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The map label for this gene is alkK [H]

Identifier: 13476075

GI number: 13476075

Start: 6028911

End: 6030551

Strand: Direct

Name: alkK [H]

Synonym: mlr7293

Alternate gene names: 13476075

Gene position: 6028911-6030551 (Clockwise)

Preceding gene: 13476072

Following gene: 13476076

Centisome position: 85.69

GC content: 63.92

Gene sequence:

>1641_bases
ATGGCCAATTCTTACGAACAGGATCTCGACAGGAACGCGGCCAACCACCAGCCGCTGACGCCGCTCACCTATCTGGAGCG
CGCGGCAAAGACCTATCCCGACCACATCGCCATCATCCACGGCCGCCAGCGCATCAGCTACCGCGATTTCTGGCGCCGCT
CGCTGAAGGTCGCATCGGCACTGCAAAAGCGCGGCATCGGCAAGGGCGACACGGTCACCGTGATGCTGTCCAACACGCCG
CCGATGCTGGAGGCGCATTTCGGTGTGCCGATGACCAAGGCGGTGCTGCATTCGCTCAACACCCGCCTCGACGCCGCGGT
CATCGCCTTCCAGCTCGACCATGCCGAGACCAAGGTGCTCATCGTCGACCGCGAATTCTCCGGCGTCGTCCGGCAGGCGC
TCGATCTGGCCAAGGTCACGCCGCTGGTCATCGACTATGACGATCCCGACTACGCCGCCGACGCCCCCTATCCGAAGGGG
GAACGGATTGGCGCGCTCGACTATGAGGACTTTGTCGCCGGTGGCGACGAGGATTTCGCCTGGTCGATGCCCGACGATGA
ATGGGACGCCATCTCGCTCAACTACACCTCGGGCACGACGGGCAATCCGAAGGGCGTGGTCTACCACCATCGCGGTGCCG
CCCTGATGGCCTACACCAACACCATCCATGCCGGCATGGCCAAGCACGCCGTCTATCTCTGGACGCTTCCGATGTTCCAC
TGCAATGGCTGGTGCTTTCCGTGGACGCTGGCCGTCCAGGCCGGCACCCATGTCTGCCTGCGCTGGGTGCGGCCGAAGCC
GATCTATGACGCCATCGCCGATCATGGCGTCACACATCTCTGCGGCGCGCCGGTCGTCATGTCGGTGCTGATCAACGCCC
GGGATGAGGACAAGCGCACGTTCGCGCAGACGGTGACCTTCAACACCGCGGCCGCGCCGCCGCCGGAAGCCGTGCTGTCG
GGGATGGCCGATGCCGGCTTTGCCGTCACCCATCTCTATGGCCTGACCGAGACCTATGGCCCGGCCGTGGTCAACGAATG
GCATGGCGAATGGGACGATCTCGCAAAGGGCGAGCGCAGCGCCAAGAAGGCAAGGCAGGGCGTGCGCTATGCCGCGCTCG
AAGGCCTGACCGTCATGGACCCCGAGACGATGCAGGCGACGCCGGCCGACGGCGAGACCATCGGCGAGGTCATGTTCCGC
GGCAACATCGTCATGAAGGGCTACCTCAAGAACCGCAAGGCCAGCGACGAAGCGTTCGCCGGCGGCTGGTTCCATTCCGG
TGATCTCGGCGTCATGCATCCCGATGGCTACATCCAGCTCAAGGACCGTTCCAAGGACATCATCATTTCGGGCGGCGAGA
ACATTTCCTCGATCGAGGTCGAGGATGCGCTCTACAAGCACCCGTCCGTCGCCTCCTGCGGCGTTGTGGCGCGCCATGAC
GACAAATGGGGGGAGGTGCCGGTTGCCTATGTCGAGCTGAAGCCCGGCAAGACGGCGAGCGAGGCCGAGATCATCGAGCA
CTGCCGTGCGCTGCTTGCCCGCTTCAAAGTGCCGAAAGCGGTGATTTTTGCCGAGATCCCGAAGACCTCGACCGGCAAGA
TCCAGAAATTCCGACTCCGCGAAATGGCCAAACTCGCCTGA

Upstream 100 bases:

>100_bases
TACAAAGCCTGGACAGCATAGCATCTTCGGCCATTCAGGGGGATGGCCTGACGAGAGCCCTAAGGTTAGAAATCTCGAAA
AGGACTTTGGGGAGGATGGG

Downstream 100 bases:

