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The map label for this gene is dctA2

Identifier: 13476029

GI number: 13476029

Start: 5975395

End: 5976723

Strand: Reverse

Name: dctA2

Synonym: mll7237

Alternate gene names: 13476029

Gene position: 5976723-5975395 (Counterclockwise)

Preceding gene: 13476033

Following gene: 13476015

Centisome position: 84.94

GC content: 63.66

Gene sequence:

>1329_bases
ATGCACATCGCAGACCAATCAGGCGCGGCTGCCGCGCAGCGCAAGCCCCTTTATGCCCAACTCTACGTGCAGGTGCTGGT
GGCGATCACCGTCGGCATCCTGCTCGGCCACTACTATCCATCGATCGGCGAGAGCATGAAGCCGCTCGGCGACGCCTTCA
TCAAGCTGGTCAAAATGATCATCGCCCCCGTCATCTTCCTGACGGTGGCGACCGGCATCGCCGGCATGAGCGACCTGCAG
AAGGTCGGCCGCGTCGCCGGCAAGGCGATGCTCTACTTCCTCACCTTCTCGACGCTGGCGCTGATCATCGGCCTCGTCGT
CGCCAATGTCGTACAACCCGGCGCCGGCTTCAACATCGACCCGGCAACACTCGACGCCTCGACCGTCAACACCTATGCTG
CCAAGGCGCATGACCAATCGGTCACCGGATTCCTGATGAACATCATCCCCGGCACGATCGTCGGCGCCTTCGCCGATGGC
GACATCTTGCAGGTGCTGTTCTTCTCGGTGCTGTTCGGCATCGCGCTGGCGCTGGTCGGCGACAAGGGTGCACCAGTGCT
CAACTTCCTGCAGGCGCTGATGGCGCCGATGTTCAAGCTGGTCAGCGTCTTGATGAAGGCCGCCCCCATCGGTGCGTTCG
GCGCCATGGCCTTCACCATCGGCAAATACGGCATCGGCTCGGTCATCAACCTCGCCATGCTGGTCGGCACCTTCTACGCG
ACGTCGCTGCTGTTCGTGTTCGTCGTCCTGGGCGCAGTCTGCCGCTACAACGGCTTTTCCATCCTGTCGCTGCTGCGCTA
CATCAAGGAAGAGCTGCTCTTGGTGCTGGGCACCTCCTCGTCGGAAGCCGCCCTGCCCTCGCTGATGGAAAAGATGGAGA
AGGCCGGCGCCAAGCGCTCGGTGGTCGGCCTGGTCATCCCGACCGGCTATTCCTTCAACCTCGACGGCACCAACATCTAC
ATGACGCTTGCCGCGCTGTTCATCGCGCAGGCGACGAACATCCATCTGTCGATGGGCGACCAGATCCTGTTGCTGCTGGT
GGCGATGCTCTCGTCCAAGGGTGCCGCCGGCATCACTGGAGCCGGCTTCATCACCCTTGCCGCGACGCTTTCGGTGGTGC
CTTCGGTGCCGGTCGCCGGCATGGCGCTGATCCTCGGCGTCGACCGCTTCATGTCGGAATGCCGGGCGCTGACCAACTTC
ATCGGCAATGCCGTCGCCACGCTGGTCGTCGCGCGCTGGGAAGGCGAGCTCGATGAGGCCAAGCTCGCCAGGGCTTTGGC
CGGCACGGCCGACGACAGCCTGCCGGCGGATATCGTTCCCGCCGAGTAA

Upstream 100 bases:

>100_bases
ACAGCGCTTGCAAACTCCTTGCCAGCGGCGGGCGACCGGAACGCCGCGATGGGAGAAGTCACGCAGGGCGGGTTCCGTCC
ATCCGGCCAGGGAGAGTTCG

Downstream 100 bases:

