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The map label for this gene is ykfB [H]

Identifier: 13475907

GI number: 13475907

Start: 5851819

End: 5852958

Strand: Reverse

Name: ykfB [H]

Synonym: mll7089

Alternate gene names: 13475907

Gene position: 5852958-5851819 (Counterclockwise)

Preceding gene: 13475916

Following gene: 13475906

Centisome position: 83.19

GC content: 59.91

Gene sequence:

>1140_bases
ATGAGCCTTTCGCATTTTCGTATAACCCGATTTCAGTTCGCCCGCGACCGGGTAATCGGGGACAGTCAGGTTCGGGCGGA
CGATGTCAACGTCGCTGCGCTGGAACTCGTTTCCGAAAGTGGCGAAGTGGGGCTGGGATTCATCCAGACGCTCTTCAATC
CACTGCCTGATCAACAGGAGATCGAGAGCGTTTTCGAACATGAGGTTTGGCCTAGCTTGAAGGGAAACAGAGCGATCGCC
CTGGTTCACCGCGTGAACCGCCCGCGTGGCGGCAATCAACGGGCTTACTCCCTGCCCTTTCACGAAGCGGTTCAAGTGGC
GCTTTGGGATCTTGCAGCGAAAGAAGCTGGCCTGCCCCTACATGTCTTGCTCGGCAGTCGCCGCAACCGGGTGAAAGCCT
ATGCCAGCGGCCTCGATTTTCACCTCGACGACGATGCCTTCGTCTCGTTGTTCTCCCACGCAGCGTCGATTGGCTACTCG
GCTTTCAAGATCAAGGTCGGCCACCGGGATTTCGACCGCGATCTGCGGCGCCTGGAATTGTTGAAGACCTGTGTCCCGGC
CGGGTCCAAAGTCATGATCGATCCGAACGAGGCCTGGACCTCGAAGGAGGCGCTGACGAAATTGGTGGCGATCCGCGAGG
CCGGCCACGATCTTCTGTGGGTCGAGGATCCGATCCTGCGTCACGACCATGACGGGCTGAGAACACTCAGGCATGCCGTC
ACATGGACCCAGATCAACAGCGGCGAGTATCTGGATCTCCAGGGCAAACGGCTGCTCCTGGAAGCCCATGCGGCCGATAT
CCTGAATGTCCACGGCCAGGTGACGGATGTCATGCGCATCGGCTGGCTGGCCGCCGAACTCGGGATACCAATCAGCATCG
GCAACACGTTCCTGGAGGCTGGCGTGCACATGGCGGTTGCGCTTCCCGAGGTCGAATGGCTGGAATATTCATTTCAGAAC
TTCGATCATCTGGTCGAACAGCCTATCGAAATCCGGGACGGCTACGCCTATGCGCCCGATCGGCCGGGCCATGGATTGGT
CCTGTCGGAAAAAGCGCGGGGCGAATGGAGCAGGCCAAGGCGCCTGGCACGGTCGGAGCTTGGCGCCGCGCCGGAAAATC
CACGCCTTCCCGCGAAGTAG

Upstream 100 bases:

>100_bases
TTTTGCTTTGCCCGCCACTTGCGGCCCCTCCATTGGTCCGGTAGTTAGATACCATCTGATAGATCAGTTGGATATCGTAA
CCGATCAGAAAGCCCCTGTG

Downstream 100 bases:

>100_bases
TTGAGAGGCTATAGCCGAAGACTACCGTCAGCAGCGCGAGGGATTGACCGCGACGCCGCTTCAAGCGATCGCAGCTCGGC
TTCCGGCCGGATCACGGCGA

Product: mandelate racemase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSLSHFRITRFQFARDRVIGDSQVRADDVNVAALELVSESGEVGLGFIQTLFNPLPDQQEIESVFEHEVWPSLKGNRAIA
LVHRVNRPRGGNQRAYSLPFHEAVQVALWDLAAKEAGLPLHVLLGSRRNRVKAYASGLDFHLDDDAFVSLFSHAASIGYS
AFKIKVGHRDFDRDLRRLELLKTCVPAGSKVMIDPNEAWTSKEALTKLVAIREAGHDLLWVEDPILRHDHDGLRTLRHAV
TWTQINSGEYLDLQGKRLLLEAHAADILNVHGQVTDVMRIGWLAAELGIPISIGNTFLEAGVHMAVALPEVEWLEYSFQN
FDHLVEQPIEIRDGYAYAPDRPGHGLVLSEKARGEWSRPRRLARSELGAAPENPRLPAK

