKREPASIKDLIDLTAADMGRVNELILSKAGSDVEMIPEVANHLISSGGKR
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLAAAQMFGYAGEGHVKLATSVEFMHTATLLHDDVVDESGMRRGKKTARMIWGNQ
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
ASVLVGDFLLGQAFRMMVDVGSLEALDILSSAASIIAEGEVMQLAAAKNLETTEDEHFAV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEE
IKAKTAALFSAAAEVGPVIAQATRNDRAALRSYGMNLGLAFQLIDDALDYGGTSKDLGKN
EEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHCC
VGDDFREGKVTLPVILAYRRGTKAERTFWKRAIEDNVVDDAGLEKAIGLMTRHGAIADTI
CCCHHHCCCEEEHHHEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHH
GRARHFGEIARDALAPLEETRQKSALIDVIDFCISRVN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVVLNIENGKREPASIKDLIDLTAADMGRVNELILSKAGSDVEMIPEVANHLISSGGKR
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLAAAQMFGYAGEGHVKLATSVEFMHTATLLHDDVVDESGMRRGKKTARMIWGNQ
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
ASVLVGDFLLGQAFRMMVDVGSLEALDILSSAASIIAEGEVMQLAAAKNLETTEDEHFAV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEE
IKAKTAALFSAAAEVGPVIAQATRNDRAALRSYGMNLGLAFQLIDDALDYGGTSKDLGKN
EEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHCC
VGDDFREGKVTLPVILAYRRGTKAERTFWKRAIEDNVVDDAGLEKAIGLMTRHGAIADTI
CCCHHHCCCEEEHHHEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHH
GRARHFGEIARDALAPLEETRQKSALIDVIDFCISRVN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]