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The map label for this gene is dhaK [H]

Identifier: 13476064

GI number: 13476064

Start: 6015580

End: 6016593

Strand: Reverse

Name: dhaK [H]

Synonym: mll7280

Alternate gene names: 13476064

Gene position: 6016593-6015580 (Counterclockwise)

Preceding gene: 13476065

Following gene: 13476063

Centisome position: 85.51

GC content: 66.86

Gene sequence:

>1014_bases
ATGCCGGTGCAGACCAAGAAGATCATCAATGACGGCAACCGCGCCGTCGACGAAATGCTCGAAGGGATCCTCGCCGCGCA
TCCTCGCCACCTCAGGAGCGCGGACGGCAGCCCGCGCTCGATCATCGCCCGTGACGGGCCGCGGCCCGGCAAGGTCGGGC
TGGTGATCGGCGGCGGCTCAGGTCATGAGCCGACCTTCCTCGGCTTTGTCGGCAAGGGACTGGCGGATGCGGCGGCCATC
GGCAACGTCTTCGCCTCGCCGCCGCCCGATCCGATCCTCGAATGCGCCAAGGCAGTCAGCGGCGGCGCCGGCGTCTTGTT
CATGTACGGCAACTATGCCGGCGACGTGATGAATTTCGACATGGCGGCCGAGATGGCAGCGATGGACGACATCGAGGTGC
GCACCGTCCTGACCACCGACGACGTCGCCTCGGCGCTGCGCGACCAGCGGCAGAAGCGCCGCGGCGTCGCCGGCAATTTC
TTCATCTTCAAGGCGGCCGGTGCCGCCTGCGACCGGATGCTCTCGCTGGACGAGGTCGAGCGCATCGCCCGGAAGGCCAA
CGACCACACCTTCACCATGGGCGTGGCGCTGTCGCCCTGCTCGCTGCCGCAGACAAGGCGGCCGAATTTCGAGATCGGCG
CGGGCGAGATGGAGATCGGCATGGGCATCCATGGCGAACCCGGCATCGCCCGCGGCAAGCTCGGGAGCGCCGACGCGATC
ACCGACGAGATGCTGGACAAGATCCTCGCCGAGATGGCGCCGACGCGCGGCGACAAGGTCGCGGTGCTGGTCAACTCGCT
CGGCTCGACGCCGCTGATGGAGCTCTACATCATGAACCGGCGGGTCAAGCAGCGGCTCGACGACATCGGCGTCACCATCC
ACGCGACCTGGGTCGGCAATTACTGCACGTCGTTGGAAATGGCCGGCGCCTCGGTGACGCTGTTTCATCTCGACGAGGAA
TTGCAGACCATGCTCGACCATCCCTGCGACTGCGCCATGTTCCGTGCCGGCTGA

Upstream 100 bases:

>100_bases
TGGAAATTCTCGCCGCGCGGGAAAAGATCGCGGCATGAAATCTTCCCGGCCGGGTGGAAATCGCGATAACACCCGGTAGT
ATTTGCGAAAACTCGGGAGG

Downstream 100 bases:

>100_bases
CGAAAGAGCGTTCCCGTGACCATCGATACCGCTGACCTGAAAAGAATGTTCGATACGATCGCGGTGGCGATCGAGGCCGA
CAAGGACCGGCTCTGCCAGC

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MPVQTKKIINDGNRAVDEMLEGILAAHPRHLRSADGSPRSIIARDGPRPGKVGLVIGGGSGHEPTFLGFVGKGLADAAAI
GNVFASPPPDPILECAKAVSGGAGVLFMYGNYAGDVMNFDMAAEMAAMDDIEVRTVLTTDDVASALRDQRQKRRGVAGNF
FIFKAAGAACDRMLSLDEVERIARKANDHTFTMGVALSPCSLPQTRRPNFEIGAGEMEIGMGIHGEPGIARGKLGSADAI
TDEMLDKILAEMAPTRGDKVAVLVNSLGSTPLMELYIMNRRVKQRLDDIGVTIHATWVGNYCTSLEMAGASVTLFHLDEE
LQTMLDHPCDCAMFRAG