>100_bases
TTCGCACGGTCGTTTAGCGATACGAATGGCCGGAGGCAGCGATGCCTCCGGCCATTTTCATGTGGGTGACGATGTCAGGC
GGCGCGGATCTTGCCGAGCG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MHIADQSGAAAAQRKPLYAQLYVQVLVAITVGILLGHYYPSIGESMKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDLQ
KVGRVAGKAMLYFLTFSTLALIIGLVVANVVQPGAGFNIDPATLDASTVNTYAAKAHDQSVTGFLMNIIPGTIVGAFADG
DILQVLFFSVLFGIALALVGDKGAPVLNFLQALMAPMFKLVSVLMKAAPIGAFGAMAFTIGKYGIGSVINLAMLVGTFYA
TSLLFVFVVLGAVCRYNGFSILSLLRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNIHLSMGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNF
IGNAVATLVVARWEGELDEAKLARALAGTADDSLPADIVPAE

Sequences:

>Translated_442_residues
MHIADQSGAAAAQRKPLYAQLYVQVLVAITVGILLGHYYPSIGESMKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDLQ
KVGRVAGKAMLYFLTFSTLALIIGLVVANVVQPGAGFNIDPATLDASTVNTYAAKAHDQSVTGFLMNIIPGTIVGAFADG
DILQVLFFSVLFGIALALVGDKGAPVLNFLQALMAPMFKLVSVLMKAAPIGAFGAMAFTIGKYGIGSVINLAMLVGTFYA
TSLLFVFVVLGAVCRYNGFSILSLLRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNIHLSMGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNF
IGNAVATLVVARWEGELDEAKLARALAGTADDSLPADIVPAE
>Mature_442_residues
MHIADQSGAAAAQRKPLYAQLYVQVLVAITVGILLGHYYPSIGESMKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDLQ
KVGRVAGKAMLYFLTFSTLALIIGLVVANVVQPGAGFNIDPATLDASTVNTYAAKAHDQSVTGFLMNIIPGTIVGAFADG
DILQVLFFSVLFGIALALVGDKGAPVLNFLQALMAPMFKLVSVLMKAAPIGAFGAMAFTIGKYGIGSVINLAMLVGTFYA
TSLLFVFVVLGAVCRYNGFSILSLLRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNIHLSMGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNF
IGNAVATLVVARWEGELDEAKLARALAGTADDSLPADIVPAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane. This transport system plays an important role in the energy supply of rhizobium-legume symbionts

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI194239697, Length=462, Percent_Identity=25.974025974026, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI169790839, Length=444, Percent_Identity=26.5765765765766, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI40254478, Length=429, Percent_Identity=25.8741258741259, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4827012, Length=408, Percent_Identity=27.6960784313726, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI66773030, Length=411, Percent_Identity=25.5474452554745, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI21314632, Length=433, Percent_Identity=25.8660508083141, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI5032093, Length=344, Percent_Identity=29.0697674418605, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI223468566, Length=330, Percent_Identity=29.0909090909091, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI223468564, Length=300, Percent_Identity=30.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI262359914, Length=404, Percent_Identity=25, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=30.5389221556886, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789947, Length=419, Percent_Identity=61.3365155131265, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1790514, Length=434, Percent_Identity=40.0921658986175, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788024, Length=402, Percent_Identity=26.3681592039801, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537407, Length=405, Percent_Identity=27.1604938271605, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71996953, Length=452, Percent_Identity=27.4336283185841, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=451, Percent_Identity=25.0554323725055, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=423, Percent_Identity=25.531914893617, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983099, Length=423, Percent_Identity=25.531914893617, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=489, Percent_Identity=24.5398773006135, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=453, Percent_Identity=24.2825607064018, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583025, Length=402, Percent_Identity=25.1243781094527, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17137668, Length=402, Percent_Identity=25.1243781094527, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24583023, Length=402, Percent_Identity=25.1243781094527, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI281360483, Length=450, Percent_Identity=24, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137666, Length=450, Percent_Identity=24, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281360481, Length=450, Percent_Identity=24, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA2_RHILO (Q986R8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107599.1
- ProteinModelPortal:   Q986R8
- SMR:   Q986R8
- GeneID:   1230254
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll7237
- NMPDR:   fig|266835.1.peg.5700
- HOGENOM:   HBG739804
- OMA:   AGCKRSV
- ProtClustDB:   PRK01663
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46095; Mature: 46095

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15d212a4)-; HASH(0x1785313c)-; HASH(0x171de20c)-; HASH(0x177a29e4)-; HASH(0x16695fb4)-; HASH(0x17786e80)-; HASH(0x1744f78c)-; HASH(0x17854124)-; HASH(0x17786ad8)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968