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The map label for this gene is gsiB [H]

Identifier: 13476038

GI number: 13476038

Start: 5986623

End: 5988140

Strand: Reverse

Name: gsiB [H]

Synonym: mll7247

Alternate gene names: 13476038

Gene position: 5988140-5986623 (Counterclockwise)

Preceding gene: 13476039

Following gene: 13476037

Centisome position: 85.11

GC content: 63.44

Gene sequence:

>1518_bases
ATGGCAAGGCATCTCACAAGAACACTCCTGTCGGGCATCACGGTTGCCGCGATGCTCGGCGTGGCTGTGGTTTCGGCTCA
GGCGGCAACGCTGCACATGGCCTGGTCACAGGACGCCACCGGTCTTGACCCGCACAAGCAGACAGCTTTTTCGTCGCTGC
GGCTCCTGGAACTGATCTACGAGCCGCTGGTGCGGGTCGATGCCAGCCTGCAGATCATCCCCGCCATCGCCACCTCGTGG
GCGTTTTCGAAAGACGGCAAGGAGCTGACTTTCAAGCTCGATCCCAAGGCGAAATTCCAGGACGGCACGGCCGTCACTTC
CGCCGACGTCAAGGCCTCGTTCGAACGGATCCTCGACGAGAAAACGGGTGCCGCCGCCCGCGCCAACTTCCTCTCGATCG
CCAGCATCGACACGCCTGATGCGGCAACCGTCGTGTTCCACCTGTCGCAGCCCGACGTGCCGATCCTGACGGCGATGAGC
GACGTCAACGCGGCGATCGTCCCAGCCAGTGAAATCAAGGCCGGCTCGATCGGCACCAAGGCGGTCGGCTCCGGCCCCTT
CAAGCTCGACAAATGGGACCCTAACGCCAAGGAGGTGCTCAGCGCCAACAAGGATTGGGCCAGCGGCGCGACCGGCGTCG
ACGGCATCGAGATCAGCGTGCTGCCCGACGAGGCGGCGATCCTGGCCGCCATGCGCACCAAGCAGATCGATTTCGCGCTA
CTCAACGACCCGCTGGTCGCCACGCTGGTGCCGAAGGAGGCTAGCCTGCAATTGAACCGCGTGCCGGTGCTTGCCTATCA
CGTGCTGCAGCTCAACCCATCGCGCAAGCCGATGACCGAGCTCAAGGTGCGCCAGGCGATCTCTTGCGCCATCGACCGAC
AGGAGGTGCTGGACACCGCTTCGCTCGGCGAAGGCAAGGTCACCGGACCGCTGACCATCCCGGCGCTCGCCACCGATCCG
ACCCAGCTCTTCTGCTACAAGCGCGATGTCGAAAAGGCCAAGAAGCTGATGGCCGAAGCTGGCTTTGCCGACGGCTTTTC
GGCAACCGTGATCGGTGCCACCGGCGAGCCGCCGACCGCGGCGGCGGAGGCGCAGGTCATCCAGTCGCAGCTGGCTGAAA
TCGGCGTCAAGCTCGACATCAAGATGATGGAGCTCAATGTCTATGTCGACGCCTGGCTGAAAGGCGATTTCGACATGGCG
ATCGCGCTCAATGGCGGCCGCGCCGACCCCTACACCATGTACAACCGCTACTGGACCAAGGCGGGCAATCTGCAGAAGGT
GGCCAACTACATCGACGACACGCTGGACAGCCAGATGCAGCAGGGCCGCGCCGAGACCGATCCGGCCAAACGCAAGGCGA
TCTTCGCCGAATTCGAGAAGCACCTCGCCGAGATGTCGCCGTGGATCTGGCTCTACACCTCCTATAGCTACACGGCGCAG
CAGAAAAACATCGCCGGCTTCGTGCCGACGCCGACCGGCACGCTGTTCAGCCTGAGCAAGGTTACCATCCAGCAGTAG