Sequences:

>Translated_337_residues
MPVQTKKIINDGNRAVDEMLEGILAAHPRHLRSADGSPRSIIARDGPRPGKVGLVIGGGSGHEPTFLGFVGKGLADAAAI
GNVFASPPPDPILECAKAVSGGAGVLFMYGNYAGDVMNFDMAAEMAAMDDIEVRTVLTTDDVASALRDQRQKRRGVAGNF
FIFKAAGAACDRMLSLDEVERIARKANDHTFTMGVALSPCSLPQTRRPNFEIGAGEMEIGMGIHGEPGIARGKLGSADAI
TDEMLDKILAEMAPTRGDKVAVLVNSLGSTPLMELYIMNRRVKQRLDDIGVTIHATWVGNYCTSLEMAGASVTLFHLDEE
LQTMLDHPCDCAMFRAG
>Mature_336_residues
PVQTKKIINDGNRAVDEMLEGILAAHPRHLRSADGSPRSIIARDGPRPGKVGLVIGGGSGHEPTFLGFVGKGLADAAAIG
NVFASPPPDPILECAKAVSGGAGVLFMYGNYAGDVMNFDMAAEMAAMDDIEVRTVLTTDDVASALRDQRQKRRGVAGNFF
IFKAAGAACDRMLSLDEVERIARKANDHTFTMGVALSPCSLPQTRRPNFEIGAGEMEIGMGIHGEPGIARGKLGSADAIT
DEMLDKILAEMAPTRGDKVAVLVNSLGSTPLMELYIMNRRVKQRLDDIGVTIHATWVGNYCTSLEMAGASVTLFHLDEEL
QTMLDHPCDCAMFRAG

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=337, Percent_Identity=36.2017804154303, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=40.1685393258427, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17565018, Length=325, Percent_Identity=37.8461538461538, Blast_Score=200, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321055, Length=346, Percent_Identity=32.6589595375723, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323570, Length=343, Percent_Identity=32.9446064139942, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35929; Mature: 35798

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVQTKKIINDGNRAVDEMLEGILAAHPRHLRSADGSPRSIIARDGPRPGKVGLVIGGGS
CCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCCCEEEEEEECCC
GHEPTFLGFVGKGLADAAAIGNVFASPPPDPILECAKAVSGGAGVLFMYGNYAGDVMNFD
CCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHCHH
MAAEMAAMDDIEVRTVLTTDDVASALRDQRQKRRGVAGNFFIFKAAGAACDRMLSLDEVE
HHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCHHHHH
RIARKANDHTFTMGVALSPCSLPQTRRPNFEIGAGEMEIGMGIHGEPGIARGKLGSADAI
HHHHHCCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCHHH
TDEMLDKILAEMAPTRGDKVAVLVNSLGSTPLMELYIMNRRVKQRLDDIGVTIHATWVGN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
YCTSLEMAGASVTLFHLDEELQTMLDHPCDCAMFRAG
HHHHHEECCCEEEEEECCHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure 
PVQTKKIINDGNRAVDEMLEGILAAHPRHLRSADGSPRSIIARDGPRPGKVGLVIGGGS
CCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCCCEEEEEEECCC
GHEPTFLGFVGKGLADAAAIGNVFASPPPDPILECAKAVSGGAGVLFMYGNYAGDVMNFD
CCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHCHH
MAAEMAAMDDIEVRTVLTTDDVASALRDQRQKRRGVAGNFFIFKAAGAACDRMLSLDEVE
HHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCHHHHH
RIARKANDHTFTMGVALSPCSLPQTRRPNFEIGAGEMEIGMGIHGEPGIARGKLGSADAI
HHHHHCCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCHHH
TDEMLDKILAEMAPTRGDKVAVLVNSLGSTPLMELYIMNRRVKQRLDDIGVTIHATWVGN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
YCTSLEMAGASVTLFHLDEELQTMLDHPCDCAMFRAG
HHHHHEECCCEEEEEECCHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]