Sequences:

>Translated_379_residues
MSLSHFRITRFQFARDRVIGDSQVRADDVNVAALELVSESGEVGLGFIQTLFNPLPDQQEIESVFEHEVWPSLKGNRAIA
LVHRVNRPRGGNQRAYSLPFHEAVQVALWDLAAKEAGLPLHVLLGSRRNRVKAYASGLDFHLDDDAFVSLFSHAASIGYS
AFKIKVGHRDFDRDLRRLELLKTCVPAGSKVMIDPNEAWTSKEALTKLVAIREAGHDLLWVEDPILRHDHDGLRTLRHAV
TWTQINSGEYLDLQGKRLLLEAHAADILNVHGQVTDVMRIGWLAAELGIPISIGNTFLEAGVHMAVALPEVEWLEYSFQN
FDHLVEQPIEIRDGYAYAPDRPGHGLVLSEKARGEWSRPRRLARSELGAAPENPRLPAK
>Mature_378_residues
SLSHFRITRFQFARDRVIGDSQVRADDVNVAALELVSESGEVGLGFIQTLFNPLPDQQEIESVFEHEVWPSLKGNRAIAL
VHRVNRPRGGNQRAYSLPFHEAVQVALWDLAAKEAGLPLHVLLGSRRNRVKAYASGLDFHLDDDAFVSLFSHAASIGYSA
FKIKVGHRDFDRDLRRLELLKTCVPAGSKVMIDPNEAWTSKEALTKLVAIREAGHDLLWVEDPILRHDHDGLRTLRHAVT
WTQINSGEYLDLQGKRLLLEAHAADILNVHGQVTDVMRIGWLAAELGIPISIGNTFLEAGVHMAVALPEVEWLEYSFQNF
DHLVEQPIEIRDGYAYAPDRPGHGLVLSEKARGEWSRPRRLARSELGAAPENPRLPAK

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 42457; Mature: 42326

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSHFRITRFQFARDRVIGDSQVRADDVNVAALELVSESGEVGLGFIQTLFNPLPDQQE
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
IESVFEHEVWPSLKGNRAIALVHRVNRPRGGNQRAYSLPFHEAVQVALWDLAAKEAGLPL
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEE
HVLLGSRRNRVKAYASGLDFHLDDDAFVSLFSHAASIGYSAFKIKVGHRDFDRDLRRLEL
EEEECCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
LKTCVPAGSKVMIDPNEAWTSKEALTKLVAIREAGHDLLWVEDPILRHDHDGLRTLRHAV
HHHHCCCCCEEEECCCHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHCCCHHHHHHHHHH
TWTQINSGEYLDLQGKRLLLEAHAADILNVHGQVTDVMRIGWLAAELGIPISIGNTFLEA
EEEEECCCCEEECCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECHHHHHC
GVHMAVALPEVEWLEYSFQNFDHLVEQPIEIRDGYAYAPDRPGHGLVLSEKARGEWSRPR
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCEEEECCCCCCCHHHH
RLARSELGAAPENPRLPAK
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SLSHFRITRFQFARDRVIGDSQVRADDVNVAALELVSESGEVGLGFIQTLFNPLPDQQE
CCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
IESVFEHEVWPSLKGNRAIALVHRVNRPRGGNQRAYSLPFHEAVQVALWDLAAKEAGLPL
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEE
HVLLGSRRNRVKAYASGLDFHLDDDAFVSLFSHAASIGYSAFKIKVGHRDFDRDLRRLEL
EEEECCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
LKTCVPAGSKVMIDPNEAWTSKEALTKLVAIREAGHDLLWVEDPILRHDHDGLRTLRHAV
HHHHCCCCCEEEECCCHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHCCCHHHHHHHHHH
TWTQINSGEYLDLQGKRLLLEAHAADILNVHGQVTDVMRIGWLAAELGIPISIGNTFLEA
EEEEECCCCEEECCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECHHHHHC
GVHMAVALPEVEWLEYSFQNFDHLVEQPIEIRDGYAYAPDRPGHGLVLSEKARGEWSRPR
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCEEEECCCCCCCHHHH
RLARSELGAAPENPRLPAK
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]