Upstream 100 bases:

>100_bases
AAGCCGCATAGACTGGGCCGATACTCCAAAGCATGTGTGTCTTGGAAGCGGTCCGCAGAAACTGCAAGGCATCAAGTTCA
AATCATAATGGGAGACGAAA

Downstream 100 bases:

>100_bases
CCGCCACAGGTATTGGACAAGAGGACTTCTTCGCGTGAACTATCTGATGCGACGACTGGCGACGTTTCCTCTCGTCCTGC
TTGGCGTGTCCATCCTGGTC

Product: ABC transporter substrate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MARHLTRTLLSGITVAAMLGVAVVSAQAATLHMAWSQDATGLDPHKQTAFSSLRLLELIYEPLVRVDASLQIIPAIATSW
AFSKDGKELTFKLDPKAKFQDGTAVTSADVKASFERILDEKTGAAARANFLSIASIDTPDAATVVFHLSQPDVPILTAMS
DVNAAIVPASEIKAGSIGTKAVGSGPFKLDKWDPNAKEVLSANKDWASGATGVDGIEISVLPDEAAILAAMRTKQIDFAL
LNDPLVATLVPKEASLQLNRVPVLAYHVLQLNPSRKPMTELKVRQAISCAIDRQEVLDTASLGEGKVTGPLTIPALATDP
TQLFCYKRDVEKAKKLMAEAGFADGFSATVIGATGEPPTAAAEAQVIQSQLAEIGVKLDIKMMELNVYVDAWLKGDFDMA
IALNGGRADPYTMYNRYWTKAGNLQKVANYIDDTLDSQMQQGRAETDPAKRKAIFAEFEKHLAEMSPWIWLYTSYSYTAQ
QKNIAGFVPTPTGTLFSLSKVTIQQ

Sequences:

>Translated_505_residues
MARHLTRTLLSGITVAAMLGVAVVSAQAATLHMAWSQDATGLDPHKQTAFSSLRLLELIYEPLVRVDASLQIIPAIATSW
AFSKDGKELTFKLDPKAKFQDGTAVTSADVKASFERILDEKTGAAARANFLSIASIDTPDAATVVFHLSQPDVPILTAMS
DVNAAIVPASEIKAGSIGTKAVGSGPFKLDKWDPNAKEVLSANKDWASGATGVDGIEISVLPDEAAILAAMRTKQIDFAL
LNDPLVATLVPKEASLQLNRVPVLAYHVLQLNPSRKPMTELKVRQAISCAIDRQEVLDTASLGEGKVTGPLTIPALATDP
TQLFCYKRDVEKAKKLMAEAGFADGFSATVIGATGEPPTAAAEAQVIQSQLAEIGVKLDIKMMELNVYVDAWLKGDFDMA
IALNGGRADPYTMYNRYWTKAGNLQKVANYIDDTLDSQMQQGRAETDPAKRKAIFAEFEKHLAEMSPWIWLYTSYSYTAQ
QKNIAGFVPTPTGTLFSLSKVTIQQ
>Mature_504_residues
ARHLTRTLLSGITVAAMLGVAVVSAQAATLHMAWSQDATGLDPHKQTAFSSLRLLELIYEPLVRVDASLQIIPAIATSWA
FSKDGKELTFKLDPKAKFQDGTAVTSADVKASFERILDEKTGAAARANFLSIASIDTPDAATVVFHLSQPDVPILTAMSD
VNAAIVPASEIKAGSIGTKAVGSGPFKLDKWDPNAKEVLSANKDWASGATGVDGIEISVLPDEAAILAAMRTKQIDFALL
NDPLVATLVPKEASLQLNRVPVLAYHVLQLNPSRKPMTELKVRQAISCAIDRQEVLDTASLGEGKVTGPLTIPALATDPT
QLFCYKRDVEKAKKLMAEAGFADGFSATVIGATGEPPTAAAEAQVIQSQLAEIGVKLDIKMMELNVYVDAWLKGDFDMAI
ALNGGRADPYTMYNRYWTKAGNLQKVANYIDDTLDSQMQQGRAETDPAKRKAIFAEFEKHLAEMSPWIWLYTSYSYTAQQ
KNIAGFVPTPTGTLFSLSKVTIQQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=529, Percent_Identity=27.0321361058601, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787762, Length=514, Percent_Identity=28.2101167315175, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=24.031007751938, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789887, Length=503, Percent_Identity=23.8568588469185, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787495, Length=490, Percent_Identity=25.7142857142857, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081878, Length=495, Percent_Identity=25.0505050505051, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789397, Length=470, Percent_Identity=24.468085106383, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54431; Mature: 54300