>100_bases
AGCGCCGGCGGCCTCTTTAAAAAATATTTTGAGTTGCCCGCATCCTTGGGCCGCTTGGTTACGTCTGTTGGATGTCACCA
TCTGTCACTATCTTGTACGC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MANSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTP
PMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKG
ERIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFH
CNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLS
GMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFR
GNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHD
DKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAKLA

Sequences:

>Translated_546_residues
MANSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTP
PMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKG
ERIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFH
CNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLS
GMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFR
GNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHD
DKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAKLA
>Mature_545_residues
ANSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPP
MLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKGE
RIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHC
NGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSG
MADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRG
NIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD
KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAKLA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=577, Percent_Identity=27.209705372617, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI187761345, Length=559, Percent_Identity=25.7602862254025, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI187761343, Length=559, Percent_Identity=25.7602862254025, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI157311624, Length=542, Percent_Identity=23.8007380073801, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311622, Length=542, Percent_Identity=23.8007380073801, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI58082049, Length=540, Percent_Identity=23.3333333333333, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=24.0458015267176, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI42544132, Length=525, Percent_Identity=24.3809523809524, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=24.6628131021195, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI38505220, Length=536, Percent_Identity=23.8805970149254, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI13375727, Length=564, Percent_Identity=25.177304964539, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788107, Length=574, Percent_Identity=24.390243902439, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=27.3244781783681, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=24.4575936883629, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786810, Length=546, Percent_Identity=27.1062271062271, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=566, Percent_Identity=27.7385159010601, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71994703, Length=566, Percent_Identity=27.7385159010601, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71994690, Length=566, Percent_Identity=27.7385159010601, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17558820, Length=518, Percent_Identity=28.3783783783784, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=525, Percent_Identity=26.6666666666667, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=26.5799256505576, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=26.3358778625954, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=542, Percent_Identity=23.4317343173432, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=526, Percent_Identity=25.0950570342205, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=533, Percent_Identity=21.9512195121951, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=522, Percent_Identity=24.5210727969349, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=530, Percent_Identity=22.8301886792453, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=213, Percent_Identity=30.9859154929577, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=569, Percent_Identity=22.3198594024605, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=569, Percent_Identity=22.3198594024605, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=538, Percent_Identity=24.7211895910781, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=582, Percent_Identity=24.3986254295533, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=138, Percent_Identity=29.7101449275362, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581924, Length=567, Percent_Identity=26.63139329806, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355181, Length=545, Percent_Identity=24.2201834862385, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18859661, Length=534, Percent_Identity=26.2172284644195, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356947, Length=572, Percent_Identity=20.2797202797203, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648260, Length=514, Percent_Identity=21.2062256809339, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921316, Length=554, Percent_Identity=21.841155234657, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=21.8503937007874, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648255, Length=508, Percent_Identity=21.8503937007874, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653035, Length=514, Percent_Identity=19.6498054474708, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583465, Length=239, Percent_Identity=24.6861924686192, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583463, Length=239, Percent_Identity=24.6861924686192, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364798, Length=239, Percent_Identity=24.6861924686192, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552801, Length=238, Percent_Identity=25.6302521008403, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550496, Length=238, Percent_Identity=25.6302521008403, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130357, Length=151, Percent_Identity=27.8145695364238, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60133; Mature: 60002

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASA
CCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVL
HHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEECCCCEEEE
IVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDFA
EEECHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEECCCCHHHHHCCCCCCCE
WSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFH
ECCCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHCEEEEEEEHHHH
CNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRT
CCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHCCCHHHHCHHHHHHHHHCCCCCHHHH
FAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERS
HHHEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCHHH
AKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKASDEAFA
HHHHHCCCEEHHHCCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCC
GGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHD
CCCEECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCCCCEEEECC
DKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLR
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
EMAKLA
HHHHCC
>Mature Secondary Structure 
ANSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASA
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
LQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVL
HHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEECCCCEEEE
IVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDFA
EEECHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEECCCCHHHHHCCCCCCCE
WSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFH
ECCCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHCEEEEEEEHHHH
CNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRT
CCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHCCCHHHHCHHHHHHHHHCCCCCHHHH
FAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERS
HHHEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCHHH
AKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKASDEAFA
HHHHHCCCEEHHHCCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCC
GGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHD
CCCEECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCCCCEEEECC
DKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLR
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
EMAKLA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]