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHLTRTLLSGITVAAMLGVAVVSAQAATLHMAWSQDATGLDPHKQTAFSSLRLLELIY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EPLVRVDASLQIIPAIATSWAFSKDGKELTFKLDPKAKFQDGTAVTSADVKASFERILDE
HHHHHCCCCEEEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEECHHHHHHHHHHHHH
KTGAAARANFLSIASIDTPDAATVVFHLSQPDVPILTAMSDVNAAIVPASEIKAGSIGTK
CCCCHHHCCEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEHHHCCCCCCCCC
AVGSGPFKLDKWDPNAKEVLSANKDWASGATGVDGIEISVLPDEAAILAAMRTKQIDFAL
CCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEE
LNDPLVATLVPKEASLQLNRVPVLAYHVLQLNPSRKPMTELKVRQAISCAIDRQEVLDTA
ECCCEEEEECCCCCCCEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHH
SLGEGKVTGPLTIPALATDPTQLFCYKRDVEKAKKLMAEAGFADGFSATVIGATGEPPTA
CCCCCCCCCCEEECEECCCCHHEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
AAEAQVIQSQLAEIGVKLDIKMMELNVYVDAWLKGDFDMAIALNGGRADPYTMYNRYWTK
HHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCC
AGNLQKVANYIDDTLDSQMQQGRAETDPAKRKAIFAEFEKHLAEMSPWIWLYTSYSYTAQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEC
QKNIAGFVPTPTGTLFSLSKVTIQQ
CCCCCEECCCCCCCEEEECEEEECC
>Mature Secondary Structure 
ARHLTRTLLSGITVAAMLGVAVVSAQAATLHMAWSQDATGLDPHKQTAFSSLRLLELIY
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EPLVRVDASLQIIPAIATSWAFSKDGKELTFKLDPKAKFQDGTAVTSADVKASFERILDE
HHHHHCCCCEEEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEECHHHHHHHHHHHHH
KTGAAARANFLSIASIDTPDAATVVFHLSQPDVPILTAMSDVNAAIVPASEIKAGSIGTK
CCCCHHHCCEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEHHHCCCCCCCCC
AVGSGPFKLDKWDPNAKEVLSANKDWASGATGVDGIEISVLPDEAAILAAMRTKQIDFAL
CCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEE
LNDPLVATLVPKEASLQLNRVPVLAYHVLQLNPSRKPMTELKVRQAISCAIDRQEVLDTA
ECCCEEEEECCCCCCCEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHH
SLGEGKVTGPLTIPALATDPTQLFCYKRDVEKAKKLMAEAGFADGFSATVIGATGEPPTA
CCCCCCCCCCEEECEECCCCHHEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
AAEAQVIQSQLAEIGVKLDIKMMELNVYVDAWLKGDFDMAIALNGGRADPYTMYNRYWTK
HHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCC
AGNLQKVANYIDDTLDSQMQQGRAETDPAKRKAIFAEFEKHLAEMSPWIWLYTSYSYTAQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEC
QKNIAGFVPTPTGTLFSLSKVTIQQ
CCCCCEECCCCCCCEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA